Apidologie

https://www.apidologie.org/

List of Papers (Total 1,007)

Molecular cloning, expression and oxidative stress response of the vitellogenin Gene (AccVg) from Apis cerana cerana

Vitellogenin (Vg) is a yolk precursor protein in most oviparous females. However, Vg has not been studied in the Apis cerana cerana. In this work, the Vg gene of the A. cerana cerana has been cloned and sequenced. The gene codes for a protein consisting of 1770 amino acids in seven exons with a predicted molecular mass and isoelectric point of 200 kDa and 6.46, respectively...

Leaf foraging sources of leafcutter bees in a tropical environment: implications for conservation

Leafcutter bees collect leaf discs to encase brood cells. However, our understanding of their use of plants as nesting resources, which is critical for their conservation, is poor. We followed plants and observed bees cutting leaves to understand the leaf and plant traits of the leaf forage plants of Megachile spp. We studied whether the leaf size explains the cut size and the...

In vitro study of the antimicrobial activity of European propolis against Paenibacillus larvae

Typical European propolis is an antibiotic substance derived from the resin of poplar, birch, and aspen buds. Here, we tested the ability of propolis originating from the resins of these plants to inhibit the in vitro growth of Paenibacillus larvae, the organism that causes American foulbrood, a fatal honeybee larval disease. The study involved GC-MS analysis of extracts from...

Influence of different pollen diets on the development of hypopharyngeal glands and size of acid gland sacs in caged honey bees (Apis mellifera)

We examined the consumption rate of protein diets in caged and free-flying honey bees, amino acid composition of diets, and diet effects on gland development. The effect of seven different diets (sugar solution only, Feedbee®, Helianthus pollen, Sinapis pollen, Asparagus pollen, Castanea pollen, and mixed pollen diet) on the development of the hypopharyngeal (HPG) and acid glands...

Honey bee gut microbial communities are robust to the fungicide Pristine® consumed in pollen

Honey bees that consume pollen with sublethal levels of the fungicide Pristine® can have reduced pollen digestion, lower ATP synthesis, and in many ways resemble malnourished bees. Reduced nutrient acquisition in bees exposed to Pristine® might be due to the effects of this fungicide on the composition of gut microbial communities. Two experiments were conducted in two separate...

Pathogens, parasites, and parasitoids associated with bumble bees (Bombus spp.) from Uruguay

As elsewhere in the world, bumble bees play a vital role as pollinators in Uruguay, but knowledge on their health status is still limited. Between September 2012 and May 2013, 403 adult individuals of the two species of Bombus known for the country (Bombus atratus, Bombus bellicosus) were collected in six localities. We found that 177 (119 B. atratus, 58 B. bellicosus) were...

Distributional analysis of Melipona stingless bees (Apidae: Meliponini) in Central America and Mexico: setting baseline information for their conservation

Melipona stingless bee species of Central America and Mexico are important ecologically, culturally, and economically as pollinators and as a source of food and medicine. Despite their importance, however, information on their distribution patterns is limited. We propose potential distribution models for 11 Melipona species in the region. Our main findings support previous data...

Sperm parameters of honeybee drones exposed to imidacloprid

The objective of this study was to evaluate the effects of chronical exposure of honeybee drones to environmental (5 ppb) and non-environmental concentration (200 ppb) of imidacloprid (IMD) on sperm concentration, motility, viability, and mitochondrial membrane potential measured in semen obtained from 180 drones originating from 18 colonies. The results demonstrate that IMD...

Bumblebee sonication behavior changes with plant species and environmental conditions

Bumblebees collect pollen from some plants by grasping flowers and vibrating their flight muscles—a behavior termed buzz pollination, or sonication. The extent to which bees can and do alter their sonication has been scarcely documented. We show that bumblebees are capable of changing their vibration frequency. In two field studies, we found that relative humidity, date, and bee...

Worker size in honeybees and its relationship with season and foraging distance

Workers in many eusocial insect species show considerable size variation within a colony. Honeybees (Apis mellifera) vary little in size compared to other eusocial bee species, but there is evidence for a link between worker size and behaviour. In this study, we investigated how size variation and the average size of honeybee foragers change during a foraging season. We also...

Temporal niche overlap and distinct bee ability to collect floral resources on three species of Brazilian Malpighiaceae

Spatial and temporal limited resource and niche overlap studies have been widely used to explain resource-sharing in community ecology. Furthermore, morphological and behavioral differences among species are relevant to predict how they may share niche availabilities. We evaluated temporal niche overlap and the ability of visitors to collect floral resources on three...

Newly emerged workers of the stingless bee Scaptotrigona aff. depilis prefer stored pollen to fresh pollen

Like in honey bees, the pollen collected and stored by stingless bees usually undergoes a fermentation process characterized by increase in moisture, lactic acid, and proliferation of microorganisms. We tested whether 2-day-old Scaptotrigona aff. depilis workers were more attracted to and consumed fermented pollen from old pollen pots (>7 days) or fresh pollen collected from the...

Introduction of Aethina tumida (Coleoptera: Nitidulidae) in the regions of Calabria and Sicily (southern Italy)

Aethina tumida (small hive beetle, SHB) was first detected in September 2014 in Calabria region, southern Italy, and in a single apiary in Sicily in November 2014. In September 2015, SHB was again recorded in Calabria, and in 2016, only sentinel honey bee nucleus colonies were found to be infested. Its phylogenetic relationship and possible origin were investigated comparing the...

Honeybee worker larvae perceive queen pheromones in their food

Honeybee workers develop from fertilised eggs, but those reared in a queenless colony develop into ‘rebel’ workers, which are more queen-like than typical workers. Rebels develop after an old queen leaves with a swarm and before a new queen hatches. We hypothesised that larval food lacking queen mandibular pheromones trigger the rebel phenotype. Larvae reared under queenright or...

Bigger and sweeter passion fruits: effect of pollinator enhancement on fruit production and quality

Brazil stands out as the world’s largest producer of yellow passion fruit, a species of economic interest which, due to its self-incompatibility, depends on pollinators for fruit formation. The main pollinators of passion fruit are large solitary bees of the genus Xylocopa, commonly known as carpenter bees, which are potential candidates for management. Passion fruit crops...

Observations on midgut of Apis mellifera workers (Hymenoptera: Apoidea) under controlled acute exposures to a Bacillus thuringiensis-based biopesticide

Morphostructural investigations have been carried out on Apis mellifera workers treated with single controlled acute exposures to a biopesticide containing Bacillus thuringiensis (Bt), to detect midgut changes until 96 h. Our findings show concentration-dependence of these changes, reflecting in different degrees on both mortality and behaviour. In particular, some midgut changes...

Timing and size of daily pollen meals eaten by adult females of a solitary bee (Nomia melanderi) (Apiformes: Halictidae)

Pollen feeding by solitary bees has been studied mainly with their larvae, overlooking pollen feeding by adults. To address this knowledge gap, we determined the amounts and temporal (daily and lifetime) schedules of pollen consumption by freely nesting, adult females of the alkali bee (Nomia melanderi, Halictidae). Nesting females of known ages were taken at different hours of...

Distance between honey bee Apis mellifera colonies regulates populations of Varroa destructor at a landscape scale

Inter-colony distance of Apis mellifera significantly affects colony numbers of the parasitic mite Varroa destructor. We set up 15 apiaries, each consisting of two colonies. Each apiary pair was assigned an inter-colony distance of 0, 10, or 100 m. Colonies were rendered nearly mite-free, then one colony in each pair was seeded with 300 female mites (mite-donor colony), while the...

Ecology and nesting biology of the wood-boring bee Trichothurgus laticeps (Hymenoptera: Megachilidae) in a Monte desert reserve in mid-western Argentina

Studying bee nests can enlighten our understanding of feeding specialization and phylogenetic relationships of bees. We studied the nesting and feeding habits of Trichothurgus laticeps in the Monte desert ecosystem. Our results show that T. laticeps is attracted to pre-existing cavities in wood (trap nests), which were further excavated for nest construction; nest cells are...

Multiyear survey targeting disease incidence in US honey bees

The US National Honey Bee Disease Survey sampled colony pests and diseases from 2009 to 2014. We verified the absence of Tropilaelaps spp., the Asian honey bee (Apis cerana), and slow bee paralysis virus. Endemic health threats were quantified, including Varroa destructor, Nosema spp., and eight honey bee viruses. Varroa loads varied across years, with annual fall peaks; Nosema...

High prevalence of Miconia chamissois (Melastomataceae) pollen in brood cell provisions of the orchid bee Euglossa townsendi in São Paulo State, Brazil

Females of bee species can present a temporal specialized behavior in the food collection for their offspring. We studied the food of Euglossa townsendi immatures from nests established in trap-nests disposed in a hygrophilous forest in Brazil, between March 2010 and February 2012. The pollinic material remaining in the brood cells were acetolysed and identified by comparison...

Combined effect of pollen quality and thiamethoxam on hypopharyngeal gland development and protein content in Apis mellifera

Pollen diet is essential to nurse bees to develop hypopharyngeal glands (HPGs) and produce royal jelly to feed larvae. The effect of different diet regimes combined with pesticide exposure on the development of HPGs in honeybees was studied. In particular, a high-quality pollen mixture (high pollen diversity and protein content) and a low-quality diet (low pollen diversity and...