Apidologie

https://www.apidologie.org/

List of Papers (Total 1,007)

Pathogen prevalence and abundance in honey bee colonies involved in almond pollination

Honey bees are important pollinators of agricultural crops. Since 2006, US beekeepers have experienced high annual honey bee colony losses, which may be attributed to multiple abiotic and biotic factors, including pathogens. However, the relative importance of these factors has not been fully elucidated. To identify the most prevalent pathogens and investigate the relationship...

Pharmacophagy and pharmacophory: mechanisms of self-medication and disease prevention in the honeybee colony (Apis mellifera)

Apitherapy promises cures for diseases in human folk medicine, but the effects of honeybee produced and foraged compounds on bee health are less known. Yet, hive products should chiefly facilitate medication and sanitation of the honeybees themselves rather than other organisms. We here review the impact of both self-produced gland secretions and foraged hive products...

Impact of sugar syrup and pollen diet on the bacterial diversity in the gut of indoor-reared bumblebees (Bombus terrestris)

In this project, we showed the influence of diet on the microbial composition in the gut of indoor-reared bumblebees (Bombus terrestris), using Illumina MiSeq 16S ribosomal RNA (rRNA) sequencing. Three sugar syrups and sterilized pollen from three suppliers were tested. Different effects were observed depending on the developmental stage of the gut microbiota at the start of the...

In vitro rearing of stingless bee queens and their acceptance rate into colonies

The in vitro rearing of queen bees can improve the management and conservation of pollinator insects. Here, we have developed an in vitro queen-rearing protocol for Plebeia droryana (Apidae, Meliponini). Firstly, we evaluated the amount of food offered naturally to P. droryana queen larvae. After, we grafted P. droryana larvae onto acrylic plates which were kept in constant...

Testing dose-dependent effects of stacked Bt maize pollen on in vitro-reared honey bee larvae

In agricultural landscapes all over the world, honey bees are exposed to genetically modified (GM) pollen. Stacked Bt-maize varieties combine multiple Bt proteins against different insect taxa with herbicide resistance. To test for potential effects on non-target organisms, we conducted an in vitro larvae-rearing experiment where we fed increasing amounts of stacked Bt pollen to...

Total antioxidant capacity of honeybee haemolymph in relation to age and exposure to pesticide, and comparison to antioxidant capacity of seminal plasma

Oxidative stress is defined as a disturbance in the balance between the production of reactive oxygen species and antioxidant defences. We measured total antioxidant capacity (TAC) in honeybee haemolymph and seminal plasma and analysed TAC of haemolymph in relation to age and exposure to pesticide. TAC of haemolymph increased with age of bees (1.18 vs 1.97 mM of (±)-6-hydroxy-2,5...

Innate expression of antimicrobial peptides does not explain genotypic diversity in resistance to fungal brood parasites in the honey bee

The genetic basis of host resistance to parasites is a fundamental aspect of host-parasite co-evolution, yet the precise mechanisms often remain unclear. Here, we follow on from a previous study on the genetically mediated variation in resistance to two common fungal brood parasites that cause chalkbrood and stonebrood in the honey bee. We assessed whether genetically mediated...

An integrative approach identifies a new species of bumblebee (Hymenoptera: Apidae: Bombini) from northeastern Brazil

Here, we describe a new species of genus Bombus Latreille, 1802 from northeastern Brazil, Bombus (Thoracobombus) applanatus Oliveira, Françoso & Arias, sp. nov. Molecular analysis was initially performed to confirm the new species placement within the genus Bombus. Afterward, we performed an integrative approach combining molecular data (DNA barcoding and two nuclear regions) and...

Honey bee colonies provided with natural forage have lower pathogen loads and higher overwinter survival than those fed protein supplements

Malnutrition is a major cause of colony losses. In managed hives, bees are fed protein supplements (PS) during pollen shortages. If bees were provided with natural forage instead of PS, would they have lower pathogen levels and higher queen and colony survival? We addressed this question by either providing colonies with forage (Brassica rapa—rapini) or feeding them PS from...

Mitochondrial DNA variations in Korean Apis cerana (Hymenoptera: Apidae) and development of another potential marker

The geographic relationships and biogeography of Apis cerana have been studied extensively, but Korean populations have not been investigated thoroughly. We sequenced the non-coding region between the tRNALeu and COII mitochondrial (mt) genes (herein named NC2) of Korean samples, along with the samples from seven Asian localities (China, Vietnam, and Thailand). Four undiscovered...

Per-visit pollinator performance and regional importance of wild Bombus and Andrena (Melandrena) compared to the managed honey bee in New York apple orchards

Declines in honey bee health and increasing demand for pollination services highlight a need to optimize crop pollination by wild bees. Apple is an economically important crop in eastern North America, requires insect pollination, and is visited by a diverse bee fauna, but a direct assessment of wild bee pollination in apple orchards is lacking. We combined measurements of two...

Variation in maternal solitary bee nest defence related to nest state

Parental protection of offspring is found in numerous animal species. Protection provides offspring with a greater chance of surviving to be able to reproduce, while at the same time, often posing a cost to the parent. Therefore, the net value of defence for the parent can vary depending on the developmental stage of the offspring and their ability to defend themselves. For...

Delimiting the species within the genus Austroplebeia, an Australian stingless bee, using multiple methodologies

Austroplebeia Moure is an Australian stingless bee genus. The current descriptions for the species within this genus are inadequate for the identification of specimens in either the field or the laboratory. Here, using multiple diagnostic methodologies, we attempted to better delimit morphologically identified groups within Austroplebeia. First, morphological data, based on...

Assessing the role of β-ocimene in regulating foraging behavior of the honey bee, Apis mellifera

The larval pheromone (E)-β-ocimene (EBO) inhibits worker ovary development and accelerates the behavioral transition from nursing to foraging in the honey bee, Apis mellifera. Because EBO diffuses throughout the hive, it has been hypothesized that honey bee workers can use it to indirectly assess brood abundance. Here, we evaluate this hypothesis by testing whether EBO plays a...

Establishment risk of the commercially imported bumblebee Bombus terrestris dalmatinus—can they survive UK winters?

Bumblebees are regularly exported to countries outside their native range for the purposes of commercial pollination. In contrast to the tight regulations imposed on biological control introductions, the movement of bumblebees has largely been without risk assessment. This study represents the first formal assessment of establishment risk for Bombus terrestris dalmatinus in the...

Discovery of Lake Sinai virus and an unusual strain of acute bee paralysis virus in West African apiaries

Samples of bees and honey from 101 apiaries were collected in the West African Republic of Benin. Visual inspection revealed high Varroa destructor and Aethina tumida infestations and low Oplostomus fuligineus infestations. A pathogen screen based on bacterial/fungal cultures or microscopic examinations revealed the absence of American and European foulbrood, chalkbrood disease...

Comparison of the nutrient composition of royal jelly and worker jelly of honey bees (Apis mellifera)

In this study, the chemical and mineral composition and trace elements in royal jelly (RJ) and worker jelly (WJ) and in royal jelly on particular days (only-2-day RJ [O2d], only-3-day RJ [O3d] and only-4-day RJ [O4d]) were determined. Significant differences in levels of moisture, protein, 10-hydroxy-2-decenoic acid (10-HDA), fructose (F) and glucose (G) were found between the RJ...

Pollen nutrition in honey bees (Apis mellifera): impact on adult health

Pollen is the main protein source for honey bees, and its quality and digestibility are important factors for bee health. We compared the effects of eight different mixed-pollen diets on queenless adult survival and physiology. The mixtures represented the pollen source for bees in a natural environment. They consisted of three to five familial pollen types, and the protein...

Hybrid origins of Australian honeybees (Apis mellifera)

With increased globalisation and homogenisation, the maintenance of genetic integrity in local populations of agriculturally important species is of increasing concern. The western honeybee (Apis mellifera) provides an interesting perspective as it is both managed and wild, with a large native range and much larger introduced range. We employed a newly created 95 single...

The development of HopGuard® as a winter treatment against Varroa destructor in colonies of Apis mellifera

The development of new veterinary medicinal products to control Varroa destructor is very important for the successful management of honey bee colonies. We tested natural plant compounds (hop acids) as active ingredients for a new veterinary drug for Varroa control. Tolerability of bees and efficacy against mites was tested in the laboratory and in the field. In laboratory tests...

Identification of cryptic species and morphotypes in male Euglossa: morphometric analysis of forewings (Hymenoptera: Euglossini)

Males of sibling orchid bees Euglossa viridissima and Euglossa dilemma are morphologically cryptic, except for the number and shape of mandibular teeth. An alternative morph of E. viridissima has a third tooth similar to males of E. dilemma. We used this model system to evaluate the potential of wing morphometrics for the resolution of these groups. We found differences in the...

Monitoring colony phenology using within-day variability in continuous weight and temperature of honey bee hives

Continuous weight and temperature data were collected for honey bee hives in two locations in Arizona, and those data were evaluated with respect to separate measurements of hive phenology to develop methods for noninvasive hive monitoring. Weight and temperature data were divided into the 25-h running average and the daily within-day changes, or “detrended” data. Data on adult...

Prevalence and reproduction of Tropilaelaps mercedesae and Varroa destructor in concurrently infested Apis mellifera colonies

The prevalence of Tropilaelaps mercedesae and Varroa destructor in concurrently infested A. mellifera colonies in Thailand was monitored. We also assessed the fecundity of T. mercedesae and V. destructor in naturally infested brood and in brood cells deliberately infested with both mite genera. Results showed that the natural co-infestation of an individual brood cell by both...

Male mites (Varroa destructor) perceive the female sex pheromone with the sensory pit organ on the front leg tarsi

Varroa destructor males are attracted by a volatile sex pheromone of female mites. We assume that this pheromone is perceived by the sensory pit organ on the front leg tarsi. To test this hypothesis, the front legs of the males were varnished with nail polish. The behavior of the thus treated males toward attractive female mites was analyzed in our mating bioassay and compared to...

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