Molecular Imaging and Biology

http://link.springer.com/journal/11307

List of Papers (Total 192)

The Value of Radiologic Interventions and 18F-DOPA PET in Diagnosing and Localizing Focal Congenital Hyperinsulinism: Systematic Review and Meta-Analysis

Purpose This systematic review and meta-analysis aimed to quantify the diagnostic performance of pancreatic venous sampling (PVS), selective pancreatic arterial calcium stimulation with hepatic venous sampling (ASVS), and 18F-DOPA positron emission tomography (PET) in diagnosing and localizing focal congenital hyperinsulinism (CHI). Procedures This systematic review and meta...

Intraoperative Fluorescence Imaging of Peripheral and Central Nerves Through a Myelin-Selective Contrast Agent

Purpose Patients suffer from complications as a result of unintentional nerve damage during surgery. We focus on improving intraoperative visualization of nerves through the use of a targeted fluorophore and optical imaging instrumentation. Procedure A myelin-targeting fluorophore, GE3111, was synthesized, characterized for its optical and myelin-binding properties using purified...

P-Glycoprotein Function at the Blood–Brain Barrier: Effects of Age and Gender

Purpose P-glycoprotein (Pgp) is an efflux transporter involved in transport of several compounds across the blood–brain barrier (BBB). Loss of Pgp function with increasing age may be involved in the development of age-related disorders, but this may differ between males and females. Pgp function can be quantified in vivo using (R)-[11C]verapamil and positron emission tomography...

Validity of Simplified 3′-Deoxy-3′-[18F]Fluorothymidine Uptake Measures for Monitoring Response to Chemotherapy in Locally Advanced Breast Cancer

Purpose Positron emission tomography using 3′-deoxy-3′-[18F]fluorothymidine ([18F]FLT) has been suggested as a means for monitoring response to chemotherapy. The aim of this study was to evaluate the validity of simplified uptake measures for assessing response to chemotherapy using [18F]FLT in locally advanced breast cancer (LABC). Procedures Fifteen LABC patients underwent...

Near-Infrared Fluorescence Molecular Imaging of Ductal Carcinoma In Situ with CD44v6-Specific Antibodies in Mice: A Preclinical Study

Purpose The purpose of this study was to develop a molecular imaging technique using tracers specific for ductal carcinoma in situ (DCIS) to improve visualization and localization of DCIS during surgery. As CD44v6 is frequently expressed in DCIS, we used near-infrared fluorescently labeled CD44v6-targeting antibodies for detection of DCIS. Procedure Mice bearing orthotopically...

99mTc-labeled Rituximab for Imaging B Lymphocyte Infiltration in Inflammatory Autoimmune Disease Patients

Purpose The rationale of the present study was to radiolabel rituximab with 99m-technetium and to image B lymphocytes infiltration in the affected tissues of patients with chronic inflammatory autoimmune diseases, in particular, the candidates to be treated with unlabelled rituximab, in order to provide a rationale for ‘evidence-based’ therapy. Procedures Rituximab was labelled...

Automated Bone Volume and Thickness Measurements in Small Animal Whole-Body MicroCT Data

Purpose Quantification of osteolysis is crucial for monitoring treatment effects in preclinical research and should be based on MicroCT data rather than conventional 2D radiographs to obtain optimal accuracy. However, data assessment is greatly complicated in the case of 3D data. This paper presents an automated method to follow osteolytic lesions quantitatively and visually over...

Albumin-Binding Domain Conjugate for Near-Infrared Fluorescence Lymphatic Imaging

Purpose The aim of this study was to develop and characterize a novel peptide imaging agent for noninvasive near-infrared fluorescence imaging of protein transport by the lymphatics. An imaging agent consisting of a cyclic albumin-binding domain (cABD) peptide, with sequence, Arg-Leu-Ile-Glu-Asp-Ile-Cys-Leu-Pro-Arg-Trp-Gly-Cys-Leu-Trp-Glu-Asp-Asp-Lys, was conjugated to a near...

Dual-Labeling Strategies for Nuclear and Fluorescence Molecular Imaging: A Review and Analysis

Molecular imaging is used for the detection of biochemical processes through the development of target-specific contrast agents. Separately, modalities such as nuclear and near-infrared fluorescence (NIRF) imaging have been shown to non-invasively monitor disease. More recently, merging of these modalities has shown promise owing to their comparable detection sensitivity and...

A Cone-Shaped Phantom for Assessment of Small Animal PET Scatter Fraction and Count Rate Performance

Purpose Positron emission tomography (PET) image quality deteriorates as the object size increases owing to increased detection of scattered and random events. The characterization of the scatter component in small animal PET imaging has received little attention owing to the small scatter fraction (SF) when imaging rodents. The purpose of this study is first to design and...

The Effect of Inhaled Gases on Ultrasound Contrast Agent Longevity In Vivo

Purpose The purpose of this study is to investigate the effect of the inhaled gas used alongside isoflurane in the anesthetization of small animals on the time-intensity curves (TICs) acquired from ultrasound contrast agents—microbubbles. Procedures TICs were recorded over the common iliac vein of 12 mice receiving Definity®. Animals were anesthetized with isoflurane, the...

Characterization of Human Osteoarthritic Cartilage Using Optical and Magnetic Resonance Imaging

Purpose Osteoarthritis (OA) is a degenerative disease starting with key molecular events that ultimately lead to the breakdown of the cartilage. The purpose of this study is to use two imaging methods that are sensitive to molecular and macromolecular changes in OA to better characterize the disease process in human osteoarthritic cartilage. Procedures Human femoral condyles were...

The World Molecular Imaging Society (WMIS)

At this years’ World Molecular Imaging Congress (WMIC), we announced the merger of the Academy of Molecular Imaging (AMI) and the Society for Molecular Imaging (SMI) into the WMIS. This merger combines two complimentary societies dedicated to the development, advancement, and clinical translation of the nascent field of Molecular Imaging.Both the AMI and SMI were established a...

Temporal Variations of Skin Pigmentation in C57Bl/6 Mice Affect Optical Bioluminescence Quantitation

Purpose Depilation-induced skin pigmentation in C57Bl/6 mice is a known occurrence, and presents a unique problem for quantitative optical imaging of small animals, especially for bioluminescence. The work reported here quantitatively investigated the optical attenuation of bioluminescent light due to melanin pigmentation in the skin of transgenic C57Bl/6 mice, modified such that...

Where It’s at Really Matters: In Situ In Vivo Vascular Endothelial Growth Factor Spatially Correlates with Electron Paramagnetic Resonance pO2 Images in Tumors of Living Mice

Purpose Tumor microenvironments show remarkable tumor pO2 heterogeneity, as seen in prior EPR pO2 images (EPROI). pO2 correlation with hypoxia response proteins is frustrated by large rapid pO2 changes with position. Procedures To overcome this limitation, biopsies stereotactically located in the EPROI were used to explore the relationship between vascular endothelial growth...

Monitoring the Effect of Targeted Therapies in a Gastrointestinal Stromal Tumor Xenograft Using a Clinical PET/CT

Purpose The purpose of this study is to assess treatment responses induced by the two tyrosine kinase inhibitors, Imatinib and Sunitinib, in a gastrointestinal stromal tumor (GIST) xenograft using a clinical positron emission tomography/computed tomography (PET/CT) scanner. Methods Nude mice bearing human GIST xenografts with mutations in exons 11 and 17 were randomly allocated...

Performance Evaluation of PETbox: A Low Cost Bench Top Preclinical PET Scanner

Purpose PETbox is a low cost bench top preclinical PET scanner dedicated to pharmacokinetic and pharmacodynamic mouse studies. A prototype system was developed at our institute, and this manuscript characterizes the performance of the prototype system. Procedures The PETbox detector consists of a 20 × 44 bismuth germanate crystal array with a thickness of 5 mm and cross-section...

Synthesis and Evaluation of 99mTc-Labelled Monoclonal Antibody 1D09C3 for Molecular Imaging of Major Histocompatibility Complex Class II Protein Expression

Purpose It is known that major histocompatibility complex class II protein HLA-DR is highly expressed in B-cell lymphomas and in a variety of autoimmune and inflammatory diseases. Therefore, a radiolabelled fully humanized IgG4 monoclonal antibody (mAb) can provide useful prognostic and diagnostic information. Aims of the present study were to radiolabel an anti-HLA-DR mAb with...