Molecular Imaging and Biology

http://link.springer.com/journal/11307

List of Papers (Total 192)

Intraoperative Multispectral Fluorescence Imaging for the Detection of the Sentinel Lymph Node in Cervical Cancer: A Novel Concept

Purpose Real-time intraoperative near-infrared fluorescence (NIRF) imaging is a promising technique for lymphatic mapping and sentinel lymph node (SLN) detection. The purpose of this technical feasibility pilot study was to evaluate the applicability of NIRF imaging with indocyanin green (ICG) for the detection of the SLN in cervical cancer. Procedures In ten patients with early...

Day-to-Day Test–Retest Variability of CBF, CMRO2, and OEF Measurements Using Dynamic 15O PET Studies

Purpose We assessed test–retest variability of cerebral blood flow (CBF), cerebral blood volume (CBV), cerebral metabolic rate of oxygen (CMRO2), and oxygen extraction fraction (OEF) measurements derived from dynamic 15O positron emission tomography (PET) scans. Procedures In seven healthy volunteers, complete test–retest 15O PET studies were obtained; test–retest variability and...

Multispectral Optoacoustic Tomography of Matrix Metalloproteinase Activity in Vulnerable Human Carotid Plaques

AimsElevated expression of cathepsins, integrins and matrix metalloproteinases (MMPs) is typically associated with atherosclerotic plaque instability. While fluorescent tagging of such molecules has been amply demonstrated, no imaging method was so far shown capable of resolving these inflammation-associated tags with high fidelity and resolution beyond microscopic depths. This...

Functional Phenotyping of the Maternal Albumin Turnover in the Mouse Placenta by Dynamic Contrast-Enhanced MRI

Purpose The purpose of this study was to develop a tool for functional phenotyping of the maternal circulation in the mouse placenta. Procedures In utero macromolecular dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) was performed on embryonic day 10.5 (E10.5), E13.5, and E18.5. Fluorescence analysis was also used for validation of the results. Results The initial...

Evaluation of Two Internalizing Carcinoembryonic Antigen Reporter Genes for Molecular Imaging

Purpose The objective of this article is to develop internalizing positron emission tomography (PET) reporter genes for tracking genetically modified T cells in vivo. Procedures The transmembrane and cytoplasmic domains of the human transferrin receptor (TfR) and CD5 were each fused to the carcinoembryonic (CEA) minigene N-A3 and expressed in Jurkat T cells. Internalization was...

Monitoring Proteins Using In Vivo Near-Infrared Time-Domain Optical Imaging after 2-O-Hexyldiglycerol-Mediated Transfer to the Brain

Purpose The aim of the present study was to gain insight into the penetration, biodistribution, and fate of globulins in the brain after 2-O-hexyldiglycerol-induced blood–brain barrier opening. Procedures The spatial distribution of fluorescence probes was investigated after blood–brain barrier opening with intracarotid 2-O-hexyldiglycerol injection. Fluorescence intensity was...

Conditional Bicistronic Cre Reporter Line Expressing Both Firefly Luciferase and β-galactosidase

Purpose The Cre-loxP system has become an important strategy for conditional gene deletion and conditional gene expression in genetically engineered mice. To evaluate Cre recombinase expression, we generated reporter mice that permit both noninvasive imaging in living animals and either ex vivo histochemical/immunohistochemical tissue transgene expression analysis or quantitative...

Interobserver Variability in Chest CT and Whole Body FDG-PET Screening for Distant Metastases in Head and Neck Cancer Patients

Purpose The aim of the study was to assess the interobserver variability in chest computed tomography (CT) and whole body 2-deoxy-2-[18F]fluoro-d-glucose positron emission tomography (FDG-PET) screening for distant metastases in head and neck squamous cell carcinoma (HNSCC) patients. Procedure Chest CT and whole body FDG-PET of 69 HNSCC patients with high-risk factors who...

Irradiation, Cisplatin, and 5-Azacytidine Upregulate Cytomegalovirus Promoter in Tumors and Muscles: Implementation of Non-invasive Fluorescence Imaging

Purpose The cytomegalovirus (CMV) promoter is one of the most commonly used promoters for expression of transgenes in mammalian cells. The aim of our study was to evaluate the role of methylation and upregulation of the CMV promoter by irradiation and the chemotherapeutic agent cisplatin in vivo using non-invasive fluorescence in vivo imaging. Procedures Murine fibrosarcoma LPB...

Two-Photon Intravital Multicolor Imaging Combined with Inducible Gene Expression to Distinguish Metastatic Behavior of Breast Cancer Cells In Vivo

Purpose The aim of this study is to use multicolor intravital imaging together with an inducible cell model to compare metastatic behavior of control and genetically modified breast cancer cell populations within the intact primary tumor of a mouse. Procedure GFP-MTLn3-ErbB1 cells were generated with doxycycline-regulated conditional transgene expression using lentiviral...

Single-Dose Intravenous Toxicity Study of IRDye 800CW in Sprague-Dawley Rats

Objective Fluorophore-labeled contrast imaging agents are moving toward clinical use for a number of applications. The near-infrared dye IRDye 800CW is frequently used in its N-hydroxysuccinamide (NHS) ester form for labeling these agents. Following conjugation or breakdown of a labeled ligand, excess NHS ester is converted to the carboxylate form. To prepare for clinical use as...

Impact of Dedicated Brain PET on Intended Patient Management in Participants of the National Oncologic PET Registry

PurposeThis study seeks to assess the impact of dedicated brain positron emission tomography (PET) with 2-deoxy-2-[18F]fluoro-d-glucose on intended management of patients with primary and metastatic brain tumors.ProceduresWe analyzed demographic characteristics and evaluated change in intended management after PET, using previously described metrics, for patients in the National...

Synthesis and Optimization of the Labeling Procedure of 99mTc-Hynic-Interleukin-2 for In vivo Imaging of Activated T lymphocytes

Introduction We have previously described the labeling of interleukin-2 (IL2) with 123I and 99mTc-N3S. Both radiopharmaceuticals were successfully applied in humans to image several inflammatory lesions and autoimmune diseases characterized by tissue infiltrating lymphocytes expressing the IL2 receptor (CD25). However, both radiopharmaceuticals had some specific disadvantages...

Quantitative Assessment of Diffusion-Weighted MR Imaging in Patients with Primary Rectal Cancer: Correlation with FDG-PET/CT

Purpose The aim of the study was to assess correlations between parameters on diffusion-weighted imaging and 2-deoxy-2-[18F]fluoro-d-glucose–positron emission tomography/computed tomography (FDG-PET/CT) in rectal cancer. Procedures Thirty-three consecutive patients with pathologically confirmed rectal adenocarcinoma were included in this study. Apparent diffusion coefficient (ADC...

Articulated Whole-Body Atlases for Small Animal Image Analysis: Construction and Applications

PurposeUsing three publicly available small-animal atlases (Sprague–Dawley rat, MOBY, and Digimouse), we built three articulated atlases and present several applications in the scope of molecular imaging.ProceduresMajor bones/bone groups were manually segmented for each atlas skeleton. Then, a kinematic model for each atlas was built: each joint position was identified and the...

[11C]acetate PET/CT Visualizes Skeletal Muscle Exercise Participation, Impaired Function, and Recovery after Hip Arthroplasty; First Results

Purpose Based on skeletal muscle acetate physiology we aimed studying muscle function after hip arthroplasty with [11C]acetate PET. Procedures Two male patients were investigated 3 and 12 weeks after hip arthroplasty with muscle [11C]acetate PET/CT performed at rest and exercise. Median muscle SUVmean were calculated on three non-consecutive transverse PET slices. Results The...

Non-invasive Optical Imaging of Muscle Pathology in mdx Mice Using Cathepsin Caged Near-Infrared Imaging

PurposeTo develop a reliable live-animal imaging method for monitoring muscle pathology in mouse models of myopathy.ProceduresA caged near-infrared Cathepsin B (CTSB) substrate, ProSense 680, is evaluated in the dystrophin deficient mdx mice, a genetic homologue of Duchenne muscular dystrophy via optical imaging.ResultsWe show high levels of infrared signal in dystrophic muscle...

Optical Image-guided Surgery—Where Do We Stand?

In cancer surgery, intra-operative assessment of the tumor-free margin, which is critical for the prognosis of the patient, relies on the visual appearance and palpation of the tumor. Optical imaging techniques provide real-time visualization of the tumor, warranting intra-operative image-guided surgery. Within this field, imaging in the near-infrared light spectrum offers two...