Molecular Imaging and Biology

http://link.springer.com/journal/11307

List of Papers (Total 189)

In Vivo Mouse Bioluminescence Tomography with Radionuclide-Based Imaging Validation

IntroductionBioluminescence imaging, especially planar bioluminescence imaging, has been extensively applied in in vivo preclinical biological research. Bioluminescence tomography (BLT) has the potential to provide more accurate imaging information due to its 3D reconstruction compared with its planar counterpart.MethodsIn this work, we introduce a positron emission tomography...

Image Derived Input Functions: Effects of Motion on Tracer Kinetic Analyses

Purpose To quantify the effects of motion affected image-derived input functions (IDIF) on the outcome of tracer kinetic analyses. Procedures Two simulation studies, one based on high and the other on low cortical uptake, were performed. Different degrees of rotational and axial translational motion were added to the final frames of simulated dynamic positron emission tomography...

Labeling Human Mesenchymal Stem Cells with Fluorescent Contrast Agents: the Biological Impact

Purpose This study aims to determine the effect of human mesenchymal stem cell (hMSC) labeling with the fluorescent dye DiD and the iron oxide nanoparticle ferucarbotran on chondrogenesis. Procedures hMSCs were labeled with DiD alone or with DiD and ferucarbotran (DiD/ferucarbotran). hMSCs underwent confocal microscopy, optical imaging (OI), and magnetic resonance (MR) imaging...

Evaluation of Modified PEG-Anilinoquinazoline Derivatives as Potential Agents for EGFR Imaging in Cancer by Small Animal PET

Purpose The in vivo evaluation of three modified polyethylene glycol (PEG)-anilinoquinazoline derivatives labeled with 124I, 18F, and 11C as potential positron emission tomography (PET) bioprobes for visualizing epidermal growth factor receptor (EGFR) in cancer using small animal PET. Procedures Xenograft mice with the human glioblastoma cell lines U138MG (lacking EGFR expression...

Bioluminescence Imaging of Angiogenesis in a Murine Orthotopic Pancreatic Cancer Model

PurposeAngiogenesis is essential for physiological processes as well as for carcinogenesis. New approaches to cancer therapy include targeting angiogenesis. One target is VEGF-A and its receptor VEGFR2. In this study, we sought to investigate pancreatic cancer angiogenesis in a genetically modified VEGFR2-luc-KI mouse.ProceduresLive in vivo bioluminescence imaging of angiogenesis...

Surface Feature-Guided Mapping of Cerebral Metabolic Changes in Cognitively Normal and Mildly Impaired Elderly

Purpose The aim of this study was to investigate the longitudinal positron emission tomography (PET) metabolic changes in the elderly. Procedures Nineteen nondemented subjects (mean Mini-Mental Status Examination 29.4 ± 0.7 SD) underwent two detailed neuropsychological evaluations and resting 2-deoxy-2-[F-18]fluoro-d-glucose (FDG)-PET scan (interval 21.7 ± 3.7 months), baseline...

Comparison of Gene-Transfer Efficiency in Human Embryonic Stem Cells

Technologies designed to allow manipulation and modification of human embryonic stem (hES) cells are numerous and vary in the complexity of their methods, efficiency, reliability, and safety. The most commonly studied and practiced of these methods include electroporation, lipofection, nucleofection, and lentiviral transduction. However, at present, it is unclear which protocol...

Development of a Three-Dimensional In Vitro Model for Longitudinal Observation of Cell Behavior: Monitoring by Magnetic Resonance Imaging and Optical Imaging

PurposeThe aim of this study is the development of a three-dimensional multicellular spheroid cell culture model for the longitudinal comparative and large-scale screening of cancer cell proliferation with noninvasive molecular imaging techniques under controlled and quantifiable conditions.ProceduresThe human glioblastoma cell line Gli36ΔEGFR was genetically modified to...

In Vivo Carbon-13 Dynamic MRS and MRSI of Normal and Fasted Rat Liver with Hyperpolarized 13C-Pyruvate

Background The use of in vivo 13C nuclear magnetic resonance spectroscopy in probing metabolic pathways to study normal metabolism and characterize disease physiology has been limited by its low sensitivity. However, recent technological advances have enabled greater than 50,000-fold enhancement of liquid-state polarization of metabolically active 13C substrates, allowing for...

On the Added Value of Baseline FDG-PET in Malignant Lymphoma

Purpose The added value of baseline positron emission tomography (PET) scans in therapy evaluation in malignant lymphoma is unclear. In guidelines, baseline PET is recommended but not mandatory except in lymphoma types with variable fluoro-d-glucose uptake. The aim of the present study was to test the hypothesis that adding baseline PET information decreases false positive...

Effective Inhibition of Xenografts of Hepatocellular Carcinoma (HepG2) by Rapamycin and Bevacizumab in an Intrahepatic Model

Purpose Hepatocellular carcinoma (HCC) displays a characteristic hypervascularity and depends on angiogenesis for tumor growth, which thus provides a potential target for therapeutic approaches to HCC. In this study, through the use of combined micro-positron emission tomography (PET)/computed tomography (CT), we investigate if such a combined targeting of vascular endothelial...

Evaluation of Tracer Kinetic Models for Analysis of [18F]FDDNP Studies

Purpose Different pharmacokinetic methods for [18F]FDDNP studies were evaluated using both simulations and clinical data. Procedures Methods included two-tissue reversible plasma (2T4k), simplified reference tissue input (SRTM), and a modified 2T4k models. The latter included an additional compartment for metabolites (2T1M). For plasma input models, binding potential, BPND, was...

First Evaluation of [11C]R116301 as an In Vivo Tracer of NK1 Receptors in Man

PurposeNK1 receptors have been implicated in various neuropsychiatric and other disorders. R116301 is a selective NK1 receptor antagonist. In this pilot study, [11C]R116301 was evaluated as a potential positron emission tomography (PET) ligand for the NK1 receptor.ProceduresTwo dynamic PET studies were performed in three normal volunteers before and after a blocking dose of...

[11C]-DPA-713 and [18F]-DPA-714 as New PET Tracers for TSPO: A Comparison with [11C]-(R)-PK11195 in a Rat Model of Herpes Encephalitis

BackgroundActivation of microglia cells plays an important role in neurological diseases. Positron emission tomography (PET) with [11C]-(R)-PK11195 has already been used to visualize activated microglia cells in neurological diseases. However, [11C]-(R)-PK11195 may not possess the required sensitivity to visualize mild neuroinflammation. In this study, we evaluated the PET...

A Randomised Controlled Trial Assessing the Effect of Oral Diazepam on 18F-FDG Uptake in the Neck and Upper Chest Region

ObjectiveA distinctive pattern of physiological symmetrical uptake of 18F-fluorodeoxyglucose (18F-FDG) in the neck and upper chest region is a phenomenon that is sometimes observed on positron emission tomography (PET) scans of some oncologic patients. Initially, it was assumed to be muscle uptake secondary to patient anxiety or tension, which could be prevented by diazepam...