Acta Physiologiae Plantarum

http://link.springer.com/journal/11738

List of Papers (Total 203)

Exogenous putrescine increases the responsiveness of thermodormant Avena fatua L. caryopses to karrikinolide and gibberellic acid

A natural feature of freshly harvested Avena fatua L. caryopses is primary dormancy which, however, was relieved partially by putrescine (Put) (10−2 M) and completely by karrikinolide (KAR1) (3 × 10−9 M) or gibberellic acid (GA3) (10−5 M). The sensitivity of A. fatua caryopses to these stimulators was adversely affected by supraoptimal temperature (SOT) (30 °C). A reduced ...

CaCl2 treatment improves drought stress tolerance in barley (Hordeum vulgare L.)

Spring drought can adversely affect the productivity of barley (Hordeum vulgare L.) by reducing the yield. Because seed osmopriming can enhance crop productivity, we examined the potential of CaCl2 treatment to improve drought tolerance in spring barley. Initially, we applied the priming procedure (5, 50, and 500 mM) to caryopses and assessed its effectiveness using a routine ...

Genetic transformation of gentian Gentiana tibetica (King) leaf explants with Agrobacterium tumefaciens strain C58C1

Experiments were carried out on transformation of leaf explants derived from the axenic culture of Gentiana tibetica (King) using a co-culture of Agrobacterium tumefaciens. A. tumefaciens octopine strain C58C1 carried neomycine phosphotransferase (nptII) and β-glucuronidase (uidA) genes. The influence of plasmid helper pCH32 on transformation efficiency was stressed. After ...

Plant-derived smoke induced activity of amylases, DNA replication and β-tubulin accumulation before radicle protrusion of dormant Avena fatua L. caryopses

Florets of Avena fatua (wild oat) did not germinate at temperatures from 5 to 35 °C, nor did caryopses at 5 and above 15 °C. Smoke–water (SW) was found to partly or substantially induce dormant florets and caryopses to germinate at temperatures from 10 to 25 °C, whereby almost all caryopses germinated at 15 and 20 °C. When florets were dry-stored at 25 °C for 4 months, their ...

Molecular cloning and functional analysis of the second gene encoding glutamate dehydrogenase in triticale

Glutamate dehydrogenase (GDH; EC 1.4.1.2) catalyzes the reductive amination of 2-oxoglutarate with ammonium and the oxidative deamination of glutamate. In some plants species, GDH is a hexamer and can be separated into seven isoenzymes that are composed of two distinct subunits: α and β. The large number of isoenzymes is the reason why GDH functions are still being intensively ...

Response of chloroplasts of tolerant and sensitive wheat genotypes to manganese excess: structural and biochemical properties

Effects related to the adaptation of wheat to Mn excess were determined by structural and biochemical characterization of chloroplasts obtained from three-leaf seedlings of Mn-treated wheat. Chloroplasts were isolated from two wheat genotypes: sensitive (Raweta) and tolerant (Parabola) cultivated in hydroponic conditions in Hoagland nutrient supplemented with 0 (control), 5, 10, ...

Identification and expression profiling of a novel Kunitz trypsin inhibitor (KTI) gene from turmeric, Curcuma longa, by real-time quantitative PCR (RT-qPCR)

Kunitz trypsin inhibitor (KTI) is one of the widely studied protease inhibitors (PIs) and has been reported to take part in plant defense mechanism during pathogenesis. Numerous plant-origin recombinant KTIs have been described to exhibit anti-pathogenic properties and were used to fight pathogens in the field of pharmacology and agriculture. In this study, a novel Kunitz trypsin ...

Photosynthesis and sucrose metabolism in leaves of Arabidopsis thaliana aos, ein4 and rcd1 mutants as affected by wounding

We have investigated the influence of mechanical wounding of Arabidopsis rosette leaves on photochemical activity of photosystem II, gas exchange, sugar content and sucrose metabolism in wild-type plants and mutants impaired in hormonal balance. The aos (jasmonate deficiency), rcd1 (reduced sensitivity to ABA, ethylene, and methyl-jasmonate), and ein4 (ethylene insensitivity) ...

Endogenous ABA content in relation to maturation of somatic embryos in Tulipa (L.) ‘Apeldoorn’ cultures

The aim of the present study was to estimate the endogenous abscisic acid (ABA) content in tulip ‘Apeldoorn’ torpedo and mature somatic embryos. Moreover, the effect of exogenous ABA and/or its inhibitor fluridone on somatic embryo maturation and conversion into plantlets was investigated. Torpedo-stage somatic embryos were subcultured on media containing 5 μM of picloram and 1 μM ...

Self-preserving lognormal volume-size distributions of starch granules in developing sweetpotatoes and modulation of their scale parameters by a starch synthase II (SSII)

Main conclusion Starch granule size distributions in plant tissues, when determined in high resolution and specified properly as a frequency function, could provide useful information on the granule formation and growth. Abstract To better understand genetic control of physical properties of starch granules, we attempted a new approach to analyze developmental and genotypic effects ...

Water relations in plants subjected to heavy metal stresses

Concentrations of heavy metals in soil seldom reach a level sufficient to cause osmotic disturbances in plants. It is likely that water entry to the roots is indirectly governed by other factors which are themselves affected by metals. Decreased elongation of the primary root, impaired secondary growth, increased root dieback, or reduced root hair caused by toxic ions all exert a ...

Morphogenesis of sporotrophophyll leaves in Platycerium bifurcatum depends on the red/far-red ratio in the light spectrum

Although the fern Platycerium bifurcatum is popular among the producers of ornamental plants, it is a species of relatively poorly known physiology. Studies concerning the impact of the red/far-red (R/FR) ratio on photomorphogenesis refer primarily to spermatophytes. However, quite different phytochrome response mechanism functions in ferns, involving the localization of ...

Sirtuins: not only animal proteins

Sirtuins are proteins belonging to the group of NADH-dependent deacetylase and mono-ADP-ribosyltransferase enzymes. Sirtuins have been discovered for the first time in yeasts, subsequent studies have shown their presence in bacteria, plants and animals. These enzymes are frequently called longevity enzymes due to the fact that they are part of genetic apparatus involved in aging ...

Methyl jasmonate-dependent senescence of cotyledons in Ipomoea nil

Jasmonic acid methyl ester (JAMe) has been recently shown to play a crucial role in many physiological processes. In this paper, we focused on cotyledon senescence in Ipomoea nil and revealed that JAMe and darkness are the main factors stimulating the process examined. What is more, we showed that mefenamic acid (a jasmonate biosynthesis inhibitor) reverses the stimulatory effect ...

Application of the effective formula of growth functional to quantitative description of growth of plant cells

An effective formula describing expansive plant growth is derived from the modified Lockhart/Ortega-type equation. Its applicability is demonstrated on selected experimental data extracted from available literature. Quantitative information about the “diffusion rate” (k 2) of the growth factors is obtained for two different model species in plant science: Arabidopsis thaliana L. ...

Metabolic response of cornflower (Centaurea cyanus L.) exposed to tribenuron-methyl: one of the active substances of sulfonylurea herbicides

Tribenuron-methyl is the active substance of the herbicide used for weed control in crops. The aim of this study was to investigate differences in the metabolic response of seeds, seedlings and leaves of Centaurea cyanus L., depending on the degree of resistance to tribenuron-methyl. Changes in the values of selected biochemical and physiological parameters (germination index, ...

Analysis of elements and bacosides in in vitro shoot culture of Bacopa monnieri

The experiments on Bacopa monnieri from Scrophulariaceae family were performed in in vitro shoot cultures conditions in media enriched with Mg ions and serine or anthranilic acid (L1 vitamin). As there is limited literature data available on the content of elements in shoots from B. monnieri in vitro cultures, analyses of magnesium, zinc, potassium, calcium, copper, sodium and iron ...

Do the seeds of Solidago gigantea Aiton have physiological determinants of invasiveness?

Solidago gigantea Ait. (goldenrod) belongs to the most expansive environmental weeds, and it is the most dangerous plant-invader of American origin in Europe. The species easily propagates vegetatively, but it also produces large amounts of wind-disseminated achenes that contribute to the colonization of new areas. A sound knowledge of the germination biology of goldenrods is ...

Exogenous melatonin expediently modifies proteome of maize (Zea mays L.) embryo during seed germination

Melatonin (N-acetyl-5-methoxytryptamine) has a great potential for plant biostimulation. Its role in plant physiology is intensively explored, and its important function in plant stress defence frequently underlined. Melatonin is particularly effective when applied as an additional factor of seed priming. In the presented research, hydroconditioning was chosen experimentally as ...

Changes in the OXPHOS system in leaf and root mitochondria of Arabidopsis thaliana subjected to long-term sulphur deficiency

Long-term sulphur (S) deficiency in Arabidopsis thaliana affects the functioning of the mitochondrial oxidative phosphorylation system (OXPHOS) via alteration of the multisubunit NADH-ubiquinone oxidoreductase (Complex I; EC 1.6.5.3), which contains several iron–sulphur clusters. Densitometric analysis of bands of respiratory chain complexes after one-dimensional blue-native ...

The AtDREB1A transcription factor up-regulates expression of a vernalization pathway gene, GmVRN1-like, delaying flowering in soybean

The dehydration-responsive element binding proteins/C-repeat binding factors (i.e., DREBs/CRT) are crucial transcription factors, they can bind the cis-elements containing the A/GCCGAC sequence in the promoter region. Overexpression of DREB genes enhances the resistance to multiple abiotic stresses, but also causes dwarfism and delayed flowering in many plant species. In this ...

Compartment-specific investigations of antioxidants and hydrogen peroxide in leaves of Arabidopsis thaliana during dark-induced senescence

The aim of this study was to gain insight into the compartment-specific roles of ascorbate and glutathione in leaf senescence in Arabidopsis thaliana. The subcellular distribution of ascorbate, glutathione, and hydrogen peroxide (H2O2) was analyzed by transmission electron microscopy and correlated with the activity of antioxidative enzymes in wildtype plants and the ascorbate- and ...