Tuberculosis co-infection and its associated factors among People living with HIV/AIDS attending antiretroviral therapy clinic in southern Ethiopia: a facility based retrospective study

BMC Research Notes, Jun 2018

Objective The study aimed to determine the prevalence and identify determinants of TB among People living with HIV/AIDS (PLWHAs) through reviewing and analyzing patient case files from the anti-retro viral treatment (ART) clinic of Yirgalem General Hospital, southern Ethiopia. Results Of the total PLWHAs involved in the study, 51 (36.9%) of them were found to have TB, and of which, 37 (72.5%) were smear negative cases. The multivariate analysis showed that PLWHA’s who are at WHO clinical stage 3 (AOR = 5.82; 95% CI 1.04–32.30), CD4 level of 200–500 cells/mm3 (AOR = 4.85; 95% CI 1.95–12.05) and < 200 cells/mm3 (AOR = 7.34; 95% CI 2.75–19.58) at ART initiation, and who didn’t take INH prophylaxis (AOR = 12.36; 95% CI 4.47–34.14) were significantly associated with TB-HIV co-infection. Rapid and sensitive diagnostic techniques should be implemented to early detect co-infections, and also INH prophylactic preventive measures should be strengthened to reduce TB incidence.

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Tuberculosis co-infection and its associated factors among People living with HIV/AIDS attending antiretroviral therapy clinic in southern Ethiopia: a facility based retrospective study

BMC Research Notes December 2018, 11:417 | Cite as Tuberculosis co-infection and its associated factors among People living with HIV/AIDS attending antiretroviral therapy clinic in southern Ethiopia: a facility based retrospective study AuthorsAuthors and affiliations Abel NegussieDamene DebalkeTeshome BelachewFetlework Tadesse Open Access Research note First Online: 28 June 2018 Received: 04 February 2018 Accepted: 23 June 2018 157 Downloads Abstract Objective The study aimed to determine the prevalence and identify determinants of TB among People living with HIV/AIDS (PLWHAs) through reviewing and analyzing patient case files from the anti-retro viral treatment (ART) clinic of Yirgalem General Hospital, southern Ethiopia. Results Of the total PLWHAs involved in the study, 51 (36.9%) of them were found to have TB, and of which, 37 (72.5%) were smear negative cases. The multivariate analysis showed that PLWHA’s who are at WHO clinical stage 3 (AOR = 5.82; 95% CI 1.04–32.30), CD4 level of 200–500 cells/mm3 (AOR = 4.85; 95% CI 1.95–12.05) and < 200 cells/mm3 (AOR = 7.34; 95% CI 2.75–19.58) at ART initiation, and who didn’t take INH prophylaxis (AOR = 12.36; 95% CI 4.47–34.14) were significantly associated with TB-HIV co-infection. Rapid and sensitive diagnostic techniques should be implemented to early detect co-infections, and also INH prophylactic preventive measures should be strengthened to reduce TB incidence. KeywordsTuberculosis HIV/AIDS Co-infection PLWHA Southern Ethiopia  Abbreviations AFB acid fast Bacilli AOR adjusted odds ratio ART anti-retro viral treatment EPTB extra-pulmonary tuberculosis MTB Mycobacterium tuberculosis PLWHA People living with HIV/AIDS PTB pulmonary tuberculosis TB tuberculosis WHO World Health organization Electronic supplementary material The online version of this article ( https://doi.org/10.1186/s13104-018-3530-3) contains supplementary material, which is available to authorized users. Introduction Tuberculosis (TB) remains a major public health problem in resource-limited countries and it is the largest cause of death among People living with HIV/AIDS (PLWHAs) worldwide. According to the WHO Global TB Report, there were an estimated 9 million new cases of TB occurred worldwide, of which 13% were associated with HIV/AIDS infection, and 78% of these HIV/AIDS associated cases occurred in Africa. It is further estimated that 1.49 million deaths from TB, including 0.36 million among PLWHAs, and 96% of the deaths occurred in developing countries. Ethiopia has been classified 7th among the 22 high burden countries with TB and HIV/AIDS infection in the world [1, 2]. HIV/AIDS predisposes for Mycobacterium tuberculosis (MTB) infection and increases the probability of recently acquired TB infection to progress to the status of active disease and increases the rate of recurrent TB. The lifetime risk of developing active TB in immune-competent adults is estimated to be 10%, but in PLWHAs who infected with MTB the annual risk of developing active TB disease exceeds 10% [3]. The current increasing HIV/AIDS associated tuberculosis shifted the clinical pattern of TB towards smears negative pulmonary TB (PTB) and extra-pulmonary TB (EPTB), which in turn, causes difficulties in the diagnosis and treatment of TB due to unusual clinical picture with increased smear negative acid fast Bacilli (AFB) PTB, atypical finding on chest radiography and increased prevalence of EPTB [4, 5]. Several studies documented that the clinical manifestations of TB in PLWHAs are quite varied and generally show different patterns as a function of the CD4+ T cell count. In addition, TB can appear at any stage of HIV/AIDS infection, and its presentation varies with the WHO stage and CD4+ lymphocyte count, although it is more frequent at CD4+ T cell count of < 300 cells/mm3 [6, 7]. In the study area, there is no adequate and recent data on the prevalence and determinants of TB in PLWHAs. Even though it is expected that the prevalence of TB among PLWHAs in the study area is assumed to be higher than other sites in the region due to the large number of PLWHAs treated in the ART clinic, this expectation should be supported by area specific study. The objective of the study was to determine the prevalence of TB and its determinant factors among PLWHAs in a local context that will help to reduce the burden of the disease by facilitating the early detection of at-risk patients and to serve as a reference document for further studies. Main text Methods The study was conducted in Yirgalem General Hospital, which is found in Yirgalem town, Sidama Zone, southern Ethiopia. It serves around 4.2 million populations of Sidama Zone and part of southern Oromia region. A total of 5944 PLWHAs were registered since the ART clinic in the hospital had started its function in 2001. The trend of HIV/AIDS cases has decreased over time and the highest number of cases was registered in 2005/2006 with a total of 1219 cases. A facility-based retrospective cross-sectional study was carried out to determine the prevalence of TB and identify its determinant factors among PLWHAs through reviewing and analyzing a 2 year (from January 1, 2015 to December 30, 2016) patient case files from the ART clinic of Yirgalem General Hospital. All PLWHAs attending the ART clinic of Yirgalem General Hospital during the study period were eligible for the study, and a total of 182 PLWHAs were diagnosed and treated during the period. Taking the estimated proportion (P) of TB among PLWHAs = 0.1 [1], margin of error = 5% and confidence level = 95%; the calculated sample size became 138. The 2 years HIV/AIDS patients’ case files were identified and the study participants were selected by lottery method after excluding patients with incomplete medical records. Data was collected from patients’ case files using a checklist which was prepared by adopting previous studies [6, 7, 8]. The checklist was pre-tested before the actual data collection and some variables like adherence to ART medications and nutritional status were omitted because of incomplete medical records. Otherwise, the checklist was inclusive of the different variables (age, sex, occupation, residence, INH prophylaxis, CD4 count, ART status) which were assumed as possible determinants of TB occurrence among PLWHAs. The data were entered, cleaned and analyzed using SPSS version 19 statistical software. Frequencies and percentages were calculated for descriptive analysis of socio-demographic, clinical and laboratory profile of the study participants. Binary and multiple logistic regression analyses were applied to determine associations and identify risk factors of TB occurrence among PLWHAs taking P ≤ 0.05 for statistical significance. Results A total of 138 PLWHA’s medical records were reviewed and most of the participants were females (57.9%), aged 16–45 years (83.3%), urban residents (56.5%) and Protestants (52.2%) by religion. Moreover, 65 (48.9%) of the study participants were married and 73 (54.9%) of them attended primary school (Additional file 1: Table S1). Of the total PLWHAs involved in the study, 51 (36.9%) of them were found to have TB; and of the 51 with TB, 37 (72.5%) were smear negative cases (Fig. 1). In addition, 37 (72.5%), 9 (17.6%) and 3 (5.9%) of the TB positive patients were diagnosed by chest x-ray, microscopy (AFB) and biopsy, respectively. Open image in new window Fig. 1 Types of TB among PLWHAs, Yirgalem General Hospital, southern Ethiopia; January 2015 to December 2016. Open image in new window TB category Out of the total selected PLWHAs, 47 (34%) of them didn’t take INH prophylaxis; and among them, 35 (74.5%) were TB positive patients. Furthermore, 126 (91.3%) of the study participants had started ART, and of them 61 (48.4%) had CD4 level of < 200 cells/mm3 at ART initiation (Table 1). Table 1 Clinical and laboratory profiles of study participants in Yirgalem General Hospital, southern Ethiopia; January 2015 to December 2016 Variables Category Frequency Percent TB diagnosis Positive 51 37.0 Negative 87 63.0 Total 138 100 INH prophylaxis Yes 91 65.9 No 47 34.1 Total 138 100 WHO stage Stage 1 66 47.8 Stage 2 30 21.7 Stage 3 29 21.0 Stage 4 13 9.4 Total 138 100 ART status Pre-ART 12 8.7 ART 126 91.3 Total 138 100 CD4 level at ART initiation > 500 21 16.7 200–500 44 34.9 < 200 61 48.4 Total 126 100 CD4 level during diagnosis > 500 27 19.9 200–500 49 36.0 < 200 60 44.1 Total 136 100 The bivariate analysis result showed that residence, INH prophylaxis status, WHO clinical stage and CD4 level at ART initiation were associated with TB-HIV/AIDS co-infection. In the multivariate analysis, multiple logistic regression was used to identify determinants of TB occurrence among PLWHAs. Being at WHO clinical stage 3 (AOR = 5.82; 95% CI 1.04–32.30), not taking INH prophylaxis (AOR = 12.36; 95% CI 4.47–34.14), CD4 level of < 200 cells/mm3 (AOR = 7.34; 95% CI 2.75–19.58) and a CD4 level of 200–500 cells/mm3 (AOR = 4.85; 95% CI 1.95–12.05) at ART initiation were significant determinants of increased risk of TB co-infection among PLWHAs (Table 2). Table 2 Determinant factors of TB-HIV co-infection among PLWHAs, Yirgalem General Hospital, southern Ethiopia, 2017 Variables TB disease COR (95% CI) AOR (95% CI) P value Yes No Age (years)  < 15 2 3 1.12 (1.16–9.01)     16–45 41 74 1.44 (0.52–9.94)     ≥ 46 8 10 1    Sex  Male 21 37 1.05 (0.52–2.13)     Female 30 50 1    Residence  Urban 24 54 1 1    Rural 27 33 0.54 (0.27–1.09) 0.65 (0.23–1.77) 0.40 Occupation  Housewife 18 31 1     Government employee 4 8 1.16 (0.30–4.40)     Merchant 9 12 0.77 (0.27–2.19)     Farmer 6 11 1.06 (0.33–3.36)     Student 5 4 0.46 (0.11–1.95)     Daily laborer 3 2 0.38 (0.05–2.54)     Unemployed 3 1 1.19 (0.01–2.00)    Marital status  Married 26 47 1     Single 10 17 0.94 (0.37–2.35)     Divorced 11 15 0.75 (0.30–1.88)     Widowed 3 4 0.73 (0.5–3.55)    INH prophylaxis  Yes 16 75 1 1    No 35 12 13.67 (5.84–31.96) 12.36 (4.47–34.14) < 0.01* ART profile  Pre-ART 3 9 1.84 (0.47–7.15)     ART 48 78 1    WHO stage  Stage 1 0 66 1 1    Stage 2 10 20 0.61 (0.15–2.42) 0.74 (0.12–4.32) 0.16  Stage 3 21 8 7.20 (2.00–25.90) 5.82 (1.04–32.30) 0.002*  Stage 4 8 5 3.20 (0.82–12.35) 2.10 (0.35–12.37) 0.06 CD4 level at ART initiation  > 500 5 16 1 1    200–500 13 31 2.30 (1.01–5.23) 4.85 (1.95–12.05) < 0.01*  < 200 30 31 3.09 (1.00–9.51) 7.34 (2.75–19.58) < 0.01* * Statistically significant at P < 0.05 Discussion Tuberculosis is a well-recognized opportunistic infection in patients with HIV/AIDS. In our study, we found that TB-HIV/AIDS co-infection was 36.9% and it was higher than the findings of other studies [5, 8, 9, 10]. This variation in the magnitude of tuberculosis in PLWHAs may be due to high TB detection rate as a result of availability of TB-diagnostic facilities or due to differences in HIV/AIDS infection rate at the population level. The other relevant finding is that 72.5% of the TB-HIV/AIDS co-infected cases were smear-negative and this result is higher than the findings of other studies conducted elsewhere [7, 8]. This higher smear negativity indicates the importance of TB diagnostic techniques, other than AFB microscopy. In the study area, clinical data is supplemented with chest radiography to diagnose suspicious smear negative cases. INH prophylaxis status, being at WHO clinical stage of 3, having a CD4 level of 200–500 and < 200 cells/mm3 at ART initiation were identified as risk factors of TB occurrence among PLWHAs. These findings are in line with other Hospital-based studies in Ethiopia [7, 8]. Our study revealed a poor INH prophylactic management for PLWHAs. But, the role of INH prophylaxis in the reduction of TB incidence has been reported in many clinical trials and found to be important [3]. The most important determinant factor for development of TB in PLWHAs is the immunologic state of the person and maintaining the CD4+ lymphocyte cell count level as high as possible helps the person to have a low risk of infection. Conclusions In our study, the prevalence of TB and the proportion of smear negative TB among PLWHAs was high. The risk of TB occurrence was found to be high among PLWHAs with WHO clinical stage 3, low CD4 level at ART initiation and PLWHAs who didn’t receive INH prophylaxis. The high proportion of smear negative TB cases among PLWHAs requires special attention for confirming clinically suspected TB cases; and in addition to clinical and radiological investigations, rapid and sensitive diagnostic techniques like Culture, Fluorescent Microscopy and Xpert MTB/RIF should be implemented by Yirgalem General Hospital. Furthermore, our study revealed a poor INH prophylactic management for PLWHAs, and therefore, INH prophylactic preventive measures should be strengthened in the Hospital to reduce TB incidence among PLWHAs. Limitations Despite the study’s endeavor of identifying determinants of TB infection among PLWHAs, as the study is a facility based study undertaken in a single hospital with limited number of patients, it is difficult to make generalizations and future community-based studies are needed to substantiate the study findings. Notes Authors’ contributions Conceived and designed the study: AN, TB, DD, FT. Performed the data collection: TB, DD, FT. Analysed the data: AN, TB, DD, FT. Supervised the conduct of the study: AN. Wrote the manuscript: AN. All authors read and approved the final manuscript. Acknowledgements We would like to acknowledge Yirgalem Hospital Medical College for funding this research. We are grateful to Yirgalem General Hospital and ART clinic staffs for their support and cooperation. Competing interests The authors declare that they have no competing interests. Availability of data and materials The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request. Consent for publication Not applicable. Ethics approval and consent to participate The research protocol was approved by the Ethical Review Committee of Yirgalem Hospital Medical College and prior permission to collect data was obtained from Yirgalem General Hospital. The study subjects were identified using codes and no identifier information was recorded to ensure confidentiality. Funding This study was funded by Yirgalem Hospital Medical College, Ethiopia. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher’s Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Supplementary material 13104_2018_3530_MOESM1_ESM.doc (52 kb) Additional file 1: Table S1. Socio-demographic profile of study participants in Yirgalem General Hospital, southern Ethiopia; January 2015 to December 2016. References 1. Federal Ministry of Health (Ethiopia). Guidelines for clinical and programmatic management of TB, TB/HIV/AIDS and leprosy in Ethiopia. 5th ed. Addis Ababa: Federal Ministry of Health; 2013.Google Scholar 2. World Health Organization. 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BMC Res Notes. 2015;8:666.CrossRefPubMedPubMedCentralGoogle Scholar Copyright information © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Authors and Affiliations Abel Negussie1Email authorView author's OrcID profileDamene Debalke1Teshome Belachew1Fetlework Tadesse21.Yirgalem Hospital Medical CollegeYirgalemEthiopia2.Menelik II Referral HospitalAddis AbabaEthiopia


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Abel Negussie, Damene Debalke, Teshome Belachew, Fetlework Tadesse. Tuberculosis co-infection and its associated factors among People living with HIV/AIDS attending antiretroviral therapy clinic in southern Ethiopia: a facility based retrospective study, BMC Research Notes, 2018, 417, DOI: 10.1186/s13104-018-3530-3