A novel fluorescent marker for molecular, structural and functional analysis of the cardiac conduction system

Tijdschrift voor Kindergeneeskunde, May 2013

Introduction The function of the conduction system depends on structure, function and molecular composition of its components. Studying the relation between these aspects would greatly benefit from a mouse model in which the cells of the conduction system can be easily recognized in situ. Methods We generated a transgenic mouse model that expresses yellow fluorescent protein Venus under control of the Tbx3 locus (Tbx3Venus). Results The expression pattern of Venus precisely recapitulates that of Tbx3, and specifically marks the conduction system components, including the sinus node, but excluding the Purkinje fiber network. Combined optical mapping and fluorescence microscopy demonstrated that the cardiac impulse always originated from the fluorescently labeled sinus node region. Furthermore, patch-clamp of fluorescently labeled cells isolated from embryos, fetuses and adults revealed that these cells display sinus node action potentials and spontaneous depolarization. These data indicate t ...

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A novel fluorescent marker for molecular, structural and functional analysis of the cardiac conduction system

Martijn L. Bakker 0 Bas J. Boukens 0 Vincent Wakker 0 Arie O. Verkerk 0 Corrie de Gier-de Vries 0 Malou van den Boogaard 0 Antoon F. Moorman 0 Vincent M. Christoffels 0 0 Department of anatomy , embryology and fysiology, AMC - We generated a transgenic mouse model that expresses yellow fluorescent protein Venus under control of the Tbx3 locus (Tbx3Venus). We conclude that Tbx3Venus is a reliable tool to visualize the conduction system cells in situ, to isolate pacemaker cells and to facilitate studies integrating structure, function and molecular composition of the conduction system during development and disease.


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Martijn L. Bakker, Bas J. Boukens, Vincent Wakker, Arie O. Verkerk, Corrie de Gier-de Vries, Malou van den Boogaard, Antoon F. Moorman, Vincent M. Christoffels. A novel fluorescent marker for molecular, structural and functional analysis of the cardiac conduction system, Tijdschrift voor Kindergeneeskunde, 2013, 19, DOI: 10.1007/s12456-013-0019-9