Freshwater bryozoans of Lithuania (Bryozoa)

ZooKeys, Aug 2018

Nine species of freshwater bryozoans were recorded in Lithuania in a survey of 18 various types of freshwater bodies. Eight species were assigned to the Class Phylactolaemata and families Plumatellidae and Cristatellidae (Plumatella repens, Plumatella fungosa, Plumatella fruticosa, Plumatella casmiana, Plumatella emarginata, Plumatella geimermassardi, Hyalinella punctata and Cristatella mucedo). The ninth species, Paludicella articulata, represented the Class Gymnolaemata. Plumatella geimermassardi and P. casmiana were recorded for the first time in Lithuania. For the plumatellids, species identification was achieved partly by analysing statoblasts’ morphological ultrastructures by scanning electron microscopy.

A PDF file should load here. If you do not see its contents the file may be temporarily unavailable at the journal website or you do not have a PDF plug-in installed and enabled in your browser.

Alternatively, you can download the file locally and open with any standalone PDF reader:

Freshwater bryozoans of Lithuania (Bryozoa)

ZooKeys Freshwater bryozoans of Lithuania (Bryozoa) Ingrida Šatkauskienė 0 Timothy Wood Jurgita Rutkauskaitė-Sucilienė 0 Vida Mildažienė 0 Simona Tučkutė 0 Vytautas Magnus University, Faculty of Natural Sciences, Department of Biology , Vileikos str. 8-802, 44404 Kaunas , Lithuania 2 Wright State University, Department of Biological Sciences , Dayton, OH 45435 , USA 3 Lithuanian Energy Institute , 3 Breslaujos str., LT-44403, Kaunas , Lithuania Nine species of freshwater bryozoans were recorded in Lithuania in a survey of 18 various types of freshwater bodies. Eight species were assigned to the Class Phylactolaemata and families Plumatellidae and Cristatellidae (Plumatella repens, Plumatella fungosa, Plumatella fruticosa, Plumatella casmiana, Plumatella emarginata, Plumatella geimermassardi, Hyalinella punctata and Cristatella mucedo). The ninth species, Paludicella articulata, represented the Class Gymnolaemata. Plumatella geimermassardi and P. casmiana were recorded for the first time in Lithuania. For the plumatellids, species identification was achieved partly by analysing statoblasts' morphological ultrastructures by scanning electron microscopy. eol>Phylactolaemata; Plumatella; statoblasts; bryozoa; Lithuania - A peer-reviewed open-access journal 53 Launched to accelerate biodiversity research Introduction Freshwater bryozoans grow in colonies of minute tentacle-bearing clones (zooids) that feed upon microscopic plankton. They are often found in ponds, lakes, and rivers, forming a cryptic but often a significant part of the aquatic fauna (Bushnell 1966) . Bryozoans are important to ecosystems as filter feeders (Wood et al. 2006) , extracting phytoplankton from the water and producing faecal pellets that nourish benthic meiCopyright Ingrida Šatkauskiene˙ et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ofauna (Bushnell and Rao 1974) . The colony structure also creates important habitat and shelter for other organisms: protozoans, rotifers, ostracods, nematodes and chironomids (Ricciardi and Reiswig 1994) . Bryozoans hosting certain myxozoan parasites can spread proliferative kidney disease in fish, which is often fatal in farmed and wild fish populations ( Grabner and El-Matbouli 2008 ; Bartošová-Sojková et al. 2014 ). In their natural habitat freshwater bryozoans are easily overlooked and, in many areas, there is little information on the identity or distribution of species. Fundamental studies of freshwater bryozoans in Europe were launched with a pioneering monograph by Allman (1856) , which established the Class Phylactolaemata as an exclusively freshwater group and named Plumatellidae as the largest family within that class. A second monograph by Jullien (1885) described species from France following Allman’s taxonomy. Shortly afterwards a third monograph appeared from Germany (Kraepelin 1887) proposing a new taxonomic scheme for the plumatellids which has by now fallen out of use. Since those early years there have been bryozoan surveys from a number of European countries, including the Netherlands (Lacourt 1949) , Sweden (Borg 1941) , Italy ( Viganó 1965 , Luxembourg (Geimer and Massard 1986) , Belgium (Loppens 1906) , Bulgaria (Gruncharova 1968) , Ireland (Smyth 1994) , and Britain (Mundy 1980) . In most of these works only 5–8 species were documented. Until recently freshwater bryozoans in the Baltic region were known only from a brief paper from Latvia (Trauberg 1940) . Six species were listed using the Kraepelin taxonomic scheme. They included Plumatella polymorpha Krpln. var. repens (L.), Krpln., Plumatella polymorpha Krpln. var. appressa Krpln., Plumatella polymorpha Krpln. var. fungosa (Pall.) Krpln., Plumatella princeps Krpln. var. emarginata Allm., Cristatella mucedo Cuv., and Paludicella ehrenbergi Bened. Trauberg (1940) provided measurements of certain colonies and statoblasts and included some ecological information as well. In 2015 an old master’s thesis was uncovered in Lithuania with a detailed account of bryozoans from the area (Satkauskiene et al. 2018) . Written by Bronė Pajiedaitė (1933) the work covered a period of 1931–1933. Pajiedaitė collected bryozoans from widely scattered locations in Lithuania (five districts and approximately eleven localities) including lakes, ponds, and rivers. From microscopic examinations, she illustrated colonies and statoblasts, prepared notes on associations with other organisms, and described substrata on which common bryozoans were found. During her studies, Bronė Pajiedaitė prepared at least 12 bottles of fixed whole specimens and 70 high quality microscope slides (Satkauskiene et al. 2018) . Unfortunately, the whole specimens were apparently destroyed during World War II (1941–1945), but the surviving microscopic slides are now deposited in Vilnius University. Altogether Pajiedaitė described seven species of freshwater bryozoans in detail: Paludicella articulata (Ehrenberg, 1831) , Cristatella mucedo (Cuvier, 1798) , Plumatella fungosa (Pallas, 1768) , Plumatella repens (Linnaeus, 1758) , Plumatella emarginata (Allman, 1844) , Plumatella fruticosa (Allman, 1844) , and Hyalinella punctata (Hancock, 1850) . In recent years the number of freshwater bryozoans documented from Europe has grown to 19 (Massard and Geimer 2005, 2008a; Wood and Okamura 2004) . Meanwhile from seven to eleven freshwater bryozoan species have been reported in countries neighbouring Lithuania: Latvia, Poland, and Belarus (Kaminski 1984, Fauna Europea 2013) . Based on the species diversity in other European countries, we can expect a more diverse list of bryozoans in Lithuania as well. The present work describes freshwater bryozoans studied in 18 freshwater bodies in Lithuania. Materials and methods Climate of Lithuania Lithuania is distinguished by a highly diverse geography: plains, hills, abundant forests, lakes, wetlands, and Baltic Sea. The climate of the Lithuania can be described as typical European with strong continental influence providing warm summers and fairly severe winters. The weather is often windy and humid due to the proximity of the Baltic Sea. The average air temperature is 7.2 °C. July is the warmest month with an average temperature of 18 °C. January and February are the coldest months with average temperatures around -3.35 °C, but sometimes winter days can be much colder with temperatures about -32.4 °C. Annual precipitation ranges from 560 to 700 mm. Snow cover can last from 60 to 90 days. The flat landscape retains much of the precipitation, which leads to a relatively high water level (Lithuanian Hydrometeorological Service under the Ministry of Environment). Characteristic of sampling sites Our bryozoan survey was conducted during April through October 2015–2017. We investigated localities that included different types of water bodies: lakes, ponds, lagoons and lotic habitats (streams and rivers). Figure 1 shows regions in Lithuania that were surveyed. Geographical details and descriptions of collecting sites are listed below and summarized in Table 1. Region 1 Pond in Kaunas botanical garden (located in Kaunas city). Small eutrophic pond with abundant macro- and microalgae. The bottom is sludge. Water pH is 7.89. Linksmakalnis pond (Kaunas district). Large artificial pond, what shorelines are overgrown by Phragmites sp. The bottom is sandy. Water pH is 7.18. Raudondvaris, Rokai and Tribalė ponds. All these ponds located in Kaunas district and have similar characteristics: the bottom is sand mixed with sludge, the littoral is overgrown by Acorus calamus and Phragmites sp. in Raudondvaris and Tribalė ponds. Vegetation on the shores of Rokai pond are rare, water birds are common here. Water pH varies from 7.51–7.85 (Rokai and Tribalė ponds respectively) to pH 8.35 in Raudondvaris pond. Lampėdžiai lake (located in Kaunas city). Relative large (1,252 km²), semi-artificial lake. The bottom is sandy. Shorelines are without the trees, only Phragmites sp. occurs occasionally in the littoral. Water pH is 8.06. Maišia stream (located in the outskirts of Kaunas). One side of shore is overgrown by deciduous trees, Phragmites sp. and Typha angustipholia. Water is polluted by sewage. Water pH is 7.48. Veršvio stream (located in western part of Kaunas city). Small and shallow stream, that dries up in the summer. Shore is lined by trees and shrubs. Bottom is sandy. Water pH is 7.79. Region 2 Šventupė pond (Ukmergė district). The shoreline is overgrown by shrubs and other vegetation. Phragmites sp., Acorus calamus, Lemna minor dominates in littoral. A small stream enters in one end of pond. Another end of the pond is connected with Šventoji River. Water pH 7.40. Mūša pond (Ukmergė district). Mūšia stream enters in this artificial pond. Phragmites sp., Acorus calamus and Nymphaea lutea occur in the littoral. Water pH is 7.81. Region 3 Plateliai Lake (Plungė district) is the large lake covering about 12 km² with a maximum depth of 47 m. Water is contributed by seventeen small streams. The Bottom is sandy in the collecting sites. Skyplaičiai Lake (Plungė district) covers 0.068 km² and is surrounded by a mixed deciduous forest. The bottom is muddy; shorelines are overgrown by Phragmites sp. According to the EU Habitats Directive, this lake is notable for its Charophyta communities. Region 4 Region 5 Region 6 Škilietai Lake (Trakai district) covers about 0.033 km² with maximum depth of 12 m. The lake is surrounded by pine forest. Elektrėnai Reservoir (Vilnius district and Trakai district) is the third largest artificial lake in Lithuania. The reservoir measures about 0.0126 km². The lake is fed by inflows from the Strėva River, and nine other rivulets. Strėva River (Trakai and Kaunas district). Average current velocity is 0.1–0.3 m/s. The bottom is sandy mixed with silt. Phragmites sp. and Nymphaea lutea grow at the edges of the river. Water pH was 7.80 in the sampling site. Saterečius Pond (Utena district). The pond is surrounded by marsh and mixed deciduous forest dominated by Alnus sp. In summer the pond is almost overgrown with macro-algae and such macrophytes as Nymphaea lutea and Stratiotes aloides. Water pH is 6.78. Žvirgždelis Lake (Utena district) covers an area of 0.027 km²; the bottom is silt and Phragmites sp. dominates in littoral. Water pH is 7.03. Snaigynas Lake (Lazdijai district). The lake covers an area of 2 km², with an average depth of 3 m. The shores are low and overgrown by shrubs and trees. The lake bottom is sandy in littoral. A small shallow stream flows out from this lake into Trikojis Lake. Sampling and observations Statoblasts were taken by net from the surface of water and aquatic plants. Bryozoan colonies were collected from submerged branches, stones, and aquatic plants in the littoral of the water bodies. Statoblasts and bryozoan colonies were stored in 70% ethanol. Identification of most species was based on morphology of statoblasts and colony (when colonies were available) using light and scanning electron microscopy (SEM) (Hitachi S-3400N). Statoblasts characters included overall length and width, length and width of the fenestrae, and surface micro-sculpture of statoblasts. Abbreviations used for measurements are as follows: ratio of the statoblast, ventral fenestra length; ventral fenestra width; DfL DfW dorsal fenestra length; dorsal fenestra width. Measurements were taken from SEM images with software Original Hitachi S3400N Scanning Electron Microscope software ver 7.3. Statoblasts were rinsed with distilled water several times then treated by KOH in order to remove any debris and cleaned using vortex for a few minutes. Statoblasts were prepared for scanning electron microscopy by simple drying without sputtering. The identification keys by Wood and Okamura (2005) were used. Material examined The authors collected specimens during April through October 2015–2017. In total, 53 statoblasts and 8 colonies collected from 18 localities in Lithuania were examined. In addition, some data collected by Bronė Pajiedaitė (1932-1934) were included in this study for comparison. The representative specimens are deposited in the zoological collection of Biology Department of Vytautas Magnus University. Results The survey of 18 water bodies yielded nine species of freshwater bryozoans (Table 1). Eight of these are classified with the Class Phylactolaemata: Cristatella mucedo, Hyalinella punctata, Plumatella casmiana Oka, 1907 , Plumatella fungosa, Plumatella fruticosa, Plumatella geimermassardi Wood & Okamura, 2004 Plumatella repens, and Plumatella emarginata. The ninth species, Paludicella articulata, belongs to the Class Gymnolaemata. Taxonomy Class Phylactolaemata Allman, 1856 Order Plumatellida Allman, 1856 Family Plumatellidae Allman, 1856 Plumatella repens (Linnaeus, 1758) Fig. 2 Material examined. Ten floatoblasts collected from ponds of Kaunas Botanical garden, Raudondvaris pond in April 2015, and Skyplaičiai lake, collected in June 2015; colonies collected from Raudondvaris and Rokai pond in June 2015 and July 2016 respectively. Sessoblasts were not found. Description. Colonies were about 5–8 cm size. The transparent branches of colonies were attached to the substratum for almost whole of their length. Floatoblasts were identified by the broadly oval shape and the absence of tubercles on the statoblast annulus (Fig. 2). Floatoblasts were 315–341 (325±3) μm long by 226–270 (252±4) μm wide; L/W ratio was 1.3; VfL 144–245 (187±14) μm; VfW 126–212 (168±10) μm(n=10); DfL 135–258 (178±14) μm; DfW126–212 (163± 9) μm (n=10). Fenestra of floatoblasts circular, covered with rounded tubercles that become less prominent towards the centre of fenestra. The annular nodules often described for this species have not yet been observed in Lithuanian material. Distribution in Europe. According to Økland and Økland (2005), Wood and Okamura (2005) , P. repens is common in Britain, Ireland and Europe. Kaminski (1984) described P. repens as most common species in the studied lakes in Poland. Remarks on habitat and ecology in Lithuania. Plumatella repens has been the most commonly encountered species, with floatoblasts occurring in all surveyed sites, include lentic and stagnant habitats. Although colonies were found in only two ponds from listed sites, we have since become aware of colonies occurring in other lakes and ponds not listed here. Pajiedaitė (1933) described P. repens as most common species in Lithuania, which can grow in various freshwater bodies. On the other hand, the exact locations of her collecting sites were not listed in her thesis. In addition, because of early difficulty in identifying this species, distribution reports prior to the mid-1980s are not necessarily reliable (Wood and Okamura 2005) . Remarks. Lacourt (1968) postulated close relationship between Plumatella repens and P. fungosa based on a “short oval statoblasts” and molecular studies confirmed a close relationship between these species (Hirose et al. 2011) . Plumatella repens can be confused with young colonies of P. fungosa (Wood and Okamura 2005). In addition, statoblasts of P. repens are similar to those of its congeners P. nitens Wood, 1996, P. nodulosa Wood, 2001 , P. orbisperma (Kellicott, 1882) , P. recluse Smith, 1992 , and P. rugosa Wood, Wood, Geimer & Massard, 1998 (Massard and Geimer 2008a) . Plumatella geimermassardi Wood & Okamura, 2004 Fig. 3 Material examined. A few floatoblasts from Lampėdžiai Lake in April 2016. P. geimermassardi were recorded in Lithuania for the first time. However, the species is so far represented only by statoblasts. Description. Floatoblasts were identified by the large dorsal fenestra with tubercles and narrow annulus. The annulus at the poles is mostly as large as laterally and is covered by weakly visible tubercles (Fig. 3). Length and width of floatoblast were 311–325 (317±4) μm and 221–273 (244±15) μm (n=3) respectively. L/W ratio 1.3; DfL 199–205 (202±3) μm; DfW 174–201 (187±13) μm (n=3); VfL 200–254 (227±26) μm and VfW 185–198 (192±6) μm (n=3). Distribution in Europe. Plumatella geimermassardi is known from England, Ireland, Belgium, southern Norway, northern Germany, Italy and Finland (Wood and Okamura 2005) . Remarks. Floatoblasts of P. geimermassardi are among the smallest floatoblasts among all European plumatellids with an average length of around 320 μm (Wood and Okamura 2004) . The uniformly narrow annulus offers an easy identifying feature characteristic for broad floatoblasts in this species. The relatively large area of dorsal and ventral fenestrae is matched only by those of P. nitens or Stephanella hina on other continents (Wood 1996; Toriumi 1955) . Plumatella fungosa (Pallas, 1768) Fig. 4 Material examined. A floatoblasts collected from Linksmakalnis pond (June 2015) and Maišia stream (April 2015). Colony from Aristava pond (locates in Kėdainiai district 55°17'07.1"N, 24°04'28.6"E and it is not included in general list of studied sites during this survey) was taken in June 2017 (Fig. 4). Description. The colony dark, spindle shaped, and large (15–17 cm), formed on stems of reeds (Phragmites). Examined floatoblasts exhibited characteristic tubercles on the floatoblast annulus (Fig. 4) and a ridge-like suture between the dorsal and ventral valves. Dorsal floatoblast tubercles were larger on the fenestra than on the annulus. The length of floatoblasts was 324–368 (339±5) μm; width 220–290 (254±8) μm (n=8), L/W ratio 1.3; DfL 130–160 (147±4) μm (n=6); DfW 125–161 (144±5) μm (n=6); VfL 214–250 (227±4) μm and VfW 205–228 (214±2) μm (n=6). However, dimen sions of P. fungosa floatoblasts provided by Pajiedaitė (1933) , were slightly larger: 470 μm × 290 μm. Pajiedaitė also recorded the variability in sessoblast dimensions from different localities: 790 μm × 470 μm in Nevėžis river (Kaunas district); 480 μm × 370 μm in Lake Aukštadvaris (Trakai district), and 580 μm × 420 μm in Snaigynas Lake (Lazdijai district) (Pajiedaite 1933). During current study sessoblasts were not found. Distribution in Europe. According to Wood and Okamura (2005) P. fungosa is widespread in Europe. It has been recorded from several places in southern Sweden and Finland; it is common in Denmark and has been reported from Iceland (Økland and Økland 2005) and Poland (Kaminski 1984) . Remarks on habitat and ecology in Lithuania. During this survey, floatoblasts of P. fungosa were found in seven water bodies from 18 surveyed, with prevalence in stagnant water, with neutral to slightly alkaline pH 7.01–8.15 (Table 1). Pajiedaitė (1933) described the colonies in Kaunas Lagoon, Nevėžis River (Kaunas district) and Dubysa River (Šiauliai district). She noted that P. fungosa often occurred in polluted water and described colonies, found in old port of Kaunas city, where water was polluted by oil of ships and trash. A similar observation has been made by other authors (Bushnell 1966; Geimer and Massard 1986) . Based on the available data, we consider P. fungosa to be prevalent in Lithuania. Remarks. Large bulky colonies of P. fungosa are easily recognizable freshwater bryozoan species in Europe (Wood and Okamura 2004) . Floatoblasts of P. fungosa are lateralyasymmetrical and distinctfrom the symmetrical floatoblasts of P. repens and P. rugosa. Molecular studies showed a close relationship between P. repens and P. fungosa (Hirose et al. 2011) . Plumatella emarginata (Allman, 1844) Fig. 5 Material examined. A few floatoblasts from Šventupė pond were collected in July 2016. Description. Floatoblasts elongated in shape, with a circular ventral fenestra and small dorsal fenestra, covered by tubercles. Floatoblasts were 357–489 (407±18) μm long and 197–235 (216±6) μm (n=6) wide, L/W ratio 1.9; DfL 97–125 (107±9) μm; DfW 60–82 (68±7) μm (n=3); VfL 101–184 (149±9) μm and VfW 110–162 (133±4) μm (n=6). The approximate size of statoblasts provided by Pajiedaite (1933) was 560 μm long and 260 μm wide. Distribution in Europe. Geimer and Massard (1986) defined the range of this species to include most of Europe. Økland and Økland (2005) considered P. emarginata to be a southern species, with limited distribution in Norway and Sweden. Remarks on habitat and ecology in Lithuania. Pajiedaitė (1933) described morphology of P. emarginata colonies, but her text is not clear about the location of collection sites. However, the statoblasts she found were recorded from Paštys Lake (55°42'36"N, 25°41'48"E), Satarečius pond and Dubysa River (Kaunas district, 55°12'12"N, 23°30'28"E). In our survey only a few statoblasts were found in Šventupės pond (Table 1). Wood and Okamura (2005) noted that P. emarginata is particularly tolerant of rapidlyflowing water. The occurrence of floatoblasts in the Neries River (Kaunas district - not included in this study) is consistent with this observation, although colonies were not found. From our data P. emarginata would be considered uncommon in Lithuania, although this should be verified through further surveys. Remarks. The species is widely distributed throughout the Holarctic (Wood and Okamura 2005) , although some reports may have confused it with similar species, P. mukaii or P. reticulata (Massard and Geimer 2008a) . Plumatella casmiana (Oka, 1908) Fig. 6A, B Material examined. Floatoblasts, leptoblasts, and colony from Linksmakalnis pond collected from submerged branches in 20 July 2016. Description. Colony was about 5–6 cm long. Branches of colony are short, almost entirely attached to the substrate. The terminal parts of branches are semi-transparent and whitish. The floatoblasts were recognized by the distinctly elongated shape of the fenestra on both valves. Both capsuled floatoblasts and the distinctive leptoblasts were found, along with associated colonies (Fig. 6A, B). The surface fenestra of capsulated floatoblasts was almost smooth. Length of floatoblasts 345–432 (397±15) μm; width 188–260 (214±14) μm), L/W ratio 1.8; DfL 112–198 (154±15) μm; DfW 90–135 (113±7) μm; VfL 174–236 (205±12) μm; VfW 150–195 (167±8) (n=5). Leptoblasts (Fig. 6B, right side) have a uniformly narrow annulus and extensive oval fenestrae; which length was at least 1.5 times its width. Distribution in Europe. Plumatella casmiana is currently known throughout most of Europe (Massard and Geimer 1995b) . Remarks on habitat and ecology in Lithuania. This is the first reported occur rence of P. casmiana in Lithuania. Floatoblasts of P. casmiana were recorded in almost half of the investigated water bodies (Table 1). However, colonies were found in Linksmakalnis Pond only. Remarks. Beyond Europe P. casmiana is widely distributed through Asia, North America, Africa, and very likely other continents as well (Wood and Okamura 2005) . A unique feature is the appearance of floatoblasts lacking the inner capsule (Figure 6B, right side). This so-called leptoblast is capable of hatching immediately after release from the colony, enabling populations to grow very rapidly each season. Colonies also produce conventional capsuled floatoblasts (Figure 6B, left side) which retain the obligatory dormancy period. Plumatella fruticosa (Allman, 1844) Fig. 7A, B Material examined. Colony from Rokai pond (Kaunas district) found in June 2016; floatoblasts from pond of Kaunas Botanical garden collected in July and August 2016. Description. The colony measured approximately 3 x 4 cm and had sparse, narrow and upright branches. Free statoblasts are long and narrow, exhibiting a length at least twice the width: 432–496 (459±8) μm long and 187–220 (203±4) μm (n=10) wide; L/W ratio 2.2; DfL 120–320 (197±19) (n=10) μm; DfW 56–100 (75±6) (n=6) μm; VfL 211–313 (266±21) (n=4) μm and VfW 74–128 (108±17) μm (n=3). Sessoblasts were not found during this study. According to Pajiedaitė (1933) the average size of the floatoblasts was 590 μm long and 230 μm wide. Distribution in Europe. Plumatella fruticosa is considered to be widespread, especially in northern portion of Europe (Økland and Økland 2005). It is considered common in Poland (Kaminski 1984) . Remarks on habitat and ecology on Lithuania. Pajiedaitė collected colonies in Dubysa river (Šiauliai district) and Satarečius pond (Utena district) (Pajiedaitė 1933) At first glance we could state that P. fruticosa is common in Lithuania, since during this survey statoblasts were found in most water bodies. However, we found colonies only in Rokai pond with sandy-mud bottom and stones in the littoral (Table 1, Fig. 7). Thus, it is possible, that statoblasts are spread by waterfowl among various ponds and lakes, but these may not be the preferred environment for growing colonies (Økland and Økland 2005). Remarks. The combined statoblast characteristics (large length/width ratio, strong asymmetry of floatoblast and sessoblast, narrow fenestra on dorsal floatoblast valve) distinguish P. fruticosa from all other plumatellid species (Ricciardi and Reiswig 1994) . Molecular results provided by Hartikainen (Hartikainen et al. 2013) imply that P. fruticosa is not a plumatellid and provide evidence for polyphyly in Plumatella. However, the position of P. fruticosa remains unresolved (Hartikainen et al. 2013) . Hyalinella punctata (Hancock, 1850) Fig. 8 Material examined. A few statoblasts from Veršvio stream were found in August 2015 Unfortunately, these were later lost before critical dimensions could be taken. Description. Colonies were not observed, and species was identified according floatoblasts. The statoblasts are larger than any other plumatellid species and show crowded tubercles on the fenestrae of both valves. Pajiedaitė (1933) described floatoblasts by oval shape, with length 440 μm and width 230 μm. These dimensions were slightly smaller than 500 μm and 350 μm suggested by Wood and Okamura (2005) . Length and width of measured statoblasts during current study was 425–459 (444±7) and 280–299 (290±4) μm, respectively (n=5). Distribution in Europe. Hyalinella punctata has been widely reported worldwide, including neighbouring Poland (Kaminski 1984) , but verified specimens are known only from Britain, Ireland, Europe, North America and northern Asia (Wood and Okamura 2005) . Remarks on habitat and ecology in Lithuania. Few small colonies of H. punc tata were described on Nymphaea lutea leaves in small lakes in the Zarasai district (55°44'50"N, 25°50'4"E) and Dubysa river (Šiauliai district; 55°51'29"N, 23°08'31"E) by Pajiedaitė (1933) . During the present survey, floatoblasts of H. punctata were recorded only in the Veršvio stream (Table 1). The available data are not sufficient to estimate the prevalence and frequency of this species in Lithuania. Remarks. Hancock (1850) described colonies of H. punctata as “thick and transparent with less profuse branching than in Plumatella and produce only floatoblasts, while individual zooids are indistinct, usually arranged linearly and lack interzooidal septa”. In fact, features distinguishing Hyalinella from Plumatella are not clear-cut (Hirose and Mawatari 2011) , because the diagnosis of Hyalinella is based on the transparency and thickness of the colony wall (ectocyst), but the condition of the ectocyst depends to some extent on environmental factors (Wood and Okamura 2005, Hirose and Mawatari 2011) . Generic placement of some species between Plumatella and Hyalinella has remained unstable (Hirose and Mawatari 2011) . Family Cristatellidae Allman, 1856 Cristatella mucedo (Cuvier, 1798) Fig. 9 Material examined. Colony from Snaigynas lake (Lazdijai district) collected in July 2016, floatoblasts from Rokai pond found in September 2016. Description. Colonies of C. mucedo are recognized by their elongated shape and colourless, transparent body wall. The length of colonies found varied from 5 to 10 cm (Pajiedaitė 1933; this study). The large statoblasts are easily recognized by circular form with hooked spines radiating from the edges of the fenestrae on both valves (Fig. 9). Diameter of statoblasts was about 1 mm. Distribution in Europe. Cristatella mucedo is a common species in Lithuania, with a Holarctic distribution, occurring in Britain, Ireland, Europe, Asia and North America (Økland and Økland 2000; Wood and Okamura 2005) . Remarks on habitat and ecology in Lithuania. During this survey a few colonies of C. mucedo occurred in South Lithuania (Snaigynas lake), but statoblasts were found in various water bodies of different regions of the country (Table 1). Pajiedaitė (1933) noted that C. mucedo more often occurred in South Lithuania. She found colonies of C. mucedo without statoblasts in June/July and noted that statoblasts inside colonies appeared in first part of August. Numerous colonies with statoblasts were found at the end of September and they died late autumn once the water temperature dropped to 3 °C in November 1932 (Pajiedaitė 1933). Remarks. A more detailed discussion of the ecology and life history of C. mucedo can be found in Okamura (1997) . Class Gymnolaemata Allman, 1856 Order Ctenostemata Busk, 1852 Family Paludicellidae Allman, 1844 Paludicella articulata (Ehrenberg, 1831) Fig. 10 Material examined. Two colonies from the outlet of Snaigynas Lake (Lazdijai district) were found in May 2017. Hibernaculae were not found. Description. The species was recognized by the slender colony branches forking at wide angles and often growing free from the substratum. Colonies were small, about 2–3 cm. Branches of colony were transparent and shiny. Zooids 1.0–1.5 mm in length with 16 tentacles on a circular lophophore were described by Pajiedaitė (1933) . Distribution in Europe. Paludicella articulata is known worldwide (Wood and Okamura 2005) . However, the species has not been found in Poland (Kaminski 1984) . Remarks on habitat and ecology in Lithuania. Paludicella articulata was re corded by Pajiedaite (1933) in only two localities: Paštys Lake (Utena district) (55°42'36"N,  25°41'48"E) and Satarečius pond (Utena district). Since P. articulata tolerates cold temperatures (Økland and Økland 2005) and prefers flowing water (Wood and Okamura 2005) it was surprising finding of this species in stagnant Satarečius pond together with C. mucedo. Coexistence of the two species was also noted by Pajiedaitė (1933) , who explained it by different local conditions in the same pond; colonies of P. articulate were observed only near a small stream flowing into the pond. Otherwise, she noted that C. mucedo was mostly observed in the warmer waters of Central and South Lithuania. Økland and Økland (2005) showed positive co-occurrence of these two species in Norway. During this survey P. articulata was found in the outlet of Snaigynas lake, which is of glacial origin and characterised by low temperature. Remarks. Colonies of P. articulata consist of sometimes creeping but more often elongated, mostly erect, slender zooids. There are normally three adjacent zooids: one distal and two lateral ones (Davenport 1891) The contiguous arrangement of the zooids and the subterminal 4-sided zooecial orifice readily distinguish the species from its closest relative, Pottsiella erecta (Ricciardi and Reiswig 1994) . Discussion Overall nine species of freshwater bryozoans are now known from Lithuania. This contrasts with about 19 species reported from Europe and about 13 species from the Baltic area (Massard-Geimer and Massard-Geimer 2004; Nikulina 2006) . Given the fact that only a relatively few water bodies of Lithuania have been investigated so far it is likely that the final tally of species will be higher. The majority of surveyed pools were stagnant, neutral or slightly alkaline (Table  1) and should have been suitable for bryozoans to grow successfully. However, while intact colonies were found only in few sites, statoblasts were widely distributed. The rarity of colonies in water pools might be explained by fluctuating climatic conditions, especially the alternation of drought and rainfall. The similar process under Lithuanian conditions was described by Pajiedaitė (1933) , who found P.  articulata and P. repens colonies in Paštys Lake in 1931, but completely absent the following year. Pajiedaitė (1933) suggested this disappearance may have been due to rising of water level after rainfall in Paštys Lake. She wrote that bryozoans are sensitive “creatures” and cannot survive such drastic environmental change. She went on to describe a similar situation in Nevėžis River in 1932, where after week of rainfall, nearly all bryozoan colonies had died (Pajiedaitė 1933). Jong-Yun-Choi et al. (2015 ) documented the negative effects of heavy rainfall in Korea on colonies of Pectinatella magnifica (Leidy, 1851) . Another possible reason of finding small number of colonies could be the lack of suitable substratum for the attachment of colonies. Because bryozoans are sessile organisms, they absolutely require a solid, inert substratum on which to grow (Ryland 1970) . For example, we have found statoblasts of five bryozoan species in a pond at the Kaunas botanical garden (Table 1). However, colonies have never been observed there, possibly due to lack of solid substratum to which bryozoan colonies can attach. The presence of statoblasts could be the result of waterfowl, which are known to transport them from one site to another (Wood and Okamura 2005) . With this study, we have now recorded 13 species of freshwater bryozoans recorded in Baltic area: C. mucedo, P. magnifica, Fredericella indica Annandale, 1909 , Fredericella sultana (Blumenbach, 1779) , Lophopus crystallinus (Pallas, 1768) , H. punctata, P. casmiana, P. emarginata, P. fruticosa, P. fungosa, P. geimermassardi, P. repens, Stolella indica Annandale, 1909 , and P. articulata (Massard-Geimer and Massard-Geimer 2004; Nikulina 2006) All bryozoan species documented in Lithuania are common and widely distributed through Europe. The composition of species found through this survey was similar to that recorded 86 years ago, with the exception of finding two additional species for Lithuania. The status of P. repens and P. fungosa as common freshwater bryozoan species, and P. articulata as rare, have not changed for almost a century. Curiously, this survey did not encounter F. sultana, which is otherwise well known in northern Europe, Britain and Ireland (Geimer and Massard 1986; Wood and Okamura 2005) . Also absent was the large gelatinous species, P. magnifica, which is currently expanding its range across Europe and is already reported from areas including Hungary (Szekeres et al. 2013), Germany (Grabow 2005) , Czech Republic (Rodriguez and Vergon 2002; Balounova et al. 2011) , and Poland (Balounová et al. 2011). Additional bryozoan species that might be expected in Lithuania include L. crystallinus, which is recorded in neighbouring countries as Belarus, Kaliningrad and Poland (Nikulina 2006); also, P. rugosa, P. reticulata Wood, 1988 , and F. indica. Since the brackish species, Victorella pavida Saville Kent, 1870 , is known in neighbouring Latvia, it is likely to be found also in Lithuania. In summary, we believe that the list of freshwater bryozoa presented here is not final. Lithuania is an extremely watery region; there are more than 3000 lakes of a wide variety of sizes and many rivers flow across the country. Therefore, it is very likely, that further research will reveal additional species. Acknowledgments We sincerely thank Dr. Masato Hirose, University of Tokyo, and anonymous reviewer for their careful reading of and valuable comments on the manuscript. Allman GJ ( 1844 ) Synopsis of the genera and species of freshwater Zoophytes inhabiting Ireland . Annals and Magazine of Natural History, including Zoology, Botany, and Geology: Series 1 , 13 : 328 - 331 . Allman GJ ( 1856 ) A Monograph of the Fresh-Water Polyzoa, Including all the Known Species, both British and Foreign . The Ray Society , London, 119 pp. Annandale TN ( 1909 ) A new species of Fredericella from Indian lakes . Records of the Indian Museum , 3 1909 : 373 - 374 . Balounova Z , Rajchard J , Svehla J , Smahel L ( 2011 ) The onset of invasion of bryozoan Pectinatella magnifica in South Bohemia (Czech Republic) . Biologia 66 : 1091 - 1096 . Bartošová-Sojková P , Hrabcová M , Pecková H , Patra S , Kodádková A , Jurajda P , Tyml T , Holzer AS ( 2014 ) Hidden diversity and evolutionary trends in malacosporean parasites (Cnidaria: Myxozoa) identified using molecular phylogenetics . International Journal Parasitology 44 : 585 - 577 . 2014 . 04 .005 Blumenbach JF ( 1779 ) Handbuch der Naturgeschichte . Göttingen, 559 pp. Borg F ( 1941 ) Über die Süsswasserbryozoen Schwedens . Festkrift tillagnad Prof. Sven Ekman . Zoologiska bidrag från . Uppsala 20 : 479 - 494 . Busk G ( 1852 ) Catalogue of marine Polyzoa in the collection of the British Museum . Vol. 1 . London, British Museum, 54 pp. Bushnell JH ( 1966 ) Environmental relations of Michigan Ectoprocta, and dynamics of natural populations of Plumatella repens . Ecological Monographs 36 : 95 - 123 . https://doi. org/10.2307/1942151 Bushnell JH , Rao KS ( 1974 ) Dormant or quiescent stages and structures among the Ectoprocta: Physical and chemical factors affecting viability and germination of statoblasts . Transactions of the American Microscopical Society 93 ( 4 ): 524 - 543 . Cuvier G (1798) Tableau Élémentaire de l'Histoire Naturelle des Animaux . Baudouin, Paris, 710 pp. Fauna Europea ( 2013 ) Davenport CB ( 1891 ) Observations on budding in Paludicella and some other Bryozoa . Bulletin Museum Comparative Zoology 22 : 1 - 114 . Ehrenberg C ( 1831 ) Phytozoa polypi . Symbolae physicae, etc. Animalia Evertebrata I: 831. Geimer G , Massard JA ( 1986 ) Les bryozoaires du Grand-Duché de Luxembourg et des régions limitrophes . Travaux scientifiques du Musée d'Histoire Naturelle de Luxembourg 7 : 1 - 188 . Grabow K ( 2005 ) Pectinatella magnifica (Leidy, 1851 ) ( Bryozoa) at the Upper Rhine , Germany. Lauterbornia 19 : 133 - 139 . Grabner DS , El-Matbouli M ( 2008 ) Transmission of Tetracapsuloides bryosalmonae (Myxozoa: Malacosporea) to Fredericella sultana (Bryozoa: Phylactolaemata) by various fish species . Diseases of Aquatic Organisms 79 : 133 . Gruncharova T ( 1968 ) Beträge zur Erforschung der Briozoischen süsswasserfauna in Bulgaria . Bulletin de l' Institut de Zoologie et Musée 26 : 39 - 46 . Hancock A ( 1850 ) On the anatomy of the freshwater Bryozoa, with descriptions of three new species . The Annals and Magazine of Natural History; Zoology, Botany, and Geology 5 ( 2 ): 173 - 204 . Hartikainen H , Waeschenbach A , Wöss E , Okamura B , Wood T ( 2013 ) Divergence and species discrimination in freshwater bryozoans (Bryozoa: Phylactolaemata) . Zoological Journal of the Linnean Society 168 ( 1 ): 61 - 80 . Hirose M , Dick MH , Mawatari SF ( 2011 ) Are plumatellid statoblasts in freshwater bryozoans phylogenetically informative? Zoological Science 28 ( 5 ): 318 - 326 . zsj.28.318 Hirose M , Mawatari SF ( 2011 ) Freshwater Bryozoa of Okinawa, Japan, with descriptions of Rumarcanella gen. nov. (Phylactolaemata: Plumatellidae) and two new species . Zootaxa 2732 : 1 - 19 . Jong-Yun Ch , Gea-Jae J , Dong-Gyun H , Hyunbin J ( 2015 ) Importance of substrate material for sustaining the bryozoan Pectinatella magnifica following summer rainfall in lotic freshwater ecosystems, South Korea . Journal of Ecology and environment 38 (3) : 375 - 381 . 2015 .039 Jullien J ( 1885 ) Monographie des Bryozoaires d'eau douce . Bulletin de la Société Zoologique de France 10, 91 - 207 . Kaminski M ( 1984 ) Food composition of three bryozoan species (Bryozoa, Phylactolaemata) in a mesotrophic lake . Polskie Archiwum Hydrobiologii 31 : 45 - 53 . Kellicott DS ( 1882 ) Polyzoa. Observations on species detected near Buffalo . Proceedings of the American Society of Microscopists 4 : 217 - 229 . Kraepelin K ( 1887 ) Die deutschen Süsswasser-Bryozoen . Eine Monographie. Abhandlungen Naturwissenschaftlicher. Verein Hamburg 10 : 168 . Lacourt AW ( 1949 ) Bryozoa of the Netherlands . Archives Neéderlandaises de Zoologie 8 ( 3 ): 289 - 321 . Lacourt AW ( 1968 ) A monograph of the fresh-water Bryozoa-Phylactolaemata . Zoologische Verhandelingen 93 : 1 - 159 . Leidy J ( 1851 ) On Cristatella magnifica n. sp . Proceedings of the Academy of Natural Sciences of Philadelphia 5 : 265 - 266 . Linnaeus C (1758) Systema Naturae , Vol. 1 , 10th Edn , 873 pp. Loppens K ( 1906 ) Contribution à l'étude des Bryozoaires de la Belgique . Bulletin de la Société Royale de Zoologie et Malacologie du Belgie 41 : 130 - 136 . Massard JA , Geimer G ( 1995b) On the distribution of Plumatella casmiana in the European and Mediterranean parts of the Palaearctic region (Bryozoa, Phylactolaemata) . Bulletin de la Société des Naturalistes Luxembourgeois , 96 : 157 - 165 . Massard-Geimer J , Massard-Geimer G ( 2004 ) Fauna Europaea: Bryozoa (Entoprocta) . Fauna Europaea version 1 .1 Massard JA , Geimer G ( 2005 ) Die Süsswasser Bryozoen in der Fauna Europea 2004: Karten und Kommentare . In: Wöss ER (Ed.) Moostiere (Bryozoa), Biologiezentrum/Oberösterreichische Landesmuseen, Linz, 167 - 174 . Massard JA , Geimer G ( 2008a ) Global diversity of bryozoans (Bryozoa or Ectoprocta) in freshwater . Hydrobiologia 595 : 93 - 99 . Massard JA , Geimer G ( 2008b) Occurrence of Plumatella emarginata Allman 1844 and P. casmiana Oka, 1908 (Bryozoa Phylactolaemata) in Lake Pamvotis (Ioannina, Greece) . Société des Naturalistes Luxembourgeois 109 : 133 - 138 . Mundy SP ( 1980 ) A Key to the British and European Freshwater Bryozoans . Freshwater Biological Association, London. Scientific Publication No. 41 . Nikulina EA ( 2006 ) Bryozoans of the Baltic Sea . Meyniana 58 : 75 - 95 . Oka A ( 1907 ) Zür Kenntnis der Süesswasser-Bryozoenfauna von Japan . Annotationes Zoologicae Japonenses 6 ( 2 ): 117 - 123 . Okamura B ( 1997 ) The ecology of subdivided populations of a clonal freshwater bryozoan in southern England . Archiv fur Hydrobiologie 141 : 13 - 34 . Økland KA , Økland J ( 2000 ) Freshwater bryozoans (Bryozoa) of Norway: Distribution and ecology of Cristatella mucedo and Paludicella articulata . Hydrobiologia 421 : 1 - 24 . https:// Økland KA , Økland J , Geimer G , Massard JA ( 2003 ) Freshwater bryozoans (Bryozoa) of Norway IV: distribution and ecology of four species of Plumatella with notes on Hyalinella punctata . Hydrobiologia 501 : 179 - 198 . Økland KA , Økland J ( 2005 ) Freshwater bryozoans (Bryozoa) of Norway, V: review and comparative discussion of the distribution and ecology of the 10 species recorded . Hydrobiologia 534 : 31 - 55 . Pajiedaitė B ( 1933 ) Contribution to the exploration of freshwaters bryozoans in Lithuania . Kaunas, Lithuania: University of Vytautas Magnus. Unpublished master thesis . [In Lithuanian] Pallas PS (1768) Descriptio Tubulariae fungosaa prope Volodemirum mense Julio 1768 observatae Novi commentarii Academiae Scientiarum Imperialis Petropolitanae 12 : 565 - 572 . Ricciardi A , Reiswig HM ( 1994 ) Taxonomy, distribution and ecology of the freshwater bryozoans (Ectoprocta) of eastern Canada . Canadian Journal of Zoology 72 : 339 - 359 . https:// 048 Rodriguez S , Vergon JP ( 2002 ) Pectinatella magnifica ( Leidy 1851 ) (Phylactolaemata), a species of bryozoan introduced in the north of Franche-comte . Bulletin français de la pêche et de la pisciculture 365-366: 281 - 296 . Ryland JS ( 1970 ) Bryozoans . Hutchinson University Library, London, 175 pp. Satkauskiene I , Asmantas S , Mildaziene V , Wood TS ( 2018 ) Freshwater bryozoans of Lithuania . Annals of Bryozoology 6 : 75 - 83 . Saville-Kent W ( 1870 ) On a new polyzoon, Victorella pavida, from the Victoria Docks . Quarterly Journal of Microscopical Science 10 ( 2 ): 35 - 39 . Smith D ( 1992 ) A new freshwater moss animal in the genus Plumatella (Ectoprocta: Phylactolaemata: Plumatellidae) from New England (USA) . Canadian Journal of Zoology 70 : 2192 - 2201 . 296 Smyth T ( 1994 ) The distribution of freshwater Bryozoa in Ireland . Bulletin of the Irish Biogeographic Society 17 : 9 - 21 . Szekeres J , Akác A , Csányi B ( 2013 ) First record of Pectinatella magnifica (Leidy 1851) in Hungary . Water Research and Management 3 ( 4 ): 35 - 40 . Toriumi M ( 1955 ) Taxonomical study on freshwater Bryozoa. XI. Stephanella hina Oka . Science Reports of Tôhoku University, Ser. 4 , 21 ( 2 ): 131 - 136 . Trauberg O ( 1940 ) Beitrag zur Kenntnis einiger in Letlland vorhommender Susswasser-BryozoaArten . Folia Zoologica et Hydrobiologica 10 ( 2 ): 479 - 484 . Viganó ( 1965 ) Nuovi dati sui Briozoi dell acque interne italiane . Bollettinodi Zoologia 32 ( 2 ): 913 - 928 . Wood TS ( 1988 ) Plumatella reticulata sp. nov. in Ohio (Bryozoa: Phylactolaemata) . Ohio Journal of Science 88 ( 3 ): 101 - 104 . Wood TS ( 1996 ) Plumatella nitens, a new species of freshwater bryozoan from North America (Ectoprocta: Phylactolaemata), previously misidentified . Hydrobiologia 328 : 147 - 153 . Wood TS , Wood LJ , Geimer G , Massard J ( 1998 ) Freshwater bryozoans of New Zealand: a preliminary survey . New Zealand Journal of Marine and Freshwater Research 32 : 639 - 648 . 1998 .9516850 Wood TS ( 2001 ) Three new species of plumatellid bryozoans (Ectoprocta: Phylactolaemata) defined by statoblast nodules . Journal of the North American Benthological Society 20 ( 1 ): 133 - 143 . Wood TS , Okamura B ( 2004 ) Plumatella geimermassardi, a newly recognized freshwater bryozoan from Britain, Ireland, and continental Europe (Bryozoa: Phylactolaemata) . Hydrobiologia 518 ( 1-3 ): 1 - 7 . 0000025051 .65713.85 Wood TS , Okamura B ( 2005 ) A new key to the Freshwater Bryozoans of Britain, Ireland and Continental Europe, with notes on their Ecology . Fresh Water Biological Association , Ambleside, UK , Scientific Publication 63 : 1 - 113 . Wood TS , Anurakpogsatorn P , Chaichana R , Manujchariyawong J , Satapanajaru T ( 2006 ) Predation on freshwater bryozoans by the golden apple snail , Pomacea canaliculata Lamarck , 1822 (Ampullariidae). The Natural History Journal of Chulalongkorn University 6 ( 1 ): 31 - 36 . 0283 - 0286 .pdf

This is a preview of a remote PDF:

Ingrida Šatkauskienė, Timothy Wood, Jurgita Rutkauskaitė-Sucilienė, Vida Mildažienė, Simona Tučkutė. Freshwater bryozoans of Lithuania (Bryozoa), ZooKeys, 2018, 53-75, DOI: doi:10.3897/zookeys.774.21769