Studies of hereditary-obese mice (obob) after implantation of pancreatic islets in Millipore filter capsules

Diabetologia, Jun 1970

Summary Obese mice were implanted with Millipore diffusion chambers containing islets isolated from pancreas of normal littermates. Controls in this study were either obese implanted with encapsulated obese islets and empty chambers or non-obese implanted with encapsulated obese islets, non-obese islets and empty chambers. — Regular checks were made on all mice for weight gains and glucose levels. In addition, samples of blood were pooled from each group for immunoreactive insulin determinations. All mice, except one-half the experimental group, were sacrificed at 45 days andpost-mortem examinations performed. The capsules containing non-obese islets were removed from the remaining obese mice and weight gains followed for an additional 45 days. These mice were then sacrificed and determinations made for glucose and insulin levels. — The results showed that weight gain was stabilized, glucose and insulin levels in obese mice were reduced within 14 days after implanting Millipore diffusion chambers containing non-obese pancreatic islets. Weight gain resumed immediately and elevated glucose and insulin levels were found 45 days after removing Millipore diffusion chamber. — These results led to the following conclusions: 1. The spontaneous obesity which develops in the hereditary obese mouse results from a missing or defective pancreatic islet factor or factors. — 2. This factor originates from the islets of Langerhans and is capable of passing through 0.45 μ pores of the Millipore membrane. — 3. This factor's presence is necessary for normal glucose and lipid metabolism and insulin sensitivity.

A PDF file should load here. If you do not see its contents the file may be temporarily unavailable at the journal website or you do not have a PDF plug-in installed and enabled in your browser.

Alternatively, you can download the file locally and open with any standalone PDF reader:

https://link.springer.com/content/pdf/10.1007%2FBF01212243.pdf

Studies of hereditary-obese mice (obob) after implantation of pancreatic islets in Millipore filter capsules

Studies of Hereditary-Obese Mice (obob) after Implantation of Pancreatic Islets in Millipore Filter Capsules R . L . STI~ 0 0 D e p a r t m e n t of Biology, The American University , Washington D. C., U S A , USA Summary. Obese mice were implanted with Millipore diffusion chambers containing islets isolated from pancreas of normal littermates. Controls in this study were either obese implanted with encapsulated obese islets and e m p t y chambers or non-obese implanted with encapsulated obese islets, non-obese islets and e m p t y chambers. -- l~egular cheeks were made on all mice for weight gains and glucose levels. I n addition, samples of blood were pooled from each group for immunoreactive insulin determinations. All mice, except one-half the experimental group, were sacrificed at 45 days and post-mortem exami~ nations performed. The capsules containing non-obese islets were removed from the remaining obese mice and weight gains followed for an additionaI 45 days. These mice were then sacrificed and determinations made for glucose and insulin levels. -- The results showed t h a t weight gain was stabilized, glucose and insulin levels in obese mice were reduced within 14 days after implanting Millipore diffusion chambers containing non-obese pancreatic islets. Weight gain resumed immediately and elevated glucose and insulin !evels wore found 45 days after removing Millipore diffusion chamber. -- These resuits led to the following conclusions: 1. The spontaneous obesity which develops in the hereditary obese mouse results from a missing or defective pancreatic isIet factor or factors. -- 2. This factor originates from the islets of Langerhans and is capable of passing through 0.45 ~ pores of the Millipore membrane. -- 3. This factor's presence is necessary for normal glucose and Iipid metabolism and insulin sensitivity. Etude de la souris obob aprgs implantation d'~lots pancrdatiques dans des capsules Millipore Rgsumd. Des ehambres ~ diffusion Millipore contenant des dots isolds de paner4as d ' a n i m a u x n o r m a u x out dt6 implant4es ~ des souris obbses. Dens cette 6rude, los anim a u x t4moins 4taient soit des animaux ob@ses implantds avec des ilots eneapsulds d ' a n i m a u x oh@sea ou avec des chambres vides, soit des animaux non-ob@ses implant4s avee des ilots oneapsul4s d ' a n i m a u x ob~ses, non ob@ses et avee des chambres rides. Des contr61es r4guliers ont 4t4 effectu4s chez tous les animaux quant au gain de poids et aux glyc4mies. De plus, des mesures de 'insuline immunor4active ont 4t4 effectugs sur des 4chantillons de sang provenant de plusiem-s animamx de cheque groupe. -Toutes les souris, ~ I'exception de la moiti4 du groupe exp4rimental, out 4t4 sacrifi4es au 45e jour de l'exp4rienee et des examens post-mortem ont dr4 effectuds. Los capsules contenant des ilots d ' a n i m a u x non-obbses out 4t4 retir4 des souris ob~ses restantes et le gain de poids a 4t4 suivi pour une entre pdriode de 45 jours. Apr@s sacrifice de cos souris, des mesures de glyc4mies et des t a u x d'insuline out dt4 effectuges. -- Los r4sultats de eette exp4rience out montr4 que le gain de poids s'4tait stabilis4, que los glycgmies et los t a u x d'insuline plasmatique des souris ob@ses s'dtaient r4duits au tours des 14 jours suivant l'implantation des ehambres Millipores contenant des ilots paner6atiques des animaux non-ob~ses. Le gain de poids reprenait imm6diatement et lea glyegmies et les t a u x d'insuline plasmatiques 41evds ont 4t4 trouv4s 45 jours aprbs I'ablation des chambres de diffusion Millipore. -- Les r4sultats de cette dtude nous e m i n e n t aux conclusions suivantes: 1. L'ob4sit6 spontange qui se dgveloppe chez la souris ayant une obgsit4 h4r6ditaire r4sulte d'une terence relative ou absolue d ' u n ou de plusieurs facteurs insulaires pancrgatiques. 2. Co facteur est originaire des ilots de Langerhans et il est capable de traverser los pores de 0.45 ~ des filtres Millipore. 3. La prgsence de ce faeteur est ngcessaire pour maintenir aussi bien une glyc4mie normale qu'uno sensibilit4 ~ l'insuline et un mdtabolisme lipidique normaux. Untersuchung der obob M a u s nach Implantation yon Langerhans'schen Inseln in Milliporekammern Zusammenfassung. Isolierte Langerhans'sche Inseln normalgewichtiger Geschwister wurden in Millipore-Kapseln in die PeritonealhShle yon obob Mgusen implantiert. Als Kontrollen dienten obob M/iuse, denen yon obob erhaltene Inseln odor leere Milliporekammern implantiert wurden und normale Tiere, die entweder Inseln yon obob, yon Kontrolltieren odor leere K a m m e r n trugen. I n regelm/il?igen Abstiinden wurden K6rpergewicht und Blutzuckerkonzentration kontrolliert. Augerdem wurden bei dieser Gelegenheit Blutproben allot Tiere zur radioimmunologisehen Bestimmung der Insulinkonzentration gepoolt. Nach 45 Tagen wurden, mit Ausnahme der H/ilfte der mit Normalinseln implantierten obob M/~use, s/imtliche Tiere getStet. Bei den iiberlebenden obob M/iusen wurden die von Nor (...truncated)


This is a preview of a remote PDF: https://link.springer.com/content/pdf/10.1007%2FBF01212243.pdf

R. L. Strautz. Studies of hereditary-obese mice (obob) after implantation of pancreatic islets in Millipore filter capsules, Diabetologia, 1970, pp. 306-312, Volume 6, Issue 3, DOI: 10.1007/BF01212243