A PDF file should load here. If you do not see its contents
the file may be temporarily unavailable at the journal website
or you do not have a PDF plug-in installed and enabled in your browser.
Alternatively, you can download the file locally and open with any standalone PDF reader:
https://link.springer.com/content/pdf/10.1007%2Fs00439-011-1069-7.pdf
Young Min Kwon, Steven C. Ricke: High throughput next generation sequencing, methods and applications. Methods in molecular biology
Humana Press
0
Michael A. Quail
0
0
M. A. Quail (&) The Wellcome Trust Sanger Institute
, Room H134,
Wellcome Trust Genome Campus
, Hinxton, Cambs CB10 1SA,
UK
-
I like books! And despite my best attempts it took me a
long time to like this one. Like Robert Louis Stevensons
Jeckyll and Hyde, the book has dual personalities. Early on
there are three chapters describing protocols for the
Helioscope. These chapters contain considerable repetition
and are a little pointless as there are only one or two
Institutes in the world that operate this platform. Likewise
there are several similar chapters on microbial diversity
and 454 sequencing that again are very repetitious. I wish I
had a sum of money for every time I had read that only a
very small fraction of the planets microbial community
can be cultured. A couple of chapters just describe the use
of particular kits and are little more than a repeat of the
manufacturers protocols.
However, there are definitely some highlights that will
make this book of use to both NGS Newbies and
experienced practitioners. For example
Chapter 2 describes a method for WGA of uncultured
bacterium and seems well balanced mentioning the
known problems with this approach.
Chapter 5 provides protocols for preparing next gen
sequencing libraries of bacterial small RNAs.
Chapter 9 describes how to do amplicon sequencing on
the 454 platform.
Chapter 15 describes how to sequence from and
determine the position of transposon insertions, a
valuable technique for identifying essential genes for
particular growth conditions.
Chapter 16 gives details of two approaches for
determination of DNA methylation patterns.
Chapter 17 reviews the Nextera NGS library preparation
approach.
Chapter 18 describes how to make Illumina sequencing
libraries without PCR and so avoid amplification bias.
Chapter 19 describes a method for target selection using
hairpin selector probes.
Chapter 20 provides information on how to multiplex up
to 96 libraries in a single Illumina lane.
Overall then it is worthwhile but it is not a Sambrook
and Russell (or Maniatis of old) that you turn to time and
time again. Nor is it what it says on the cover. There are
huge areas of NGS methods and applications missing.
Whilst there are three chapters dedicated to Helicos
sequencing there are no applications for SOLiD. Nor are
there any general RNA seq or ChIP seq chapters. Also
missing are protocols for preparation of mate pair libraries.
However, whilst it is often said that books are not useful
as they are out of date as soon as they are published this is
not the case here and many readers will find this a very
useful book.
(...truncated)