Solution to peptide sequencing challenge

Analytical and Bioanalytical Chemistry, Jan 2013

Juris Meija

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Solution to peptide sequencing challenge

Juris Meija 0 0 The winner of the peptide sequencing challenge (published in volume 404, issue 4) is Sami T. Tuomivaara, Complex Carbohydrate Research Center, University of Georgia , Athens , USA The award entitles the winner to select a Springer book of his choice up to a value of 75. Our congratulations! 1 ) National Research Council Canada , Ottawa , ON K1A 0R6, Canada Fig. 1 Kalata, 2002, painted steel, length 3 ft (90 cm), by Julian Voss-Andreae [4]. The pictured sculpture is based on the structure of the protein kalata B1 Since this disulfide connectivity cannot be drawn on a plane without any disulfide bonds crossing, kalata B1 forms a nonplanar graph commonly known as the cyclic cystine knot [3]. - The cyclic peptide described is kalata B1 [1, 2] and its structure is shown in Fig. 1. The partial reduction of the cyclic peptide [2, 3] allows one to establish the disulfide connectivity. The first peptide is amino-ethylated at only {2}Cys and {5}Cys, whereas the second peptide is amino-ethylated at only {1}Cys and {4}Cys. The original peptide therefore contains disulfide bridges at {2} Cys and {5}Cys and at {1}Cys and {4}Cys, and consequently also at {3}Cys and {7}Cys.


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Juris Meija. Solution to peptide sequencing challenge, Analytical and Bioanalytical Chemistry, 2013, 17, DOI: 10.1007/s00216-012-6505-6