Nephrology Dialysis Transplantation, May 2012
Introduction and Aims: To explore the effect and mechanism of fluvastatin on the expression of fibronectin(FN) in human peritoneal mesothelial cells(HPMC) induced by high-glucose peritoneal dialysate. Methods: Cultured HPMC were randomly divided into si × groups of control(a 1:1 mixture of DMEM and total culture fluid), high-glucose peritoneal dialysate(a 1:1 mixture of 4.25% peritoneal dialysis solusions and total culture fluid, HGPDS), HGPDS plus fluvastatin 10-8mol/L, 10- 7mol/L, 10-6mol/L, HGPDS plus GSK650394 10-5mol/L(the competitive inhibitor of SGK1), fluvastatin 10-6mol/L and GSK650394 10-5mol/L . The cellular viability were detected by MTT colorimetry, the mRNA expressions of serum and glucocorticoid -inducible kinase 1(SGK1) and FN were detected by RT-PCR. Results: The results of MTT revealed that the groups of DMSO and different concentrations of fluvastatin had no significant effect on viability of HPMC(P > 0.05), while lower HPMC viability was found in the groups of HGPDS, HGPDS plus different concentrations of fluvastatin and HGPDS plus GSK650394 (P < 0.05). Compared with the HGPDS group, the viability of HPMC was improved significantly in HGPDS + fluvastatin 10-6mol/L group and HGPDS + GSK650394 group (P < 0.05)at the time of 24h.Compared with the normal control group, the mRNA expression of SGK1 and FN increased significantly in HPMC treated with HGPDS, which peaked at 1h and 6h respectively. GSK650394 significantly decreased the high mRNA expression of SGK1 and FN induced by HGPDS (P < 0.05), and the fluvastatin significantly inhibited the activations of SGK1 and FN mRNA induced by HGPDS in a dose- dependent manner(P < 0.05). Conclusions: High-glucose peritoneal dialysate can increase FN expression in human peritoneal mesothelial cells, which can be decreased by fluvastatin. The role of fluvastatin may partially achieved through the signal pathway of SGK1.
This is a preview of a remote PDF: https://ndt.oxfordjournals.org/content/27/suppl_2/ii457.full.pdf
Jia Liu, Jia Liu, Yanchun Liu, Yaguang Xu, Xiufen Zhao, Jun Qian, Bin Sun, Changying Xing, Reo Kanda, Chieko Hamada, Takanori Nakano, Keiichi Wakabayashi, Hiroaki Io, Satoshi Horikoshi, Yasuhiko Tomino, Nana Ishimatsu, Tetsu Miyamoto, Hiroyuki Morimoto, Junichi Nakamata, Ryoko Baba, Kaori Kanegae, Ryota Serino, Narutoshi Kabashima, Yutaka Otsuji, Yoshiaki Doi, Masahito Tamura, Junichi Nakamata, Hiroyuki Morimoto, Ryoko Baba, Nana Ishimatsu, Tetsu Miyamoto, Kaori Kanegae, Ryota Serino, Narutoshi Kabashima, Yutaka Otsuji, Yoshiaki Doi, Masahito Tamura, Takuo Kusumoto, Kei Fukami, Sho-Ichi Yamagishi, Seiji Ueda, Yusuke Kaida, Takuma Hazama, Yosuke Nakayama, Ryotaro Ando, Nana Obara, Seiya Okuda, Masahito Tamura, Mika Matsumoto, Tetsu Miyamoto, Kaori Kanegae, Yumi Furuno, Ryota Serino, Narutoshi Kabashima, Yutaka Otsuji, Hsu Bang-Gee, Luisa Mazzotta, Alberto Rosati, Antonio Carlini, Viviana Teixeira Henriques, Edson Zangiacomi Martinez, José Carolino Divino-Filho, Roberto Pecoits-Filho, José Abrão Cardeal Da Costa, Viviana Teixeira Henriques, Viviana Teixeira Henriques, Thiane Gama Axelsson, Bengt Lindholm, Juan Jesus Carrero, Olof Heimburger, Peter Stenvinkel, Abdul Rashid Qureshi, Masanobu Akazawa, Tomomi Uno, Eiichiro Kanda, Yoshitaka Maeda, Maria Aktsiali, Maria Aktsiali, Sofia Antonopoulou, Konstantina Tsiolaki, Neratzoula Bakirtzi, Alexandra Patrinou, Maria Georgopoulou, Paraskevi Liaveri, Nikolaos Afentakis, George Tsirpanlis, Takeshi Hasegawa, Hiroki Nishiwaki, Makoto Hirose, Daisuke Komukai, Hironori Tayama, Fumihiko Koiwa, Ashio Yoshimura, Sing Leung Lui, Sl Lui, Susan Yung, Colin Tang, Flora Ng, Wai Kei Lo, Tak Mao Chan, Hyang Mo Koo, Fa Mee Doh, Dong Eun Yoo, Hyung Jung Oh, Tae-Hyun Yoo, Kyu Hun Choi, Shin-Wook Kang, Dae Suk Han, Seung Hyeok Han, Natalia Fernandes, Natália Fernandes, Marcus Gomes Bastos, Márcia Regina Gianotti Franco, Alfredo Chaoubah, Maria Da Glória Lima, Roberto Pecoits-Filho, José Carolino Divino-Filho, Abdul Rashid Qureshi, Seokhui Kang, Junyoung Do, Kyuhyang Cho, Jongwon Park, Kyungwoo Yoon, , Jin-Bor Chen, Ben-Chung Cheng, Te-Chuan Chen, Yu-Jen Su, Chien-Hsing Wu, , Yongki Park, Jimin Jeon, Maria Tsikeloudi, Panagiotis Pateinakis, Katerina Patsatsi, Eleni Manou, Dimitrios Sotiriadis, Dimitrios Tsakiris, Laetitia Teixeira, Anabela Rodrigues, M.J. Carvalho, António Cabrita, Denisa Mendonça, Seokhui Kang, Junyoung Do, Jongwon Park, Kyuhyang Cho, Kyungwoo Yoon, Maurizio Bruschi, Giovanni Candiano, Laura Santucci, Salvatore Luzio, Rossella Cannavò, Gian Marco Ghiggeri, Enrico Verrina, Yogesh Varadarajan, Balasubramaniyam Raju, Kyu-Hyang Cho, Junyoung Do, Seokhui Kang, Jong-Won Park, Kyung-Woo Yoon, Tae-Woo Kim, Martin Kimmel, Niko Braun, Jörg Latus, Mark Dominik Alscher, Dirk Struijk, Sadie Van Esch, Raymond T. Krediet, Natalia Fernandes, Tessa Van den Beukel, Tiny Hoekstra, Luciana Tirapani, Kleyton De Andrade Bastos, Roberto Pecoits-Filho, Abdul Rashid Qureshi, Marcus Bastos, Friedo Dekker, José Carolino Divino-Filho, Tamura Yasuhisa, Hidetoshi Kanai, Kenji Harada, Yasuhiro Kawai, Hitoshi Sugiyama, Yasuhiko Ito, Kazuhiko Tsuruya, Hisako Yoshida, Hiroki Maruyama, Shin Goto, Masaaki Nakayama, Hidetomo Nakamoto, Hiroshi Morinaga, Seiichi Matsuo, Hirofumi Makino, María Cristina DI Gioia, Paloma Gallar, Nuria Laso, Isabel Rodriguez, Gabriela Cobo, Aniana Oliet, Julie Hynostroza, Juan Carlos Herrero, Carmen Mon, Milagros Ortiz, Ana Vigil, Tadashi Tomo, Jose Portolés, Simona Uta, Simona Uta, Ana M Tato, Paula Lopez-Sanchez, Maite Rivera, Rosely Rodriguez-Pena, Gloria Del Peso, Mayra Ortega, Carmen Felipe, Eirini Tsampikaki, Georgios Aperis, Aristeides Kaikis, Christos Paliouras, Nikolaos Karvouniaris, Maria Maragaki, Polichronis Alivanis, Birgit Kortus-Götze, Thomas Höferhüsch, Joachim Hoyer, Francesca Martino, Manish Kaushik, Maria Pia Rodighiero, Carlo Creapldi, Claudio Ronco, Antonio Lacquaniti, Antonio Lacquaniti, Valentina Donato, Maria Rosaria Fazio, Silvia Lucisano, Valeria Cernaro, Rosaria Lupica, Michele Buemi, Carmela Aloisi, Tomomi Uno, Masanobu Akazawa, Eiichiro Kanda, Yoshitaka Maeda, Nuket Bavbek Ruzgaresen, Sema Secilmis, Hakki Yilmaz, Ali Akcay, Murat Duranay, Nilgül Akalin, Nilgül Akalin, Mehmet Riza Altiparmak, Sinan Trabulus, Ayse Serap Yalin, Rezzan Ataman, Kamil Serdengeçti, Karoly Schneider, Balintné Bator, Braun Niko, Niko Braun, Fritz Peter, Christoph Ulmer, Latus Joerg, Kimmel Martin, Biegger Dagmar, Ott German, Reimold Fabian, Dippon Juergen, Segerer Stephan, Alscher Dominik, Joerg Latus, Joerg Latus, Christoph Ulmer, Peter Fritz, Bianka Rettenmaier, Sandra Hirschburger, Stephan Segerer, Dagmar Biegger, Thomas Lang, German Ott, Martin Kimmel, Mark Dominik Alscher, Niko Braun, Meelad Habib, Mario Korte, Martijn Hagen, Frank Dor, Michiel Betjes, Meelad Habib, Martijn Hagen, Mario Korte, Robert Zietse, Frank Dor, Michiel Betjes, Joerg Latus, Joerg Latus, Christoph Ulmer, Peter Fritz, Bianka Rettenmaier, Dagmar Biegger, Thomas Lang, German Ott, Christoph Scharpf. Peritoneal dialysis, Nephrology Dialysis Transplantation, 2012, pp. ii457-ii482, 27/suppl 2, DOI: 10.1093/ndt/gfs243