Peritoneal dialysis

Nephrology Dialysis Transplantation, May 2012

Introduction and Aims: To explore the effect and mechanism of fluvastatin on the expression of fibronectin(FN) in human peritoneal mesothelial cells(HPMC) induced by high-glucose peritoneal dialysate. Methods: Cultured HPMC were randomly divided into si × groups of control(a 1:1 mixture of DMEM and total culture fluid), high-glucose peritoneal dialysate(a 1:1 mixture of 4.25% peritoneal dialysis solusions and total culture fluid, HGPDS), HGPDS plus fluvastatin 10-8mol/L, 10- 7mol/L, 10-6mol/L, HGPDS plus GSK650394 10-5mol/L(the competitive inhibitor of SGK1), fluvastatin 10-6mol/L and GSK650394 10-5mol/L . The cellular viability were detected by MTT colorimetry, the mRNA expressions of serum and glucocorticoid -inducible kinase 1(SGK1) and FN were detected by RT-PCR. Results: The results of MTT revealed that the groups of DMSO and different concentrations of fluvastatin had no significant effect on viability of HPMC(P > 0.05), while lower HPMC viability was found in the groups of HGPDS, HGPDS plus different concentrations of fluvastatin and HGPDS plus GSK650394 (P < 0.05). Compared with the HGPDS group, the viability of HPMC was improved significantly in HGPDS + fluvastatin 10-6mol/L group and HGPDS + GSK650394 group (P < 0.05)at the time of 24h.Compared with the normal control group, the mRNA expression of SGK1 and FN increased significantly in HPMC treated with HGPDS, which peaked at 1h and 6h respectively. GSK650394 significantly decreased the high mRNA expression of SGK1 and FN induced by HGPDS (P < 0.05), and the fluvastatin significantly inhibited the activations of SGK1 and FN mRNA induced by HGPDS in a dose- dependent manner(P < 0.05). Conclusions: High-glucose peritoneal dialysate can increase FN expression in human peritoneal mesothelial cells, which can be decreased by fluvastatin. The role of fluvastatin may partially achieved through the signal pathway of SGK1.

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Peritoneal dialysis

Junichi Nakamata 2 9 10 13 21 24 25 29 36 38 40 43 45 49 54 57 62 65 Hiroyuki Morimoto 1 8 12 19 13 23 25 28 35 37 39 42 44 48 51 53 56 61 66 Ryoko Baba 1 8 12 19 13 23 25 28 35 37 39 42 44 48 51 53 56 61 66 Nana Ishimatsu 2 9 10 13 21 24 25 29 36 38 40 43 45 49 54 57 62 65 Tetsu Miyamoto 2 9 10 13 21 24 25 29 36 38 40 43 45 49 54 57 62 65 Kaori Kanegae 0 7 11 18 20 22 25 27 34 35 41 52 55 60 65 Ryota Serino 2 9 10 13 21 24 25 29 36 38 40 43 45 49 54 57 62 65 Narutoshi Kabashima 0 7 11 18 20 22 25 27 34 35 41 52 55 60 65 Yutaka Otsuji 2 9 10 13 21 24 25 29 36 38 40 43 45 49 54 57 62 65 Yoshiaki Doi 1 8 12 19 13 23 25 28 35 37 39 42 44 48 51 53 56 61 66 Masahito Tamura 0 7 11 18 20 22 25 27 34 35 41 52 55 60 65 0 The Kidney Center, University of Occupational and Environmental Health , Kitakyushu, Japan 1 The Department of Anatomy, University of Occupational and Environmental Health , Kitakyushu, Japan 2 The 2nd Department of Internal Medicine, University of Occupational and Environmental Health , Kitakyushu, Japan 3 Kidney Center, University of Occupational And Environmental Health ,Kitakyusyu, Japan 4 The Second Department of Internal Medicine,University of Occupational And Environmental Health ,Kitakyusyu, Japan 5 Kidney Center, University of Occupational And Environmental Health , Kitakyusyu, Japan 6 The Second Department of Internal Medicine,University of Occupational And Environmental Health ,Kitakyu, Japan 7 Division of Nephrology, Department of Medicine, Kurume University, School of Medicine 8 Department of Pathophysiology and Therapeutics of Diabetic Vascular Complications, Kurume University School of Medicine 9 Division of Nephrology, Department of Medicine, Kurume University School of Medicine 10 Department of Nephrology, General Hospital of Athens , g. Gennimatas, Athens, Greece 11 Clinica Gamen - Rio de Janeiro 12 Interdisciplinary Program of Studies, Research , and Treatment in Nephrology 13 Yeungnam University Hospital , Daegu, Korea 14 Karolinska Institutet 15 Division of Renal Medicine , Clintec, Karolinska Institute , 16 Center of Health and Biological Sciences, Pontifcia Universidade Catlica Do Paran , Curitiba 17 Department of Statistics - Federal University of Juiz de Fora , Juiz de Fora 18 Populations Studies Department , Icbas, University of Porto , Porto, Portugal 19 Yeungnam University Hospital 20 Department of Internal Medicine, Soonchunhyang University Gumi Hospital , Gumi, Korea 21 Department of Internal Medicine, Yeungnam University Hospital , Daegu, Korea 22 Fatih University Medical School Department of Nephrology , Ankara, Turkey 23 Ankara Education and Research Hospital, Department of Internal Medicine , Ankara, Turkey 24 Istanbul University Cerrahpasa Medical Faculty Department of Internal Medicine , Istanbul, Turkey 25 Nuket Bavbek Ruzgaresen 26 Ankara Education and Research Hospital, Department of Nephrology , Ankara, Turkey 27 University Hospital , Zurich, Switzerland 28 Margarete-Fischer-bosch Institute of Clinical Pharmacology , Stuttgart, Germany 29 Robert-Bosch Hospital , Stuttgart, Germany 30 Division of Nephrology, University Hospital , Zurich, Switzerland 31 Department of Mathematics, University of Stuttgart , Germany 32 Beth Israel Deaconess Medical Center , Boston, USA 33 Robert-Bosch Hospital Stuttgart 34 Albert Schweizer Hospital , Dordrecht, The Netherlands 35 Erasmus MC, University Medical Center , Rotterdam, The Netherlands 36 Erasmus MC, University Medical Center , Rotterdam, The Netherlands 37 Department of Internal Medicine, College of Medicine, Yonsei University , Seoul, Korea 38 Department of Internal Medicine, National Health Insurance Corporation Ilsan Hospital , Koyang, Korea , 39 Tokyo Women's Medical University Medical Center East 40 Tokyo Women's Medical University Medical Center East , Tokyo, Japan , 41 Department of Medical 42 Chair and Department of Nephrology Clinic, Jagiellonian University Collegium Medicum , Cracow 43 Chair and Department of Nephrology, Jagiellonian University Collegium Medicum , Cracow, Poland 44 University Hospital Center "Mother Teresa", Department of Nephrology- Dialysis- Transplantation , Tirana, Albania 45 Uhc"Mother Teresa", Department of Nephrology-Dialysis-Transplantation , Tirana, Albania 46 Uhc"Mother Teresa", Department of Statistic , Tirana, Albania 47 Uhc"Mother Teresa",Departemnt of Nephrology-Dialysis-Transplantation,Tirana, Albania 48 Tsukuba University of Technology 49 Namegata District General Hospital 50 University of Tsukuba 51 Yeungnam University Yeongcheon Hospital , Yeongcheon, Korea 52 Nephrology Department, University of Medicine and Pharmacy of Craiova , Romania 53 Clinical Centre of Diabetes, Nutrition, Metabolic Diseases, University of Medicine and Pharmacy of Craiova , Romania 54 Haemodialysis Center, Emergency Hospital Craiova , Romania 55 Gazi University, Medical School, Department of Internal Medicine , Ankara, Turkey 56 Gazi University Medical School, Department of Nephrology , Ankara, Turkey (...truncated)


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Jia Liu, Jia Liu, Yanchun Liu, Yaguang Xu, Xiufen Zhao, Jun Qian, Bin Sun, Changying Xing, Reo Kanda, Chieko Hamada, Takanori Nakano, Keiichi Wakabayashi, Hiroaki Io, Satoshi Horikoshi, Yasuhiko Tomino, Nana Ishimatsu, Tetsu Miyamoto, Hiroyuki Morimoto, Junichi Nakamata, Ryoko Baba, Kaori Kanegae, Ryota Serino, Narutoshi Kabashima, Yutaka Otsuji, Yoshiaki Doi, Masahito Tamura, Junichi Nakamata, Hiroyuki Morimoto, Ryoko Baba, Nana Ishimatsu, Tetsu Miyamoto, Kaori Kanegae, Ryota Serino, Narutoshi Kabashima, Yutaka Otsuji, Yoshiaki Doi, Masahito Tamura, Takuo Kusumoto, Kei Fukami, Sho-Ichi Yamagishi, Seiji Ueda, Yusuke Kaida, Takuma Hazama, Yosuke Nakayama, Ryotaro Ando, Nana Obara, Seiya Okuda, Masahito Tamura, Mika Matsumoto, Tetsu Miyamoto, Kaori Kanegae, Yumi Furuno, Ryota Serino, Narutoshi Kabashima, Yutaka Otsuji, Hsu Bang-Gee, Luisa Mazzotta, Alberto Rosati, Antonio Carlini, Viviana Teixeira Henriques, Edson Zangiacomi Martinez, José Carolino Divino-Filho, Roberto Pecoits-Filho, José Abrão Cardeal Da Costa, Viviana Teixeira Henriques, Viviana Teixeira Henriques, Thiane Gama Axelsson, Bengt Lindholm, Juan Jesus Carrero, Olof Heimburger, Peter Stenvinkel, Abdul Rashid Qureshi, Masanobu Akazawa, Tomomi Uno, Eiichiro Kanda, Yoshitaka Maeda, Maria Aktsiali, Maria Aktsiali, Sofia Antonopoulou, Konstantina Tsiolaki, Neratzoula Bakirtzi, Alexandra Patrinou, Maria Georgopoulou, Paraskevi Liaveri, Nikolaos Afentakis, George Tsirpanlis, Takeshi Hasegawa, Hiroki Nishiwaki, Makoto Hirose, Daisuke Komukai, Hironori Tayama, Fumihiko Koiwa, Ashio Yoshimura, Sing Leung Lui, Sl Lui, Susan Yung, Colin Tang, Flora Ng, Wai Kei Lo, Tak Mao Chan, Hyang Mo Koo, Fa Mee Doh, Dong Eun Yoo, Hyung Jung Oh, Tae-Hyun Yoo, Kyu Hun Choi, Shin-Wook Kang, Dae Suk Han, Seung Hyeok Han, Natalia Fernandes, Natália Fernandes, Marcus Gomes Bastos, Márcia Regina Gianotti Franco, Alfredo Chaoubah, Maria Da Glória Lima, Roberto Pecoits-Filho, José Carolino Divino-Filho, Abdul Rashid Qureshi, Seokhui Kang, Junyoung Do, Kyuhyang Cho, Jongwon Park, Kyungwoo Yoon, , Jin-Bor Chen, Ben-Chung Cheng, Te-Chuan Chen, Yu-Jen Su, Chien-Hsing Wu, , Yongki Park, Jimin Jeon, Maria Tsikeloudi, Panagiotis Pateinakis, Katerina Patsatsi, Eleni Manou, Dimitrios Sotiriadis, Dimitrios Tsakiris, Laetitia Teixeira, Anabela Rodrigues, M.J. Carvalho, António Cabrita, Denisa Mendonça, Seokhui Kang, Junyoung Do, Jongwon Park, Kyuhyang Cho, Kyungwoo Yoon, Maurizio Bruschi, Giovanni Candiano, Laura Santucci, Salvatore Luzio, Rossella Cannavò, Gian Marco Ghiggeri, Enrico Verrina, Yogesh Varadarajan, Balasubramaniyam Raju, Kyu-Hyang Cho, Junyoung Do, Seokhui Kang, Jong-Won Park, Kyung-Woo Yoon, Tae-Woo Kim, Martin Kimmel, Niko Braun, Jörg Latus, Mark Dominik Alscher, Dirk Struijk, Sadie Van Esch, Raymond T. 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Peritoneal dialysis, Nephrology Dialysis Transplantation, 2012, pp. ii457-ii482, 27/suppl 2, DOI: 10.1093/ndt/gfs243