Quantitative LC-MS/MS analysis of seven ginsenosides and three aconitumalkaloids in Shen-Fu decoction

Chemistry Central Journal, Oct 2013

Background Shen-Fu decoction is a traditional Chinese medicine prescription with a 3:2 ratio of Radix Ginseng and Fuzi ( Radix Aconiti lateralis praeparata ). Ginsenosides and alkaloids are considered to be the main active components of Shen-Fu decoction. However, no analytical methods have been used to quantitatively analyse both components in Shen-Fu decoction simultaneously. Results We successfully developed a rapid resolution liquid chromatography coupled with tandem mass spectrometry (RRLC-MS/MS) method for the simultaneous analysis of seven ginsenosides and three aconitum alkaloids in Shen-Fu decoction, the decoction of Radix ginseng and Fuzi ( Radix Aconiti lateralis praeparata ). Chromatogrpahic separation by RPLC was achieved using a reversed-phase column and a water/acetonitrile mobile phase, containing 0.05% formic acid and using a gradient system. The method was optimized to allow for simultaneous analysis of all analytes in 11minutes without the need for baseline resolution of the components. Furthermore, the separation demonstrated good linearity (r > 0.9882), repeatability (RSD < 7.01%), intra- and inter-day precisions (RSD < 5.06%) and high yields of recovery (91.13-111.97%) for ten major constituents, namely ginsenoside-Re, Rg1, Rb1, Rc, Rb2, Rd, Rf, aconitine, hypacoitine and mesaconitine. Conclusions The developed method could be used as a rapid and reliable approach for assessment of the quantity of the major constituents in Shen-Fu decoction.

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Quantitative LC-MS/MS analysis of seven ginsenosides and three aconitumalkaloids in Shen-Fu decoction

Chemistry Central Journal Quantitative LC-MS/MS analysis of seven ginsenosides and three aconitum alkaloids in Shen-Fu decoction Na Guo Mingtao Liu Dawei Yang Ying Huang Xiaohong Niu Ruifan Wu 1 Ying Liu Guizhi Ma 1 Deqiang Dou 0 0 Department of Chinese Medicine Chemistry, Liaoning University of Traditional Chinese Medicine , Dalian 116600 , China 1 College of Pharmacy, Xinjiang Medical University , Urumqi 830011 , China Background: Shen-Fu decoction is a traditional Chinese medicine prescription with a 3:2 ratio of Radix Ginseng and Fuzi (Radix Aconiti lateralis praeparata). Ginsenosides and alkaloids are considered to be the main active components of Shen-Fu decoction. However, no analytical methods have been used to quantitatively analyse both components in Shen-Fu decoction simultaneously. Results: We successfully developed a rapid resolution liquid chromatography coupled with tandem mass spectrometry (RRLC-MS/MS) method for the simultaneous analysis of seven ginsenosides and three aconitum alkaloids in Shen-Fu decoction, the decoction of Radix ginseng and Fuzi (Radix Aconiti lateralis praeparata). Chromatogrpahic separation by RPLC was achieved using a reversed-phase column and a water/acetonitrile mobile phase, containing 0.05% formic acid and using a gradient system. The method was optimized to allow for simultaneous analysis of all analytes in 11minutes without the need for baseline resolution of the components. Furthermore, the separation demonstrated good linearity (r > 0.9882), repeatability (RSD < 7.01%), intra- and inter-day precisions (RSD < 5.06%) and high yields of recovery (91.13-111.97%) for ten major constituents, namely ginsenoside-Re, Rg1, Rb1, Rc, Rb2, Rd, Rf, aconitine, hypacoitine and mesaconitine. Conclusions: The developed method could be used as a rapid and reliable approach for assessment of the quantity of the major constituents in Shen-Fu decoction. Ginsenosides; Aconitum alkaloids; Shen-Fu decoction; RRLC-MS/MS - Background Decoction is the traditional prescription of traditional Chinese medicines (TCMs). Based on TCM theory, one single herb or several kinds of herbs combined are boiled in water to make the decoction. First documented in 1465, Shen-Fu decoction is a TCM prescription with a 3:2 ratio of Radix Ginseng and Fuzi (Radix Aconiti lateralis praeparata). Both components have been commonly used as herbal medicines in China for about 1800 years, predominantly used for folk treatment of diseases with the sign of Yangqi decline or Yang exhaustion. Shen-Fu decoction is also used to treat cardiovascular diseases such as circulatory collapse, shock, thoracic obstruction and acute thoracic pain. Shen-Fu Injection (SFI for intravenous medication), is a typical form of Shen-Fu decoction, that has been used for treatment of many kinds of diseases because of its cardiovascular protective effectiveness [1-3]. The main active components found in Shen-Fu decoction are ginsenosides and alkaloids. Ginsenosides are generally classified into four groups: protopanaxadiol, protopanaxatriol, ocotillol and oleanolic acid type [4-6], Currently, more than 150 ginsenosides have been isolated and identified in the literature. Among them, ginsenosides-Rb1, Rb2, Rc, Rd, Rg1, Re and Rf (Figure 1) are the most important compounds in chemical analysis of ginsengs. At present, about 224 alkaloids have been isolated and identified from Aconitum [7,8]. -O-Glc6-1Glc -O-Glc6-1Arap -O-Glc6-1Araf -O-Glc -O-Glc2-1Rha -O-Glc -O-Glc2-1Glc Protopanaxadiol-type Ginsenoside-Rb1 Ginsenoside-Rb2 Ginsenoside-Rc Ginsenoside-Rd Protopanaxadiol-type Ginsenoside-Re Ginsenoside-Rg1 Ginsenoside-Rf -O-Glc2-1Glc -O-Glc2-1Glc -O-Glc2-1Glc -O-Glc2-1Glc Figure 1 Chemical structures of ginsenosides and Aconitum alkaloids analyzed in Shen-Fu decoction. These have been classified into four major groups, nonester alkaloids (NEAs), monoester diterpene alkaloids (MDAs), diester diterpene alkaloids (DDAs) and lipoalkaloids. Aconitum alkaloids are mainly constituted of three DDAs, diester-diterpence called aconitine (AC), measaconitine (MA) and hypaconitine (HA) (Figure 1). They are known for their high toxicity and pharmacological activity, as well as being the target markers of Fuzi. In general, the curative effect of traditional Chinese medicine is an integrative result of a number of ginsenosides and alkaloids. In order to minimize the variability of active ingredients in the decoction and ensure repeatable and reproducible therapeutic effects, it is very important to establish quality control methodology for the decoction. To this end, analysis of ginsenosides and Aconitum alkaloids is required to assess the quality of Shen-Fu decoction. Previous methods that have been used to analyze ginsenosides and alkaloids include HPLC-DAD (ELSD), CE, GC-MS and LC-MS [9-19] and alkaloids [20-30]. In comparison with traditional HPLC, RRLC provides a higher peak capacity, greater resolution, increased sensitivity and higher speed o (...truncated)


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Na Guo, Mingtao Liu, Dawei Yang, Ying Huang, Xiaohong Niu. Quantitative LC-MS/MS analysis of seven ginsenosides and three aconitumalkaloids in Shen-Fu decoction, Chemistry Central Journal, 2013, pp. 165, Volume 7, Issue 1, DOI: 10.1186/1752-153X-7-165