Association of genetic loci for migraine susceptibility in the she people of China

The Journal of Headache and Pain, Aug 2015

Background The purpose of this study was to investigate the association of the genotype and allele frequencies of the polymorphisms rs4379368, rs10504861, rs10915437, rs12134493 and rs13208321 in She people of China with migraine headache susceptibility. The five alleles were previously identified as being associated with migraine in a Western population, but it was not known if this association would hold in a She population. rs4379368 is in the succinic HMG coenzyme A transferase (C7orf10) gene; rs10504861 is near the matrix metallopeptidase 16 (MMP16) gene; rs10915437 is near the adherens junctions associated protein 1 (AJAP1) gene; rs12134493 is upstream of the tetraspanin 2 (TSPAN2) gene; and rs13208321 is within the four and a half LIM domains protein 5 (FHL5) gene. Methods This was a case-controlled study conducted in She people of Fujian province in China. Polymerase chain reaction-restriction fragment length polymorphism and direct sequencing were performed. Univariate and multivariate analyses were used to assess the association of the different genotypes of each SNP with migraine. Results The rs4379368 T allele was not in Hardy-Weinberg equilibrium and was more common than the C allele in subjects with migraine (58.7 %; P = 0.049), possibly suggesting a selection bias for T allele in this population. In support of this, the CT and TT genotypes were more frequent in the migraine compared with the control groups (54.0 % and 31.7 % vs. 48.0 % and 28.7 %, respectively; P = 0.019). These genotypes were also more common in females with migraines than females without migraines (53.8 % and 30.9 % vs. 46.7 % and 27.6 %; P = 0.026). Univariate and multivariate analyses found the CC genotype of rs4379368 and AA or AG genotype of rs13208321 were associated with a reduced risk of migraine (P values ≤0.039). Conclusions Our findings suggest that rs4379368 and rs13208321 are potential genetic markers for migraine in this She population. The findings of this study and others indicate important differences between ethnic populations in regard to genetic markers of migraine susceptibility.

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Association of genetic loci for migraine susceptibility in the she people of China

Lin et al. The Journal of Headache and Pain Association of genetic loci for migraine susceptibility in the she people of China Qi-Fang Lin 0 Xian-Guo Fu 1 Long-Teng Yao 0 Jing Yang 1 Luo-Yuan Cao 1 Yong-Tong Xin 0 Jun-Xia Hou 0 Lin-Feng Ye 0 Gen-Bin Huang 0 0 Department of Internal Neurology, Ningde Municipal Hospital, Affilliated Hospital of Fujian Medical University , Jiaocheng District, Ningde City, Fujian 352100 , China 1 Laboratory of Cell and Molecular Biology, Ningde Municipal Hospital, Affilliated Hospital of Fujian Medical University , Ningde, Fujian , China Background: The purpose of this study was to investigate the association of the genotype and allele frequencies of the polymorphisms rs4379368, rs10504861, rs10915437, rs12134493 and rs13208321 in She people of China with migraine headache susceptibility. The five alleles were previously identified as being associated with migraine in a Western population, but it was not known if this association would hold in a She population. rs4379368 is in the succinic HMG coenzyme A transferase (C7orf10) gene; rs10504861 is near the matrix metallopeptidase 16 (MMP16) gene; rs10915437 is near the adherens junctions associated protein 1 (AJAP1) gene; rs12134493 is upstream of the tetraspanin 2 (TSPAN2) gene; and rs13208321 is within the four and a half LIM domains protein 5 (FHL5) gene. Methods: This was a case-controlled study conducted in She people of Fujian province in China. Polymerase chain reaction-restriction fragment length polymorphism and direct sequencing were performed. Univariate and multivariate analyses were used to assess the association of the different genotypes of each SNP with migraine. Results: The rs4379368 T allele was not in Hardy-Weinberg equilibrium and was more common than the C allele in subjects with migraine (58.7 %; P = 0.049), possibly suggesting a selection bias for T allele in this population. In support of this, the CT and TT genotypes were more frequent in the migraine compared with the control groups (54.0 % and 31.7 % vs. 48.0 % and 28.7 %, respectively; P = 0.019). These genotypes were also more common in females with migraines than females without migraines (53.8 % and 30.9 % vs. 46.7 % and 27.6 %; P = 0.026). Univariate and multivariate analyses found the CC genotype of rs4379368 and AA or AG genotype of rs13208321 were associated with a reduced risk of migraine (P values ≤0.039). Conclusions: Our findings suggest that rs4379368 and rs13208321 are potential genetic markers for migraine in this She population. The findings of this study and others indicate important differences between ethnic populations in regard to genetic markers of migraine susceptibility. Migraine; Single-nucleotide polymorphism; Susceptibility; She people; rs13208321; rs13208321 - Background Migraine headache refers to a common, chronic, and recurrent headache that significantly impacts the ability to work, results in greater health care resource utilization and cost, and a reduced quality of life [1, 2]. A survey study on migraine, conducted in USA in 2010, showed the prevalence of migraine was 13.2 % in the total population, 8.6 % in males, and 17.5 % in females [3]. The etiology and pathogenesis of migraine are still poorly understood [4]. Epidemiological studies found that there is a genetic and familial predisposition for migraine [5–7]. We previously performed an epidemiological study in the She ethnic minority population from Fujian province in China [5]. She people have their own special lifestyle, cultural heritage, and genetic background, which are different from those of Han people and other Asians. The study was a cross-sectional survey in which we evaluated the prevalence of migraine within the She population. We found the prevalence of migraine was about 10.5 %. Recently, common genetic variants were identified that are associated with migraine susceptibility through twin, family clustering, and genome wide association studies of patients with migraine (with and without aura) [8, 9]. Several genome-wide association studies have investigated risk variants associated with migraines [10, 11]. Recently, Anttila et al. [11] conducted a large migraine meta-analysis that included 29 studies and 23285 patients with migraines and 95425 controls. They identified 12 single nucleotide polymorphisms (SNPs) that were associated with migraine susceptibility. Five of these SNPs had never previously been associated with the condition. They are: rs4379368 located in an intron of the succinic HMG coenzyme A transferase (C7orf10) gene; rs10504861 located close to the matrix metallopeptidase 16 (MMP16) gene; rs10915437 positioned in the vicinity of the adherens junctions associated protein 1 (AJAP1) gene; rs12134493 located upstream of the tetraspanin 2 (TSPAN2) gene; and rs13208321 located within the four and a half LIM domains protein 5 (FHL5) gene. To date only one study performed in a Spanish population has further tested the association o (...truncated)


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Qi-Fang Lin, Xian-Guo Fu, Long-Teng Yao, Jing Yang, Luo-Yuan Cao, Yong-Tong Xin, Jun-Xia Hou, Lin-Feng Ye, Gen-Bin Huang. Association of genetic loci for migraine susceptibility in the she people of China, The Journal of Headache and Pain, 2015, pp. 70, Volume 16, Issue 1, DOI: 10.1186/s10194-015-0553-1