Relationship between renal function and circulating microparticles, soluble P-selectin and E-selectin levels in atrial fibrillation

Journal of Thrombosis and Thrombolysis, Sep 2016

Atrial fibrillation (AF) and chronic kidney disease are closely related, and any associated risk of stroke and thromboembolism due to AF is increased by concurrent renal dysfunction. The mechanism(s) for this include abnormalities in platelets and endothelial cells. We hypothesized relationships between levels of circulating platelet microparticles (PMPs, defined by CD42b), soluble P selectin (both reflecting platelet activation), soluble E-selectin (reflecting endothelial activation) and endothelial/platelet microparticles (EPMPs, defined by CD31) with progressive renal dysfunction. Blood samples were obtained from 160 anticoagulated AF patients. Microparticles were measured by flow cytometry, soluble E and P selectin levels by ELISA. Renal function was determined by estimated glomerular filtration rate (eGFR). EPMP levels demonstrated a linear increased trend across quartiles of eGFR (p = 0.034) and CKD stage (p < 0.001), and correlated with eGFR and serum creatinine (p < 0.01). PMPs, P-selectin and E-selectin levels were not significantly different across groupings of renal dysfunction, and no significant correlations with eGFR were evident (p = 0.186, p = 0.561, p = 0.746 respectively). Stepwise multivariable regression analysis demonstrated that worsening renal function was an independent predictor of EPMP levels (p < 0.001). In well-anticoagulated AF patients, there is potential relationship between endothelial function (as judged by elevated EPMP levels, with no change in PMPs) and renal function. Other markers of prothombotic state or cellular activation (PMP, P-selectin and E-selectin levels) were not significantly different across the various degree of renal dysfunction. Renal function must be addressed when measuring EPMP levels.

A PDF file should load here. If you do not see its contents the file may be temporarily unavailable at the journal website or you do not have a PDF plug-in installed and enabled in your browser.

Alternatively, you can download the file locally and open with any standalone PDF reader:

Relationship between renal function and circulating microparticles, soluble P-selectin and E-selectin levels in atrial fibrillation

eRlationshp ebtwn neal r ufnctio nad luatng icr poatles, imcr oslube -Pselctin nad -Eselctin elvs ni taril we Andr geoy Gr - D O . 1 0 1 4 2 7 ,12 1 1 Y .D . Y .H P S 0216. A A F A F ydsfunctio. P E P M P C D 4 2 , P A · C · , E M · S P · S E E P C D 3 1 ydsfunctio. A F M , E P A F ) G F R E P M P G F R .034) .01), = < ned-stag .01) MPs, -Pselc G F E A F .0186, .0561, G F = = aptiens .0746 = .01) nI A F Y .H hTus, rent dif cSien, oRad, KU C D T S A H lAthoug P ta 31,0 g htob s etalp ,macbA( ,egdirbmaC )KU dnoces ucni saw ylt morp d m ofrep ,0 1 , 02 dna naticogul w(arfin) ofr ta elast 21 ewks. oDsedajustmen ofr awrfin aws odne ni pseciald csient1 μ m d i a m e t e r ( A p o g e e F l o w S y s t e m s ) w e r e u s e d t o s e t u p eld naticogul lcins, cahievng a tsable niteraol t h e M P NIR aws edtrmin no hte ady fo etsing ot sae tciv ef naticogul. F o r e n z y m e morf n it e loc gae 81 eyars, rof .C° ehT natluser msalp litnu retal hctab gni secorp rusaem e bulos nitc les-E hcroni ilver ocr tSaiscl ercnt ahemo oCntiusly avrible adt rae xeprsd sa eman nad tsan p h i l i a o r t h r o m b o p h i l i a ( s u c h a s F a c t o r nad r e s e a r c h i n d i c e s , d a t a w e r e g r o u p e d b y q u a r t i l e o f e G F R a n d laso yb hte KCD tsage, hta ebing tSage 1 A orutine G F R ) S t a g e 2 ( e G F R 6 0 – 8 9 ) S t a g e ≥ usbeqnt 92) [ 2 .] nI caordne iwth is fo avrince fo rgoups hta rae nidept), ubt nistead ocnsiderat hsould eb igven hwetr hter si a ilnear rtend niformed caros hte ofur rgoups. hTis aws osught caording ot emth botained rfom nao dos edscrib yb lAtman [ 32 ] G F R h a s a anturl ocntius avriton, ocrelatins ewr laso osught ebtwn ernal nad imcropatles, nad .8 oNmura S 2(016) iMcropatle nad taherombic idsea. J m a y b e d i f f i c u l t , a n d w apth hter si htem ersitan ot idsocatn. J rfom oBehring ofr 41. mAabile ,N uGerin , PA degui T ,A oBulanger MC, oLnd MG avlue fo hCest idstrbuon, nad erpoductin ni nay emdiu, rpovide oyu igve . J e s e l L , A b b a s M , T o t i F , C o h e n A , A r e n t z T , M o r e l O ( 2 0 1 3 ) ilnk ot hte rCeativ oCmns ilcens, nad nidcate fi hcanges ewr M i c r o p a r t i c l e s i n a t r i a l f i b r i l l a t i o n : a l i n k b e t w e e n c e l l a c t i v a t i o n amde. ro paotsi, itsue ermodling nad . thrombgenicy nIt J aCr idol 618(2):0–9 71. lBan DA, aNdr KS, iLp YG 2(03) hTe , Y ledry f i c a t i o n o f d i e t i n r e n a l d i s e a s e s t u d y g r o u p otwards tsandrizo L te la 2(09) J hTromb hte vealution nad amnget fo BJ, iLp , LA, eny nIt S(upl )3:1–50 e t a l ( 2 0 1 2 ) J eMd 637:25– .5 NA, iLp , LA, RP, aLmberts ,M oHm J mA , oBdaert avsculr idsea ni aptiens iwth :)38– 16( 2 5 M o b a r r e z F J a c o b s o n S j e m d a h l P 2(016) cets Ef fo ilpd-owerng rteamn no oMntr-Gaci ,S , hTromb 3 b a r r i e r f u n c t i o n b y a n t i b o d y d r i v e n a f f i n i t y m o d u l a t i o n o f p l a t e l e t e c u l e imcropatles m c o a t c e i n n a v l r t i l i r l a i n . J C r i l 63. 73. iwth dEerhy ratey iSng alte eBrnal-Mizch amn saocited idac aCine lPate yhpertnsiv aNdr uDrsn mA iwth uOtcomes ,N iCrculatng ni iwth 415:962–70 idsea. ictraed acrdiovsul erliab nidexs lBan iral T hcroni , W ystemic yHpertns uEr ont iwth erlation nAgelatoi ikdney 2(03) laent W saculr J ratey 02(9):57–64 J V eHart hAlers caute ufnctio nUsur aKmth oSlube hte iBol leasticy idsea , P ocrnay iHgh E aPstor aMlt :5267– nad 23:04–1 rB oKsil aptiens eLvls hsould aCr elvs 2(015) eBvrs iwth + iNckeng (42):15– aMuro yDnamics usb uHgel eHart iNckeng ocrnay ,G ML, admge aHemtol emasurd icrulatng iLp V ,B G oHrst nedothlia icrulatng rpocagulnt YG imc N 2(06) aJnower + A/nexi imc ocrelat aLu .3 3 nag W eMi osn iBochemal uhman xEp eMtzlar lAteraion eKlrmai eKlrmai rPivatsky htelia la uHang acrdioenl icrulatng fo hCen iwth eCl JP lpate aPdock 2(013) lAias hZang P(ECAM-1). C(D31 lace W eRs EPCAM-1: eLrtkiamongl fo nIt , nag W nedothlia imcropa a u r e , , , , 83. 93. 04.

This is a preview of a remote PDF:

Yee Cheng Lau, Qinmei Xiong, Andrew D. Blann, Gregory Y. H. Lip. Relationship between renal function and circulating microparticles, soluble P-selectin and E-selectin levels in atrial fibrillation, Journal of Thrombosis and Thrombolysis, 2017, 18-23, DOI: 10.1007/s11239-016-1427-3