Study of Holtermanniella wattica, Leucosporidium creatinivorum, Naganishia adeliensis, Solicoccozyma aeria, and Solicoccozyma terricola for their lipogenic aptitude from different carbon sources

Biotechnology for Biofuels, Nov 2016

Background The ability of some microorganisms to accumulate lipids is well known; however, only recently the number of studies on microbial lipid biosynthesis for obtaining oleochemical products, namely biofuels and some building blocks for chemistry, is rapidly and spectacularly increased. Since 1990s, some oleaginous yeasts were studied for their ability to accumulate lipids up to 60–70% of their dry weight. Due to the vast array of engineering techniques currently available, the recombinant DNA technology was the main approach followed so far for obtaining lipid-overproducing yeasts, mainly belonging to the Yarrowia lipolytica. However, an alternative approach can be offered by worldwide diversity as source of novel oleaginous yeasts. Lipogenic aptitude of a number of yeast strains has been reviewed, but many of these studies utilized a limited number of species and/or different culture conditions that make impossible the comparison of different results. Accordingly, the lipogenic aptitude inside the yeast world is still far from being fully explored, and finding new oleaginous yeast species can acquire a strategic importance. Results Holtermanniella wattica, Leucosporidium creatinivorum, Naganishia adeliensis, Solicoccozyma aeria, and Solicoccozyma terricola strains were selected as a result of a large-scale screening on 706 yeasts (both Ascomycota and Basidiomycota). Lipid yields and fatty acid profiles of selected strains were evaluated at 20 and 25 °C on glucose, and on glycerol, xylose, galactose, sucrose, maltose, and cellobiose. A variable fatty acid profile was observed in dependence of both temperature and different carbon sources. On the whole, L. creatinivorum exhibited the highest performances: total lipid yield (Y L ) >7 g/l on glucose and glycerol, % of intracellular lipids on cell biomass (Y L /DW) >70% at 20 °C on glucose, lipid coefficient (Y L /Glu) around 20% on glucose, and daily productivity (Y L /d) on glucose and sucrose >1.6 g/(l*d). Conclusions This study provides some meaningful information about the lipogenic ability of some yeast species. Variable lipid yields and fatty acid profiles were observed in dependence of both temperature and different carbon sources. L. creatinivorum exhibited the highest lipogenic performances.

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Study of Holtermanniella wattica, Leucosporidium creatinivorum, Naganishia adeliensis, Solicoccozyma aeria, and Solicoccozyma terricola for their lipogenic aptitude from different carbon sources

Filippucci et al. Biotechnol Biofuels Study of Holtermanniella wattica, Leucosporidium creatinivorum, Naganishia adeliensis, Solicoccozyma aeria, and Solicoccozyma terricola for their lipogenic aptitude from different carbon sources Sara Filippucci 0 3 Giorgia Tasselli 0 3 Alessandro Scardua 2 Simone Di Mauro 0 3 Maria Rita Cramarossa 1 Davide Perini 2 Benedetta Turchetti 0 3 Andrea Onofri 0 3 Luca Forti 1 Pietro Buzzini 0 3 0 Department of Agricultural, Food and Environmental Sciences & Industrial Yeasts Collection DBVPG, University of Perugia , Borgo XX Giugno 74, 06121 Perugia , Italy 1 Department of Life Sciences, University of Mod- ena and Reggio Emilia , via Campi 287, Modena 41125 , Italy 2 Laboratories of Biotechnology , Novamont S.p.A, via Fauser 8, Novara 28100 , Italy 3 Department of Agricultural, Food and Environmental Sciences & Indus- trial Yeasts Collection DBVPG, University of Perugia , Borgo XX Giugno 74, 06121 Perugia , Italy Background: The ability of some microorganisms to accumulate lipids is well known; however, only recently the number of studies on microbial lipid biosynthesis for obtaining oleochemical products, namely biofuels and some building blocks for chemistry, is rapidly and spectacularly increased. Since 1990s, some oleaginous yeasts were studied for their ability to accumulate lipids up to 60-70% of their dry weight. Due to the vast array of engineering techniques currently available, the recombinant DNA technology was the main approach followed so far for obtaining lipid-overproducing yeasts, mainly belonging to the Yarrowia lipolytica. However, an alternative approach can be offered by worldwide diversity as source of novel oleaginous yeasts. Lipogenic aptitude of a number of yeast strains has been reviewed, but many of these studies utilized a limited number of species and/or different culture conditions that make impossible the comparison of different results. Accordingly, the lipogenic aptitude inside the yeast world is still far from being fully explored, and finding new oleaginous yeast species can acquire a strategic importance. Results: Holtermanniella wattica, Leucosporidium creatinivorum, Naganishia adeliensis, Solicoccozyma aeria, and Solicoccozyma terricola strains were selected as a result of a large-scale screening on 706 yeasts (both Ascomycota and Basidiomycota). Lipid yields and fatty acid profiles of selected strains were evaluated at 20 and 25 °C on glucose, and on glycerol, xylose, galactose, sucrose, maltose, and cellobiose. A variable fatty acid profile was observed in dependence of both temperature and different carbon sources. On the whole, L. creatinivorum exhibited the highest performances: total lipid yield (YL) >7 g/l on glucose and glycerol, % of intracellular lipids on cell biomass (YL/DW) >70% at 20 °C on glucose, lipid coefficient (YL/Glu) around 20% on glucose, and daily productivity (YL/d) on glucose and sucrose >1.6 g/(l*d). Conclusions: This study provides some meaningful information about the lipogenic ability of some yeast species. Variable lipid yields and fatty acid profiles were observed in dependence of both temperature and different carbon sources. L. creatinivorum exhibited the highest lipogenic performances. Oleaginous yeasts; Lipid yield; Fatty acid profile; Large-scale screening; Leucosporidium creatinivorum - Background The ability of some microorganisms to accumulate high intracellular amounts of lipids has been known for decades; however, only recently the number of studies on microbial lipid biosynthesis for obtaining oleochemical products, namely biofuels and some building blocks for lubricants, adhesives, solvents, biosurfactants, cosmetics, and degradable polymers, has rapidly and spectacularly increased, becoming a growing part of the so-called “white biotechnology” [1–4]. In particular, the production of vegetable and microbial lipids is largely connected to the advent of biodiesel, which is considered one of the most promising biofuels [1–3]. Biodiesel is derived by homogeneously or heterogeneously catalyzed transesterifications of triacylglycerols (TAGs). The increasingly global request of biodiesel from vegetable oils occurred from early 2000s, and it has caused the dramatic increase of market prices for a number of foodstuffs. Moreover, vegetable lipids are considered one of the most important renewable feedstocks of the chemical industry since they could be processed by means of chemical routes and/or biotechnology approaches to produce high-value chemical compounds and non-fuel oil-derived products [5–7]. Accordingly, finding new biological sources of lipids acquires a strategic importance to reduce (or even to avoid) any competition with food resources [8, 9]. In this framework, microbial lipids are among the most promising feedstock sources for oil production because their composition is quite similar to that found in most vegetable oils [10]. Besides, taking into consideration the possibility to store (...truncated)


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Sara Filippucci, Giorgia Tasselli, Alessandro Scardua, Simone Di Mauro, Maria Cramarossa, Davide Perini, Benedetta Turchetti, Andrea Onofri, Luca Forti, Pietro Buzzini. Study of Holtermanniella wattica, Leucosporidium creatinivorum, Naganishia adeliensis, Solicoccozyma aeria, and Solicoccozyma terricola for their lipogenic aptitude from different carbon sources, Biotechnology for Biofuels, 2016, pp. 259, 9, DOI: 10.1186/s13068-016-0672-1