Synthesis and characterisation of zinc oxide nanoparticles using terpenoid fractions of Andrographis paniculata leaves

International Nano Letters, Apr 2017

Zinc oxide (ZnO) nanoparticles have been widely employed for various pharmacological applications. Several approaches were tried to synthesize ZnO nanoparticles. In this study, ZnO nanoparticles were biosynthesized using terpenoid (TAP) fractions isolated from Andrographis paniculata leaves. Subsequently, the ZnNO3 (0.1 N) is treated with the isolated TAP fractions to biosynthesize zinc oxide nanoparticles (Zn-TAP NPs). This nanoparticle preparation has been confirmed by the colour change from green to cloudy-white and the peak at 300 nm by UV–Visible spectra. FTIR analysis of Zn-TAP NPs showed the presence of functional group (i.e.) C=O which has further been confirmed by H1-NMR studies. From SEM and XRD analysis, it has been found that the hexagonal nanorod particle is 20.23 nm in size and +17.6 mV of zeta potential. Hence, it can be easily absorbed by negatively charged cellular membrane to contribute for efficient intracellular distribution. Therefore, it is suggested that the synthesised Zn-TAP NPs are more suitable in drug delivery processes.

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Synthesis and characterisation of zinc oxide nanoparticles using terpenoid fractions of Andrographis paniculata leaves

Synthesis and characterisation of zinc oxide nanoparticles using terpenoid fractions of Andrographis paniculata leaves S. Kavitha 0 1 2 3 M. Dhamodaran 0 1 2 3 Rajendra Prasad 0 1 2 3 M. Ganesan 0 1 2 3 0 Department of Chemistry (R&DC), Bharathiar University , Coimbatore, Tamil Nadu , India 1 A.V.C. Polytechnic College , Mannampandal, Mayiladuthurai, Nagai (DT), Tamil Nadu 609305 , India 2 Department of Biochemistry and Biotechnology, Annamalai University , Chidambaram, Tamil Nadu , India 3 Department of Chemistry, Perunthalaivar Kamarajar Institute of Engineering and Technology (PKIET) (Government of Puducherry Institution) , Karaikal, Puducherry U.T. , India Zinc oxide (ZnO) nanoparticles have been widely employed for various pharmacological applications. Several approaches were tried to synthesize ZnO nanoparticles. In this study, ZnO nanoparticles were biosynthesized using terpenoid (TAP) fractions isolated from Andrographis paniculata leaves. Subsequently, the ZnNO3 (0.1 N) is treated with the isolated TAP fractions to biosynthesize zinc oxide nanoparticles (Zn-TAP NPs). This nanoparticle preparation has been confirmed by the colour change from green to cloudy-white and the peak at 300 nm by UV-Visible spectra. FTIR analysis of Zn-TAP NPs showed the presence of functional group (i.e.) C=O which has further been confirmed by H1-NMR studies. From SEM and XRD analysis, it has been found that the hexagonal nanorod particle is 20.23 nm in size and ?17.6 mV of zeta potential. Hence, it can be easily absorbed by negatively charged cellular membrane to contribute for efficient intracellular distribution. Therefore, it is suggested that the synthesised Zn-TAP NPs are more suitable in drug delivery processes. Terpenoids paniculata; ZnNO3; Nanoparticles; Andrographis; Zinc oxide nanoparticles - Nanotechnology is an upcoming field of science which has its impact in various fields such as energy, environment, electronics, etc. The widespread practical applications of metal nanoparticles (particles less than 100 nm) are attributed to their unique properties [1]. Different physical and chemical processes are widely used to synthesize metal nanoparticles [2]. However, these production methods are usually expensive, labor-intensive, and are potentially hazardous to the environment and living organisms [3]. Thus, there is an obvious need for an alternative, costeffective and at the same time, safe and environmentally sound method of nanoparticles production [4]. During the past decades, it has been demonstrated that many biological systems, including plants and bacteria [5] and fungi [6] can transform inorganic metal ions into metal nanoparticles via the reductive capacities of the proteins and metabolites present in these organisms. The ability of plant extracts to reduce metal ions has been known since the early 1900s, although the nature of the reducing agents involved was not well understood. Because of its simplicity, the use of live plants or whole plant extracts and plant tissue for reducing metal salts to nanoparticles has attracted considerable attention within the last 30-years [7]. Plant extracts act both as reducing and stabilizing agents in the synthesis of nanoparticles [8]. The source of the plant extract is known to influence the characteristics of the nanoparticles [9]. This is because different extracts contain different concentrations and combinations of organic reducing agents [10]. Typically, a plant extract-mediated bioreduction involves mixing aqueous extract with an aqueous solution of the relevant metal salt. The reaction occurs at room temperature and is generally completed within a few minutes. Due to the number of different chemicals involved, the bioreduction process is relatively complex. Nanoparticles have gained importance due to the awareness in biological processes. The chemicals present in the plant with anti-oxidant property are the basis for the preparation of zinc oxide nanoparticle. It has become a necessity to develop nanoparticles which in turn can be targeted on different applications [11]. Nanoparticles of zinc oxide are under intensive study for their applications in the field of optical devices, catalysis, biotechnology, DNA labeling, drugs and medical and chemical sensors. Nanosized zinc oxide has found various applications (sunscreen coatings and paints) due to its high absorption in UV [12, 13]. Currently, researchers are focusing on the synthesis of nanoparticles using green methods. Synthesis of nanoparticles using green methods increases the biological effectiveness. Bio-nanoparticles have greater catalytic activity due to the increase in the surface area. The possibility of using plant materials as nano-precursors has also been studied. The plant species A. paniculata, commonly known as Nilavembu in India, belongs to the Acanthaceae family. It is found in a large extent throughout South China, Asian countries and Sri Lanka. It is also known as ‘‘King of bitters’’ [14, 15]. Despite its bitter taste, this species possesses pharmacological properties, i.e. antimicrobial, antioxidant, antiinflammatory, antiparasitic, antihyperglycemic, hypoglycemic and antiallergic [16]. Andrographis paniculata reduces oxidation level due to its steroidal characteristics and destroys infected somatic cells. It contains diterpenes, lactones and flavonoids. The leaf and stem extracts have glycosides, flavonoids, gums, steroids, terpenoids, tannins, saponins and phenolic compounds [17]. Compared to its other parts, the leaf, which has multiple clinical applications, has huge amounts of terpenoid (TAP) (2.39%) which accounts for the bitter taste in leaves. This reason has been an impetus to isolate the medically most active and major compound TAP from the leaf. Therefore, in this study TAPs were isolated from A. paniculata and used to prepare ZnO nanoparticles for possible pharmacological applications. Materials and methods The plant A. paniculata was collected from the campus of A.V.C. Arts and Science College, Mayiladuthurai. The AR grade chemicals and solvents like zinc nitrate tetrahydrate, sodium hydroxide, ethanol, chloroform, silica gel and CDCl3 were procured from Merck Chemicals, Pune, India. Extract preparation The collected A. paniculata leaves were cleaned with tap water then rinsed with distilled water, dried, cut into small parts and ground into fine powder. It was further stored at 37 C. The terpenoid fractions were separated by column chromatography. In this method, 25 g silica gel powder was filled in column apparatus and mixed with ethanol up to slurry formation. Then the solvent was completely eluted, and added 50% mixture (65 mL CHCl3 and 1 mL methanol). It was followed by adding 10 mL ethanolic sample. Finally, the remaining mixture was added. The solvent was eluted. Within 12 h terpenoid was collected in test tubes. Phytochemical test for terpenoid (TAP) fractions from A. paniculata Confirmative test for terpenoids Salkowskis test TAP was mixed with a few drops of chloroform and concentrated sulphuric acid. The formation of yellow colour indicates the presence of terpenoids. The extract was treated with 1 mL of CHCl3, 1 mL CH3COOH and few drops of concentrated sulphuric acid. Appearance of brown ring indicates the presence of terpenoids. Synthesis of zinc oxide nanoparticles (Zn-NPS) Zinc oxide nanoparticles were prepared by green synthesis (co-precipitation) method. 0.1 N aqueous solution of zinc Fig. 1 TAP mediated Zn-TAP NPs synthesis Fig. 2 Schematic diagram of Zn-TAP NPs synthesis Vigorous stirring for 10 mts and At 60o C for 3 hrs by adding 0.1 N NaOH in time Cloudy- White precipitate (Zn- nanopar cles) nitrate tetrahydrate (Zn (NO3)2 4H2O) was added to 50 mL distilled water by continuous shaking. Later 0.1 N NaOH was added in 10 m gap for an hour. Following that, the time gap was increased for adding the NaOH. The procedure was repeated for 2 h. The obtained white solution was stirred for 2 h at pH 12. The product was washed with distilled water and ethanol to get the final product. It is then dried overnight. The whole mode of proposed method for the synthesis of Zn-NPs mediated by the aqueous extract was illustrated. Characterisation of terpenoid zinc oxide nanoparticles (Zn-TAP NPs) from A. paniculata Particle size and zeta potential measurements DLS and zeta potential were based on the direction and velocity of particles under the influence of known electric field. Malvern Zetasizer ZS (Malvern Instruments, Malvern, UK) instrument was used to measure particle size, size distribution and zeta potential of Zn-TAP NPs. To suit the above situation a homogenous suspension was created using the lyophilized nanoparticles in double distilled water and repeated thrice. Scanning electron microscopy (SEM) The samples were placed on a carbon plated platinum strip. Splash drops were wiped off. It was dried in mercury lamp for 5 m and examined under SEM (using JEOL JSM-6610 LV SEM machine). UV spectroscopic analysis The Zn-TAP NPs were dissolved in distilled water (1 mg/ mL) and scanned in a Perkin Elmer Lambda 25 UV–Vis spectrometer at 25 C in the range of 250–650 nm. The UV spectrum was repeated three times. Fourier transform infrared spectroscopy (FTIR) analysis Zn-TAP NPs and potassium bromide 10 and 100 mg were respectively mixed to form a salt plate. Spectra between 4000 and 400 cm-1 were noted in a Bio-Rad FTIR–40 (USA). Proton nuclear magnetic resonance spectroscopy (1H-NMR) analysis An approximately 30 mg of Zn-TAP NPs was made soluble in 0.5 mL CDCl3 (99.9%). At 27 C 1H-NMR spectra were recorded by Luo and Fan method (2011). Green synthesis of zinc oxide nanoparticles The biosynthesised Zn-TAP NPs were isolated (Fig. 1) using 0.1 N Zinc nitrate with NaOH of co-precipitation method at pH 12 (Fig. 2). Characterisation of Zn-TAP NPs from A. paniculata X-ray diffraction (XRD) analysis Particle size and zeta potential of Zn-TAP NPs At k = 0.1546 nm, running at 40 kV and 30 mA in X-ray diffractometer (X’Pert PRO-PANalytical Philips). Zinc oxide nanoparticles were recorded in the region from 10 to 80 at a scan speed of 2h per minute. Figure 3a shows the size distribution of the Zn-TAP NPs in aqueous medium. It was measured by DLS. The average particle size was 20.23 nm. Figure 3b reveals that the zeta potential of synthesized ZnO nanoparticles was 17.6 mV. Fig. 3 a Particle size for Zn-TAP NPs from A. paniculata leaves. b Zeta potential of Zn-TAP NPs. c XRD images of Zn-TAP NPs. d SEM for Zn-TAP NPs XRD analysis of Zn-TAP NPs X-ray diffraction was used to confirm the crystalline nature of the particles by no discernible peak in the low range (2h = 1 –10 ). Figure 3c shows a representative XRD pattern of the ZnO nanoparticles synthesized by the A. paniculata extract after the complete reduction of Zn2? to Zn0. A number of Bragg reflections were present which can be indexed on the basis of the hexagonal Wurtzite structure of ZnO. The diffraction peaks at (100), (002), (101), (102) and (110) were obtained with those reported values of standard card (JCPDS no: 36–1451) [18]. )A2.5 ( ce 2 n rab1.5 sbo 1 A0.5 0 Fig. 4 a UV absorption of TAP isolated from A. paniculata leaves. b UV absorption of Zn-TAP NPs from A. paniculata leaves From the XRD peaks, to estimate the average particle size was calculated by using Scherrer’s equation as 22.23 nm. These values are merely similar to DLS size. SEM analysis of Zn-TAP NPs The synthesised Zn-TAP NPs morphology was examined by SEM in JEOL JSM-6610 LV instrument. When the nanoparticle was placed on carbon coated platinum grid, after completion of reaction, it showed a hexagonal shape as indicated in Fig. 3d. Fig. 5 a and b FTIR of TAP and Zn-TAP NPs from A. paniculata leaves UV spectroscopy studies The formation of zinc oxide nanoparticles might be due to reduction of Zn2? to Zn0 by terpenoids present in the leaf of A. paniculata. The colour transformation of A. paniculata extract treated with zinc nitrate might be due to vibrations in surface plasmon resonance of zinc [19]. Because it has free }-electrons (C=O). The broad band at 300 nm indicates the reduction of Zn2? ions which further confirmed the formation of zinc oxide nanoparticles in Fig. 4b. Whereas, the isolated active compound TAP has no absorbance change in the range of 280–350 nm (Fig. 4a). Fourier transform infrared spectroscopy (FTIR) studies The FTIR spectroscopy measurements were studied to identify the possible bio-molecules responsible for reducing the ZNO-NPs synthesised through TAP. The IR spectra of TAP (Fig. 5a) exhibit strong absorption band at 3334.71 cm-1 for O–H stretching vibration. The C–O axial stretching band appears at 1656.36 cm-1. The peak at 2852.85 cm-1 corresponds to C–H stretching vibration. Another report was assigned that the peak at 1656.36 cm-1 is due to –C=C– aromatic stretching. The peak was observed for Zn-TAP NPs between 513 and 466 cm-1 (Fig. 5b). The bands appeared between 600 and 400 cm-1 which may be assigned to the metal oxide or metal chloride [20, 21], which confirm the formation of ZnO NPs at 466.77 cm-1. The absorbance at 3479.58 cm-1 indicates the O–H stretching vibration in hydroxyl functional group of alcohols and phenol compounds of ZnO NPs. The absorption peak at 2929.87 cm-1 corresponds to –CH stretching and 2767.85 cm-1 for aldehydic –CH vibration mode. 1764.87 cm-1 is assigned Fig. 6 H1NMR for Zn-TAP NPs from A. paniculata leaves to aldehydic carbonyl (–CH=O) group. Some other bands at 1637.56, 1382.96, 1004.91 and 640.37 cm-1 were also seen which correspond to aromatic –C=C–, C–H group, – CH2 group and mono substituted ring which indicate that the TAP of A. paniculata reduced by ZnO NPs might be surrounded by aromatic ring polyphenol [22]. The band at 1637.56 cm-1 remained in ZnO NPs and this is due to groups with a benzene ring. Another report has assigned that the peak at 1637.56 cm-1 is due to –C=C– aromatic stretching [23]. In CDCl3 solvent, H1-NMR spectrum (Fig. 6) showed the 3H, s, [C=CH2 group at d 1.603. The peak at d 2.178 assigned to phenyl group stretching. The absorption peak at d 4.481 corresponds to H3C–O–CO–CH3 stretching vibration. The band appears at d 7.265 for aromatic compound. Another report was assigned that the peak at d 2.178 and d 4.481 corresponds to aliphatic –OH and aromatic –OH group respectively. TAP could be adsorbed on the surface of nanoparticles possibly by interaction through carbonyl groups or }electrons. The formation of ZnO NPs was confirmed by colour changes and was characterised by UV–Visible spectrophotometer. The broad band was observed at 300 nm. It was proved by IR spectra band at 466.77 cm-1. The presence of functional groups in ZnO NPs was also confirmed by IR and H1-NMR studies. The SEM analysis shows hexagonal shape particles with 20.23 nm size which was merely close to Scherrer’s value (i.e.) 22.23 nm. ZnTAP NPs has positive zeta potential value 17.6 mV. Hence, it was proposed that it can be easily absorbed by negatively charged cellular membrane and then contributes to efficient intracellular distribution of drug. 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S. Kavitha, M. Dhamodaran, Rajendra Prasad, M. Ganesan. Synthesis and characterisation of zinc oxide nanoparticles using terpenoid fractions of Andrographis paniculata leaves, International Nano Letters, 2017, 141-147, DOI: 10.1007/s40089-017-0207-1