Development and evaluation of an up-converting phosphor technology-based lateral flow assay for the rapid, simultaneous detection of Vibrio cholerae serogroups O1 and O139

PLOS ONE, Dec 2019

Vibrio cholerae serogroups O1 and O139 are etiological agents of cholera, a serious and acute diarrheal disease, and rapid detection of V. cholerae is a key method for preventing and controlling cholera epidemics. Here, a point of care testing (POCT) method called Vch-UPT-LF, which is an up-converting phosphor technology-based lateral flow (UPT-LF) assay with a dual-target detection mode, was developed to detect V. cholerae O1 and O139 simultaneously from one sample loading. Although applying an independent reaction pair made both detection results for the two Vch-UPT-LF detection channels more stable, the sensitivity slightly declined from 104 to 105 colony-forming units (CFU) mL−1 compared with that of the single-target assay, while the quantification ranges covering four orders of magnitude were maintained. The strip showed excellent specificity for seven Vibrio species that are highly related genetically, and nine food-borne species whose transmission routes are similar to those of V. cholerae. The legitimate arrangement of the two adjacent test lines lessened the mutual impact of the quantitation results between the two targets, and the quantification values did not differ by more than one order of magnitude when the samples contained high concentrations of both V. cholerae O1 and O139. Under pre-incubation conditions, 1×101 CFU mL−1 of V. cholerae O1 or O139 could be detected in fewer than 7 h, while the Vch-UPT-LF assay exhibited sensitivity as high as a real-time fluorescent polymerase chain reaction with fewer false-positive results. Therefore, successful development of Vch-UPT-LF as a dual-target assay for quantitative detection makes this assay a good candidate POCT method for the detection and surveillance of epidemic cholera.

A PDF file should load here. If you do not see its contents the file may be temporarily unavailable at the journal website or you do not have a PDF plug-in installed and enabled in your browser.

Alternatively, you can download the file locally and open with any standalone PDF reader:

https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0179937&type=printable

Development and evaluation of an up-converting phosphor technology-based lateral flow assay for the rapid, simultaneous detection of Vibrio cholerae serogroups O1 and O139

June Development and evaluation of an up- converting phosphor technology-based lateral flow assay for the rapid, simultaneous detection of Vibrio cholerae serogroups O1 and O139 Min Hao 0 1 2 Pingping Zhang 2 Baisheng Li 2 Xiao Liu 2 Yong Zhao 2 Hailing Tan 2 Chongyun Sun 2 Xiaochen Wang 2 Xinrui Wang 2 Haiyan Qiu 1 2 Duochun Wang 1 2 Baowei Diao 1 2 Huaiqi Jing 1 2 Ruifu Yang 2 Biao Kan 1 2 Lei Zhou 2 Ashlesh K. Murthy, Midwestern University, UNITED STATES 0 Beijing Chaoyang District Center for Disease Control and Prevention , Beijing , P. R. China , 3 Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology , Beijing , P. R. China , 4 Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329) , Beijing , P. R. China , 5 Guangdong Provincial Center for Disease Control and Prevention , Guangzhou, Guangdong , P. R. China, 6 Chongqing Entry Exit Inspection and Quarantine Bureau, Chongqing , P. R. China , 7 Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital , Beijing , P. R. China , 8 College of Animal Science and Technology, Jilin Agricultural University , Changchun, Jilin , P. R. China , 9 Institute for Plague Prevention and Control of Hebei Province , Zhangjiakou, Hebei , P. R. China , 10 Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases , Hangzhou, Zhejiang , P. R. China , 11 College of Life Sciences, Northwest University , Xi'an, Shanxi , P. R. China , 12 National Engineering Research Center for Miniaturized Detection Systems, Northwest University , Xi'an, Shanxi , P. R. China 1 State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention , Beijing , P. R. China 2 Funding: This study was supported by the National High Technology Research and Development Program of China (grant no. 2013AA032205), the Beijing Nova Program (grant no. Z151100000315086), National Key Research and Development Program (grant no. 2016YFC1202502), and Science and Technology Vibrio cholerae serogroups O1 and O139 are etiological agents of cholera, a serious and acute diarrheal disease, and rapid detection of V. cholerae is a key method for preventing and controlling cholera epidemics. Here, a point of care testing (POCT) method called VchUPT-LF, which is an up-converting phosphor technology-based lateral flow (UPT-LF) assay with a dual-target detection mode, was developed to detect V. cholerae O1 and O139 simultaneously from one sample loading. Although applying an independent reaction pair made both detection results for the two Vch-UPT-LF detection channels more stable, the sensitivity slightly declined from 104 to 105 colony-forming units (CFU) mL−1 compared with that of the single-target assay, while the quantification ranges covering four orders of magnitude were maintained. The strip showed excellent specificity for seven Vibrio species that are highly related genetically, and nine food-borne species whose transmission routes are similar to those of V. cholerae. The legitimate arrangement of the two adjacent test lines lessened the mutual impact of the quantitation results between the two targets, and the quantification values did not differ by more than one order of magnitude when the samples - Data Availability Statement: All relevant data are within the paper and its Supporting Information files. contained high concentrations of both V. cholerae O1 and O139. Under pre-incubation Program of Guangzhou, China (2014Y2-00090). The funders had no role in the study design, data collection and interpretation, or the decision to submit the work for publication. Competing interests: The authors have declared that no competing interests exist. conditions, 1×101 CFU mL−1 of V. cholerae O1 or O139 could be detected in fewer than 7 h, while the Vch-UPT-LF assay exhibited sensitivity as high as a real-time fluorescent polymerase chain reaction with fewer false-positive results. Therefore, successful development of Vch-UPT-LF as a dual-target assay for quantitative detection makes this assay a good candidate POCT method for the detection and surveillance of epidemic cholera. Introduction Cholera is a seriously infectious, acute, epidemic diarrheal disease caused by Vibrio cholerae worldwide, and especially in Asia, Africa and South America [ 1,2 ]. According to the World Health Organization, an estimated 21000 to 143000 deaths are reported for 1.3 to 4.0 million cases of cholera each year [3]. Most epidemics have been caused by V. cholerae serogroups O1 and O139 among the more than 200 V. cholerae serogroups [ 1,2 ]. V. cholerae O1 has been shown to be responsible for the sixth and seventh cholera pandemics [2]. V. cholerae O139 emerged in 1992, and it has caused epidemics in south and east Asia [ 4 ]. Plankton is the known natural reserv (...truncated)


This is a preview of a remote PDF: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0179937&type=printable

Min Hao, Pingping Zhang, Baisheng Li, Xiao Liu, Yong Zhao, Hailing Tan, Chongyun Sun, Xiaochen Wang, Xinrui Wang, Haiyan Qiu, Duochun Wang, Baowei Diao, Huaiqi Jing, Ruifu Yang, Biao Kan, Lei Zhou. Development and evaluation of an up-converting phosphor technology-based lateral flow assay for the rapid, simultaneous detection of Vibrio cholerae serogroups O1 and O139, PLOS ONE, 2017, Volume 12, Issue 6, DOI: 10.1371/journal.pone.0179937