Experimental study of ASCs combined with POC-PLA patch for the reconstruction of full-thickness chest wall defects

PLOS ONE, Dec 2019

To explore the repairing effect of combination of adipose stem cells (ASCs) and composite scaffolds on CWR, the electrospun Poly 1, 8-octanediol-co-citric acid (POC)-poly-L-lactide acid (PLA) composite scaffolds were prepared, followed by in vitro and in vivo biocompatibility evaluation of the scaffolds. Afterwards, ASCs were seeded on POC-PLA to construct the POC-PLA-ASCs scaffolds, and the POC-PLA, POC-PLA-ASCs, and traditional materials expanded polytetrafluoroethylene (ePTFE) were adopt for CWR in New Zealand white (NZW) rabbit models. As results, the POC-PLA-ASCs patches possessed good biocompatibility as the high proliferation ability of cells surrounding the patches. Rabbits in POC-PLA-ASCs groups showed better pulmonary function, less pleural adhesion, higher degradation rate and more neovascularization when compared with that in other two groups. The results of western blot indicated that POC-PLA-ASCs patches accelerated the expression of VEGF and Collagen I in rabbit models. From the above, our present study demonstrated that POC-PLA material was applied for CWR successfully, and ASCs seeded on the sheets could improve the pleural adhesions and promote the reparation of chest wall defects.

A PDF file should load here. If you do not see its contents the file may be temporarily unavailable at the journal website or you do not have a PDF plug-in installed and enabled in your browser.

Alternatively, you can download the file locally and open with any standalone PDF reader:

https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0182971&type=printable

Experimental study of ASCs combined with POC-PLA patch for the reconstruction of full-thickness chest wall defects

August Experimental study of ASCs combined with POC-PLA patch for the reconstruction of full- thickness chest wall defects Yuanzheng Zhang 0 1 Shuo Fang 0 1 Jiezhi Dai 1 Lei Zhu 1 Hao Fan 0 1 Weiya Tang 0 1 Yongjie Fan 0 1 Haiying Dai 0 1 Peipei Zhang 1 Ying Wang 1 Xin Xing 0 1 Chao Yang 0 1 0 Department of Plastic Surgery, Changhai Hospital, Second Military Medical University , Shanghai , PR China , 2 Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital , Shanghai , PR China , 3 State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University , Shanghai , PR China , 4 Department of Plastic Surgery, the 455th hospital of Chinese People's Liberation Army , Shanghai , PR China 1 Editor: Gianpaolo Papaccio, Università degli Studi della Campania "Luigi Vanvitelli" , ITALY To explore the repairing effect of combination of adipose stem cells (ASCs) and composite scaffolds on CWR, the electrospun Poly 1, 8-octanediol-co-citric acid (POC)-poly-L-lactide acid (PLA) composite scaffolds were prepared, followed by in vitro and in vivo biocompatibility evaluation of the scaffolds. Afterwards, ASCs were seeded on POC-PLA to construct the POC-PLA-ASCs scaffolds, and the POC-PLA, POC-PLA-ASCs, and traditional materials expanded polytetrafluoroethylene (ePTFE) were adopt for CWR in New Zealand white (NZW) rabbit models. As results, the POC-PLA-ASCs patches possessed good biocompatibility as the high proliferation ability of cells surrounding the patches. Rabbits in POC-PLAASCs groups showed better pulmonary function, less pleural adhesion, higher degradation rate and more neovascularization when compared with that in other two groups. The results of western blot indicated that POC-PLA-ASCs patches accelerated the expression of VEGF and Collagen I in rabbit models. From the above, our present study demonstrated that POCPLA material was applied for CWR successfully, and ASCs seeded on the sheets could improve the pleural adhesions and promote the reparation of chest wall defects. - Copyright: © 2017 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: Relevant data are within the paper and its Supporting Information files. Competing interests: The authors have declared that no competing interests exist. Introduction Intact thoracic wall plays an important role in breathing movement and protects the indispensable content. The full-thickness defects of the chest well commonly caused by tumor resection, trauma, infection, burn, and radiation [ 1,2 ]. Flaps have been developed for soft tissue reconstruction depend on the location on the chest wall, size of the defects, arc of rotation of the flap, and availability of recipient vessels. Bony reconstruction is performed with mesh or mesh combining with rib substitutes according to the number of defect ribs [3]. Optimal material for chest wall reconstruction (CWR) remains controversial. Typical mesh in clinical CWR was permanent synthetic mesh, including polypropylene and expanded polytetrafluroethylene [ 4,5 ]. While complications were associated with these material s during the post-operation follow-up, such as infection, fracture, contraction and insufficient tensile strength. Furthermore, surgical removal is inevitable when mesh infection was occurred. Bioabsorbable mesh materials, such as poly-L-lactide acid (PLA) and polydioxanone are another choice with better resistance to infection and greater implant-defect interface strength [ 6,7 ]. For rigid materials, it could afford maintenance of chest wall stability, but the lack of resilience affects the movement of the chest wall during breath, moreover the pleural adhesion between lung and mess [ 8 ]. Significant adhesions occurred in patient with thoracic surgeries account for the recurrent thoracotomies and high risk of bleeding, and the prolonged air leaks [ 9,10 ]. Poly 1, 8-octanediol-co-citric acid (POC) is reported as a biodegradable elastomer with controllable mechanical and mild inflammatory response, which has potential for use in tissue engineering [ 11,12 ]. Synthesis of POC can be conducted under very mild condition without addition of toxic catalysts, which stimulating inspiration of combination of POC with drugs or protein, such as growth factor-delivering microparticles [13]. POC-PLA composite scaffolds were widely applied in various tissue reconstruction. Electrospun composite scaffolds containing Poly (octanediol-co-citrate) are 3-dimensional elastomeric substrates potentially beneficial for CWR [ 14,15 ], while the effects of electrospun POC-PLA composite scaffolds on CWR need more explorations. Sheet-based bioengineering concept is a non-i (...truncated)


This is a preview of a remote PDF: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0182971&type=printable

Yuanzheng Zhang, Shuo Fang, Jiezhi Dai, Lei Zhu, Hao Fan, Weiya Tang, Yongjie Fan, Haiying Dai, Peipei Zhang, Ying Wang, Xin Xing, Chao Yang. Experimental study of ASCs combined with POC-PLA patch for the reconstruction of full-thickness chest wall defects, PLOS ONE, 2017, Volume 12, Issue 8, DOI: 10.1371/journal.pone.0182971