Effects of aspergillus oryzae extract (amaferm) on ruminal fibrolytic bacteria and in vitro fiber degradation

Kansas Agricultural Experiment Station Research Reports, Dec 1991

The effect of Amaferm on growth of pure cultures of ruminal cellulose-digesting, hemicellulose- digesting and pectin-digesting bacteria was determined. The addition of Amaferm to the growth medium increased the growth of Ruminococcus albus and Fibrobacter succinogenes. Amaferm had no effect on the growth of the other bacteria. Additionally, selective antimicrobial compounds were used to assess the influence of Amaferm on microbial contributions to in vitro fiber degradation. Amaferm appeared to stimulate fiber digestibility of only certain feedstuffs, and this increase in digestibility was attributed to its stimulation of bacterial activity. Amaferm did not appear to stimulate fungal activity.; Dairy Day, 1991, Kansas State University, Manhattan, KS, 1991;

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Effects of aspergillus oryzae extract (amaferm) on ruminal fibrolytic bacteria and in vitro fiber degradation

Kansas Agricultural Experiment Station Research Reports A. A. Beharka Tiruvoor G. Nagaraja Follow this and additional works at: https://newprairiepress.org/kaesrr Part of the Dairy Science Commons Recommended Citation Beharka, A. A. and Nagaraja, Tiruvoor G. (1991) "Effects of aspergillus oryzae extract (amaferm) on ruminal fibrolytic bacteria and in vitro fiber degradation," Kansas Agricultural Experiment Station Research - This report is brought to you for free and open access by New Prairie Press. It has been accepted for inclusion in Kansas Agricultural Experiment Station Research Reports by an authorized administrator of New Prairie Press. Copyright 1991 Kansas State University Agricultural Experiment Station and Cooperative Extension Service. Contents of this publication may be freely reproduced for educational purposes. All other rights reserved. Brand names appearing in this publication are for product identification purposes only. No endorsement is intended, nor is criticism implied of similar products not mentioned. K-State Research and Extension is an equal opportunity provider and employer. Effects of aspergillus oryzae extract (amaferm) on ruminal fibrolytic bacteria and in vitro fiber degradation The effect of Amaferm on growth of pure cultures of ruminal cellulose-digesting, hemicellulose- digesting and pectin-digesting bacteria was determined. The addition of Amaferm to the growth medium increased the growth of Ruminococcus albus and Fibrobacter succinogenes. Amaferm had no effect on the growth of the other bacteria. Additionally, selective antimicrobial compounds were used to assess the influence of Amaferm on microbial contributions to in vitro fiber degradation. Amaferm appeared to stimulate fiber digestibility of only certain feedstuffs, and this increase in digestibility was attributed to its stimulation of bacterial activity. Amaferm did not appear to stimulate fungal activity.; Dairy Day, 1991, Kansas State University, Manhattan, KS, 1991; Diary Day, 1991; Kansas Agricultural Experiment Station contribution; no. 92-175-S; Report of progress (Kansas Agricultural Experiment Station); 640; Dairy; Rumen; Fungal; Microbial feed additive; Growth; Fiber Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 License. This research report is available in Kansas Agricultural Experiment Station Research Reports: https://newprairiepress.org/kaesrr/vol0/iss2/43 A. A. Beharka and T. G. Nagaraja Summary The effect of Amaferm on growth of pure cultures of ruminal cellulose-digesting, hemicellulose-digesting and pectin-digesting bacteria was determined. The addition of Amaferm to the growth medium increased the growth of Ruminococcus albus and Fibrobacter succinogenes. Amaferm had no effect on the growth of the other bacteria. Additionally, selective antimicrobial compounds were used to assess the influence of Amaferm on microbial contributions to in vitro fiber degradation. Amaferm appeared to stimulate fiber digestibility of only certain feedstuffs, and this increase in digestibility was attributed to its stimulation of bacterial activity. Amaferm did not appear to stimulate fungal activity. (Key Words: Rumen, Fungal, Microbial Feed Additive, Growth, Fiber.) Introduction Reports on the use of fungal supplements in ruminant diets date back to 1924. However, results of those early studies were inconclusive. In recent years, there has been renewed interest in the use of microbial products as feed additives in ruminant diets, partly because of concerns about antibiotics. Microbial feed additives contain either the microorganisms, the dry products of microorganisms, the medium in which they grew, and/or the residues of their metabolism. The microorganisms used are yeast, molds, and/or bacteria. Because microbial products are not identical in composition, mode of action differs between products, and considerable variation in animal performance has been reported. One of several fungal products commercially available is Amaferm®, a fermentation extract of the mold Aspergillus oryzae.The addition of Amaferm or products containing Amaferm have been reported to increase digestion of dry matter, fiber, and crude protein in vivo and in vitro. In our studies with newborn calves, Amaferm supplementation was shown to increase ruminal microbial activity, as evidenced by increased VFA concentration and bacterial numbers, particularly those of fiber-digesting bacteria. The increased microbial activity was associated with increased dry feed consumption in some calves and earlier weaning. Similar increases in intake have been reported in cattle and are probably the consequence of increased rate of fiber digestion in Amaferm-supplemented animals. It has been proposed that fungal supplementation may increase the nutritive value of feedstuffs by increasing the digestion of dietary fiber. Little work has been done on the effect of Amaferm supplementation on the ruminal protoz (...truncated)


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A. A. Beharka, Tiruvoor G. Nagaraja. Effects of aspergillus oryzae extract (amaferm) on ruminal fibrolytic bacteria and in vitro fiber degradation, Kansas Agricultural Experiment Station Research Reports, 1991, pp. 1-3, Issue 2,