MT1a mRNA Expression in Human Lung Cells (BEAS 2B) After Airborne PM10 and Copper Exposure: A Possible Biomarker for Asthma

Journal of Health Disparities Research and Practice, Aug 2016

Puerto Ricans have the highest prevalence, morbidity and mortality of asthma than any other ethnic group in the U.S. and can be triggered by different stimuli such as airborne Particle pollution (PM). PM transfers transition metals, including cooper, to the human airways generating reactive oxygen species (ROS). Metallothionein protein (MT1A) is a free radical scavenger expected to be induced in lung cells by PM exposure; however, this has never been proven nor demonstrated. The hypothesis involves the amounts of PM10 in 2004, which would be significantly higher in the urban sites during months of March and June, thereby increasing PM metal exposure and inducing MT1A gene at the urban site. PM10 filters for Urban (Guaynabo) and rural (Fajardo) site were provided by the PREQB. Organic extracts were prepared from filters by means of Soxhlet Extraction and heavy metals were determined using Atomic Absorption Spectrometry. BEAS-2B cells were cultured and exposed to PM10 during 4 hrs. RNA was isolated and relative mRNA levels of MT1A were determined by RT-PCR. Urban PM10 concentrations were 35 ug/m3 and 60ug/m3 for the months of March and June, respectively. Concentrations of copper for this urban PM10 were 29.6 ppm and 50.3 ppm, for March and June, respectively. Exposure to Cu leads to an MT1A expression in lung cells demonstrating its response to metal exposure. Cellular response for the mRNA expression of MT1A was greater in relative high inflammation markers than in PM10 samples.

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MT1a mRNA Expression in Human Lung Cells (BEAS 2B) After Airborne PM10 and Copper Exposure: A Possible Biomarker for Asthma

Journal of Health Disparities Research and Practice Volume 9 MT1a mRNA Expression in Human Lung Cells (BEAS 2B) After Airborne PM10 and Copper Exposure: A Possible Biomarker for Asthma ACKNOWLEDGEMENTS 0 1 2 0 The STEP-UP HS program is supported by the National Institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health , Grant number: 1R25DK098067-01 1 Mariela Rosa-Casillas Noé Crespo-Hernández, University of Puerto Rico Mario Ortiz-Martínez, University of Puerto Rico Braulio Jiménez-Vélez, Ph.D., University of Puerto Rico, Medical Sciences Campus Coordinating Centers: University of Nevada , Las Vegas , USA 2 Journal of Health Disparities Research and Practice Volume 9, Special Edition 1, Summer 2016, pp. 135 © 2011 Center for Health Disparities Research School of Community Health Sciences University of Nevada , Las Vegas , USA Puerto Ricans have the highest prevalence, morbidity and mortality of asthma than any other ethnic group in the U.S. and can be triggered by different stimuli such as airborne Particle pollution (PM). PM transfers transition metals, including cooper, to the human airways generating reactive oxygen species (ROS). Metallothionein protein (MT1A) is a free radical scavenger expected to be induced in lung cells by PM exposure; however, this has never been proven nor demonstrated. The hypothesis involves the amounts of PM10 in 2004, which would be significantly higher in the urban sites during months of March and June, thereby increasing PM metal exposure and inducing MT1A gene at the urban site. PM10 filters for Urban (Guaynabo) and rural (Fajardo) site were provided by the PREQB. Organic extracts were prepared from filters by means of Soxhlet Extraction and heavy metals were determined using Atomic Absorption Spectrometry. BEAS-2B cells were cultured and exposed to PM10 during 4 hrs. RNA was isolated and relative mRNA levels of MT1A were determined by RT-PCR. Urban PM10 concentrations were 35 ug/m3 and 60ug/m3 for the months of March and June, respectively. Concentrations of copper for this urban PM10 were 29.6 ppm and 50.3 ppm, for March and June, respectively. Exposure to Cu leads to an MT1A expression in lung cells demonstrating its response to metal exposure. Cellular response for the mRNA expression of MT1A was greater in relative high inflammation markers than in PM10 samples. asthma; lung cells; particle matter; metallothionein; copper


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Mariela Rosa-Casillas, Noé BS Crespo-Hernández, Mario Ortiz-Martínez, Braulio Jiménez-Vélez. MT1a mRNA Expression in Human Lung Cells (BEAS 2B) After Airborne PM10 and Copper Exposure: A Possible Biomarker for Asthma, Journal of Health Disparities Research and Practice, 2016,