Ancestors’ dietary patterns and environments could drive positive selection in genes involved in micronutrient metabolism—the case of cofactor transporters
Parolo et al. Genes & Nutrition
Ancestors' dietary patterns and environments could drive positive selection in genes involved in micronutrient metabolism-the case of cofactor transporters
Silvia Parolo 0 1
Sébastien Lacroix 0 1
Marie-Pier Scott-Boyer 1
0 Equal contributors
1 The Microsoft Research, University of Trento Centre for Computational Systems Biology (COSBI) , piazza Manifattura 1, 38068 Rovereto, TN , Italy
Background: During evolution, humans colonized different ecological niches and adopted a variety of subsistence strategies that gave rise to diverse selective pressures acting across the genome. Environmentally induced selection of vitamin, mineral, or other cofactor transporters could influence micronutrient-requiring molecular reactions and contribute to inter-individual variability in response to foods and nutritional interventions. Methods: A comprehensive list of genes coding for transporters of cofactors or their precursors was built using data mining procedures from the HGDP dataset and then explored to detect evidence of positive genetic selection. This dataset was chosen since it comprises several genetically diverse worldwide populations whom ancestries have evolved in different environments and thus lived following various nutritional habits and lifestyles. Results: We identified 312 cofactor transporter (CT) genes involved in between-cell or sub-cellular compartment distribution of 28 cofactors derived from dietary intake. Twenty-four SNPs distributed across 14 CT genes separated populations into continental and intra-continental groups such as African hunter-gatherers and farmers, and between Native American sub-populations. Notably, four SNPs were located in SLC24A3 with one being a known eQTL of the NCKX3 protein. Conclusions: These findings could support the importance of considering individual's genetic makeup along with their metabolic profile when tailoring personalized dietary interventions for optimizing health.
Positive selection; Cofactor transport; Inter-individual variability; Ancestry; Dietary habits; Biological response
Diet and food availability shaped genetic variation in
humans and left distinct adaptation signals among
geographically and culturally diverse populations [
persistence in adults is the prime example of food-based
positive selection. Cattle domestication after the Neolithic
transition provided access to dairy products and the
advantages of an additional source of calories, calcium, protein,
and other nutrients [
]. The ability to utilize this nutrient
dense food resulted in a strong positive selective pressure
on a variant of the lactase-phlorizin hydrolase gene (LCT)
responsible for lactose metabolism in the small intestine
]. Other genetic changes can also be selected by food
availability. For example, the number of copies of the
salivary amylase gene may reflect adaptation to starch-rich
diets and with consequences for modern health as amylase
copy number variations may be negatively associated with
body mass index [
]. Positive adaptation signals have
also been described for FADS2, which codes for an
enzyme involved in long-chain polyunsaturated fatty acid
synthesis. A variant of FADS2 was associated with higher
mRNA expression in vegan individuals  which have
diets typically low in long chain unsaturated fatty acids.
Positive selection has also been demonstrated for genes
coding for transporters of zinc, an important cofactor of
several enzymes and DNA-binding proteins [
The objective of this study was to identify variants
showing signs of positive selection in genes coding for
cofactor transporters (hereafter referred to as CT and listed
in Additional file 1: Table S1). We posit that adaptation to
different ecological niches may also select for other genes
involved in nutrient transport and metabolism, especially
those that affect multiple cellular and biochemical
processes such as cofactors or their micronutrient precursors.
Cofactor transporter genes may be more susceptible to
being influenced by different environments and nutritional
habits because of their importance in nutrient absorption
and subsequent tissue distribution.
To fulfill this objective, genetic differentiation of
CTassociated variants were analyzed using data from the
Human Genome Diversity Project (HGDP), a dataset
chosen because it includes multiple world populations
representative of a variety of environments and ancestral
nutritional habits [
1, 13, 14
]. Using an approached based
on principal component analysis (PCA) [
variants in 14 CT genes with signals of positive selection
that could contribute to various disease risks and response
to nutritional intervention observed between individuals
with different genetic makeup were identified.
Identification of proteins involved in cofactor transport
Public databases (i.e., NCBI PubMed, UniProt, and OMIM
databases) were searched for proteins involved in the
transport of cofactors (or their nutrient precursors) between cells
or sub-cellular compartments. CTs are a subset of proteins
that transport other nutrients such as essential fatty acids
or amino acids. At least one transporter was identified for
28 of 43 nutrient-derived cofactors [
] (see the “Methods”
section for further details and Additional file 1: Table S1 for
full list of cofactors and corresponding transporters). Some
of the fat-soluble cofactors such as pyrroloquinoline
quinone (PQQ), topaquinone, qbiquinone (CoQ),
menaquinone (Vitamin K), and lipoic acid diffuse freely across
membranes and are transported in lipoproteins in the
blood. Other cofactors, such as biopterin,
tetrahydrobiopterin (BH4), molybdopterin (MPT), and
S-adenosyl-Lhomocysteine (SAH), are synthesized in cells and used
locally and as such do not require transporters. Fe-S complex,
heme-thiolate, inositol hexaphosphate, and dipyrromethane
circulate as part of hemoglobin in red blood cells. The gene
coding for the pyridoxal phosphate (vitamin B6) transporter
has not yet been identified [
A total of 312 proteins are involved in the transport of
cofactors with 39 able to transport more than one
cofactor. The transporters with affinity to the most
cofactors are the cation transporters CNNM2 (cyclin
and CBS domain divalent metal cation transport
mediator 2) and NIPAL1 (non-imprinted in Prader-Willi-like
domain containing 1) that mediate the trans-membrane
movement of five divalent cations—cobalt, copper, iron,
magnesium, and manganese.
Cofactor transporters genetic diversity
Genotype data from HGDP was used to study the genetic
differentiation in genes coding for CTs. The final sample
set included 940 individuals from 53 populations using the
quality control criteria described in the “Methods” section.
Genetic variation in CT genes was summarized by PCA.
During the computation, smartpca removed 27 subjects
belonging to Papuan and Melanesian populations because
their PC values exceeded 6 standard deviations from
population and were deemed as outliers. Nine hundred thirteen
individuals were thus included in the following analyses.
The percentage of explained variance of each PC is shown
in Additional file 2: Figure S1. First three PCs were
sufficient to separate the populations into their corresponding
continental groups using the genetic variants in CT genes.
In particular, PC1 separated African populations from all
others, PC2 described a gradient from East Asia to Middle
East and Europe, and PC3 divided Native American
populations from the others (Fig. 1 and Additional file 3: Figure S2).
The subsequent PCs described intra-continental genetic
differences. In particular, PC5 and PC6 separated the
traditional African hunter-gatherer groups (San, Mbuty Pygmy,
and Biaka Pygmy) from the African populations that adopted
the agricultural, sedentary lifestyle hereafter referred to as
farmers (Bantu from South Africa, Bantu from Kenya,
Yoruba, and Mandenka) (Additional file 4: Figure S3). The
grouping of subjects observed in the PCA of transporters
was similar to the results of PCA performed using
genome-wide genotype data (Additional file 5: Figure S4).
Positively selected SNPs and genes
A methodology based on PCA loadings was used to
identify loci under positive selection. This method does not
require a priori separation of individuals by population
and is thus beneficial with datasets such as the HGDP
composed of individuals representing a large spectrum of
genetic diversity (see the “Discussion” and “Methods”
sections for further details). This method was first tested
on the entire genome-wide dataset (Additional file 6:
Table S2). The relevance of these findings was evaluated
by further looking in the literature for the top 10 loci of
each of the first ten PCs. All these loci spanned a region
that included a SNP with a q value < 0.05, with the
exception of the SNPs related to PC1, PC2, and 1 SNP
associated to PC6 (rs11682328) that did not exceed this
threshold. Sixty-one of these 100 loci corresponded to
Ca calcium, Co cobalt, Chr chromosome, D3 vitamin D3, Fe iron, K potassium, GSH Glutathione, PC principal component, SAM S-Adenosylmethionine
aTissue enrichment category from Human Protein Atlas among the following categories: (i) Tissue enriched: mRNA levels in one tissue at least five times higher
than all other tissues, (ii) Group enriched: mRNA levels of a group of 2 to 7 tissues at least five times those of all other tissues, (iii) Tissue enhanced: mRNA levels
in a particular tissue at least five times the average level in all tissues, (iv) Expressed in all: mRNA detected in all tissues, (v) Mixed: detected in fewer than 32
tissues but not elevated in any tissue, or (vi) Not detected. Tissue(s) where protein is enriched in cases of Tissue enriched, enhanced or group enhanced is listed
Calcium voltage-gated channel
auxiliary subunit beta 4
Potassium voltage-gated channel
subfamily B member 2
Potassium voltage-gated channel
subfamily H member 5
Potassium voltage-gated channel
subfamily H member 7
Potassium two pore domain channel
subfamily K member 13
LDL receptor related protein 2
Ryanodine receptor 2
Solute carrier family 11 member 2
Solute carrier family 24 member 3
SLC25A26 Solute carrier family 25 member 26
SLCO1A2 Solute carrier organic anion transporter GSH
family member 1A2
Transient receptor potential cation
channel subfamily M member 4
Tissue enhanced: cerebral cortex; 19
Tissue enhanced: cerebral cortex 2
Tissue enriched: liver
Tissue enhanced: cerebral cortex; 8
Tissue enhanced: adrenal gland;
Tissue enriched: cerebral cortex
Tissue enhanced: testis
Group enriched: kidney; placenta; 2
Tissue enriched: heart muscle
Expressed in all
Expressed in all
Group enriched: cerebral cortex;
liver; lung; salivary gland
PC3; PC5 rs6753132 (0.05); rs6708255 (0.007);
rs16830593 (0.007); rs11902858 (0.02)
rs7833062 (0.04); rs6996335 (0.02)
rs3861656 (0.025); rs4462529 (0.025);
rs10485588 (0.04); rs16980447 (0.03);
rs6112335 (0.02); rs6035421 (0.02)
rs17044224 (0.03); rs1471476 (0.03);
genes previously described as being positively selected in
the dbPSHP database [
] (Additional file 7: Table S3)
such as, OCA2/HERC2, SLC24A5, and EDAR [
The workflow was then applied to the CT dataset.
Twenty-four SNPs corresponding to 14 CT genes
differentiated along the first five PCs (i.e., PC3 and PC5) (Table 1).
The SNPs showing evidences of positive selection in the
subsequent PCs are reported in Additional file 8: Table S4.
Positive selection in CTs was also evaluated using the
integrated Haplotype Score (iHS) selection metrics calculated
in HGDP [
] and grouping SNPs at the gene level. Most
of the genes previously identified using the PCA
workflow, with the exception of CACNA1A, HPX, SLC11A2
SLCO1A2, and TRPM4, showed evidence of positive
selection in at least one population or group of populations
using this method (detailed in the Additional file 9: Note 1).
Functional annotation and linkage disequilibrium
patterns of positively selected SNPs
SNPs showing signs of positive selection were annotated
using Ensembl transcript to investigate their functional
consequences within or flanking each gene. None were
found in exons (Additional file 10: Table S5). However,
four SNPs (rs16830593 in CACNB4, rs1471476 and
rs17044224 in SLC25A26, and rs10485588 in SLC24A3)
were identified as significant cis-eQTLs from the GTeX
eQTL database [
] (Table 2). Moreover, an additional
SNP in SLC24A3 (rs16980447) showed a nominal p value
< 0.05 but was not significant after FDR correction.
SLC24A3 SNPs were found to be associated with its
expression level in blood cells while the CACNB4 variant
was associated with its gene expression level in skin
exposed to sun. SLC25A26 SNPs were cis-eQTL in the
heart and adipose tissue. Two SNPs, rs3861656 in KCNK13
andrs16830593 in CACNB4, are likely to affect transcription
factor binding (RegulomeDB variant classification of 2b and
2c, respectively) (Additional file 11: Table S6).
Proxy SNPs using the Yoruba population from the
1000 Genomes database were used to investigate
whether non-mapped functional SNPs were in linkage
disequilibrium (LD) with SNPs differentiated in African
populations (related to PC5). No non-synonymous SNPs
were found among those in LD with the differentiated
SNPs (R-square > 0.8). However, two missense SNPs
were identified as proxy SNPs (rs6757850 correlated
with KCNH7 SNP rs6708255 and rs7588788 and
rs114005357 correlated with SLC11A2 SNP rs12312876)
when lowering the R-square threshold to 0.4. Similar
analysis was not possible for Native American
populations since no sequencing data from a different dataset
was available to evaluate LD. For what concern PC5, we
observed that the clustering of African populations in
two groups corresponded to one of the two subsistence
strategies traditionally adopted by these populations,
namely being primarily farmers or hunter-gatherers. The
best candidate gene related to PC5 is SLC24A3 since it
contains four SNPs showing evidences of positive
selection, one of which also being a strong eQTL in GTeX
database. The African genetic variation in the SLC24A3
region was further examined by estimating haplotypes to
better evaluate the difference in allele frequencies of
SLC24A3 region between the previously identified groups
of farmers and hunter-gatherers. The most common
haplotype is characterized by the SNP alleles ACAG
shared by both farmers and hunter-gatherers. Notably,
some haplotypes were restricted to only one sub-group
(Fig. 2b). Specifically, the haplotype GTAG was
separated from the network core by rs10485588 (A [red in
Fig. 3] and G [blue in Fig. 3], the ancestral and derived
alleles, respectively), the putative eQTL SNP, which is
found predominantly in farmer populations (with the
exception of two Biaka Pygmies individuals) (Fig. 3). The
haplotype with the alternative alleles for those SNPs (i.e.,
ACGA) is completely absent among farmers.
Positive selection of genes coding for proteins involved in
cofactor transport between cells or sub-cellular
compartments was found by comparing genotypes of populations
from the HGDP. This dataset is particularly interesting
Cofactors Effect size p value
SAH − 0.49 1.4E−06
From GTeX eQTL database
Ca calcium, K potassium, SAH S-Adenosyl-L-homocysteine, PC principal component
Skin—sun exposed (lower leg) Ca
since it includes genotypes from several genetically diverse
worldwide populations, whom ancestries have evolved in
different environments and thus been exposed to diets of
varying nutritional composition (i.e., hunter-gatherers and
farmers). Cofactor transporters are of particular interest as
they regulate the tissue and sub-cellular bioavailability of
micronutrient-derived cofactors and are more likely to be
influenced by different nutritional habits from ancient
populations originating from regions with varying climates
] and soil composition [
]. Cofactor-requiring biological
processes participate in normal and pathophysiological
processes that could contribute to between-population
differences in disease incidence and response to nutritional
interventions and diets [
]. However, other selective
forces may have contributed to the evolution and
distribution of CT variants among populations.
The PCA-based approach followed here associated the
population-specific alleles to a specific PC and thus a
specific ancestry gradient. Contrarily to FST statistic, a
popular measure of positive selection based on
population differentiation [
], it does not require a
priori definition of populations or groups of populations
]. We thus considered it more suitable for the HGDP
dataset, which contains several populations and some of
them not being genetically well separated from one
another. Moreover, since the PCA-based approach
identifies outlier SNPs for each principal component, it is
less likely to identify variants that underwent random
genetic drift since such phenomenon should similarly
affect all variants in a population.
The signals of positive selection identified here were
derived mainly from two PCs, namely PC3 and PC5.
The gradient described is intra-continental and is due to
the difference in allele frequencies across the Native
Americans and Africans populations, respectively. PC5
separated African hunter-gatherers from farmers, two
populations that traditionally based their subsistence on
different diets and identified SLC24A3 as being positively
selected. SLC24A3 encodes for the potassium-dependent
Na+/Ca2+ exchanger type 3 protein (NCKX3), an
important regulator of intracellular calcium homeostasis.
This gene is expressed most abundantly in the brain but
also found in the aorta, uterus, intestine, and skeletal
muscle with low expression in other tissues [
Polymorphisms in SLC24A3 have been associated with
salt-sensitive vasoconstriction and hypertension [
while the expression of NCKX3 protein was linked to
preeclampsia (i.e., pregnancy complicated by high blood
]). Selection of these variants in
huntergatherers may be due to diverse, animal-based, diets that
were low in sodium chloride and high in potassium salt
intake compared with the diet adopted after the Neolithic
]. Indeed, this transition took place at the end
of the most recent ice age and coincided with the advent of
agriculture which was characterized by increases in
plantbased at the expense of animal-based ingredients and
where salt became an important commodity. Adaptation to
such dietary pattern must have induced genetic adaptation
in many genes involved in nutrient metabolism and may
partly explain modern-day phenotypes, as that observed
recently with the FADS gene [
]. Namely, individuals
with varying admixture from hunter-gatherers to farmers,
such as modern Europeans , have different risks of
cardiovascular disease, hypertension, stroke, kidney stones,
and osteoporosis (e.g., [
]) compared to African-Americans
]), which could be mediated by their different
metabolic response to various dietary minerals. In fact, a
shortterm intervention with a hunter-gatherer, or Paleolithic, diet
improved glucose homeostasis and lipid profiles in
modernday Americans living with type II diabetes [
]. The opposite
is also possible to envision. Namely, transitioning from a
hunter-gatherer to a post-Neolithic diet could induce
metabolic alterations that, in longer-terms, would increase
cardiovascular and other chronic disease risks.
Limitations were inevitably present in the study and
should be considered when interpreting observations.
First, the HGDP dataset, obtained using the DNA chip
technology, does not allow studying rare variants that
would instead be detected using newer technology such
as the next generation sequencing. Moreover, each
population in the dataset is represented by a small
sample and could be the reason of not having extremely
significant results. In fact, even if all the SNPs reported
in the manuscript were significant after FDR correction,
only one met the genome-wide significance threshold of
p < 5 × 10−8, rs12312876 (p = 2.70 × 10−8). This issue
could be overcome using 1000 Genomes dataset;
however, the populations included in that project do not
cover the spectrum of human genetic differentiation that
would be necessary to study the selective pressure
exerted by diet. In fact, even close populations such as
the African farmers and hunter-gatherers, not present in
1000 Genomes, could have been affected by different
environmental factors. Another important limitation is
the lack of direct information on dietary habits of
reference populations that prevent any conclusion about the
driving force of the adaptation. For what concern the
analysis, the method we chose allowed us not to split
the dataset in separate populations and thus has been
the methodology of choice. However, if we had the
possibility of using larger sample sizes for the population
of interest, it would have been interesting to apply other
selection metrics such as the haplotype-based methods
such as iHS [
] and XP-EHH [
], calculated for each
population instead of groups of populations, or the
XPCLR method [
], which uses allele frequency
differentiation between populations to detect selective sweeps.
The availability of sequencing data would allow to test
also other methods, such as the population branch
statistic (PBS), which was successful in identifying genes
involved in adaptation to high altitude from exome
sequencing data [
Genetic variation in cofactor transporters may be of use
clinically to investigate and help explain inter-individual
variability in response to dietary interventions [
Indeed, individual CT SNP distribution, reflective of their
genetic backgrounds, could influence the expression or
activity of these important mediators of
micronutrientderived cofactor ADME and biological effect. Thus, our
findings support the importance of considering an
individual’s genetic makeup along with their metabolic profiles
(e.g., homeostatic measures of vitamin levels for instance)
when tailoring and analyzing responses to personalized
dietary interventions aimed at optimizing health.
Cofactor and transporter identification
NCBI PubMed (http://www.ncbi.nlm.nih.gov/pubmed),
UniProt (http://www.uniprot.org/), and OMIM (http://
www.omim.org/) databases were searched for
transporters of cofactors [
]. The cofactor name and their
synonyms with the addition of the word “transport” or
“transporter” were used for the PubMed search. For
instance, combinations of one of the following vitamin C
synonyms “vitamin C”, “vit C”, “ascorbic acid”, and
“ascorbate” AND “transporter” were searched to identify
vitamin C transporters. The transporters identified from
NCBI PubMed were verified on the UniProt database
for their involvement in the transport of other cofactors.
Tissue-specific expression of CTs was evaluated using
data extracted from the Human Protein Atlas database,
which classifies proteins into the following categories: (i)
Tissue enriched: mRNA levels in one tissue at least five
times higher than all other tissues, (ii) group enriched:
mRNA levels of a group of 2 to 7 tissues at least five
times those of all other tissues, (iii) tissue enhanced:
mRNA levels in a particular tissue at least five times the
average level in all tissues, (iv) expressed in all: mRNA
detected in all tissues, (v) mixed: detected in fewer than
32 tissues but not elevated in any tissue, or (vi) not
detected (resulting tissue-specific information can be
found in Additional file 1: Table S1) [
Genetic variation data
The genotype data were obtained from the HGDP–
CEPH panel, a resource that captures a significant
proportion of human genetic diversity. The genotypes were
obtained with the Illumina BeadStation technology for
1043 individuals, were downloaded from http://
www.hagsc.org/hgdp/files.html, and were pre-processed
at the SNP and individual levels using PLINK v1.07 [
Before the quality control procedure, 660,918 SNPs were
available. Sixteen thousand six hundred fifty
nonautosomal SNPs and 1248 SNPs with a genotyping rate
less than 0.95 and 12,085 SNPs with a minor allele
frequency less than 0.01 were excluded for a total of
630,935 remaining SNPs (of which 8960 SNPs for CT
genes). Additionally, 103 related individuals from both
first- and second-degree relative pairs, as described in
Rosenberg, 2006 [
], were also discarded. The
assignment of individuals to populations was performed using
the table downloaded from the Rosenberg Lab website
SampleInformation.txt, as published in Rosenberg, 2006
]. According to this data the HGDP individuals were
assigned to 53 populations. The geographic coordinates
were downloaded from the same web source (https://
web.stanford.edu/group/rosenberglab/data/rosenbergEtAl2005/rosenbergEtAl2005.coordinates.txt), and they
have been previously used in Rosenberg et al. [
Principal component analysis
Principal component analysis was performed with
smartpca tool of the EIGENSOFT package v6.0.1 [
using the default settings that allow the removal of
individuals detected as outliers during the computation. A
preliminary PCA on the genome-wide data was used as an
additional quality control step to detect the presence of
outliers or individuals not grouped with their geographic
region of origin, and we did not detect any issue. Next, we
used PCA to evaluate the population stratification both at
genome-wide level and on CT genes only. The pattern of
differentiation in CT genes was investigated on a subset of
8960 SNPs located in CT genes [
Statistical analyses were performed with R 3.1.2 (R
Foundation for Statistical Computing, Vienna, Austria;
http://www.r-project.org/) unless otherwise specified.
Our analysis was designed to identify SNPs with signal
of positive selection on the basis of outlier detection
from principal component analysis. Such PCA-based
approaches were recently successful in identifying genetic
loci under adaptive selection [
]. The main advantage
of this approach over other methods like FST statistic is
that it assesses genetic differentiation along gradients
without requiring a priori clustering of the individuals by
population. Starting from the SNP weights (loadings)
obtained from the smartpca output, the selection statistics
D2 was calculated and it corresponds to the squared
loading of each SNP [
]. The discrepancy between
the empirical distribution and the theoretical one was
determined, and the pchisq R function was used to associate
a p value to each SNP. p values obtained were corrected for
multiple testing using the R package q value, which controls
for false discovery rate (FDR) ). q value significance
threshold of 0.05 was used. To evaluate the results obtained
applying the selection statistic to the genome-wide HGDP
dataset, the top ten SNPs were extracted for the first ten
PCs (100 total SNPs). A genomic region spanning
200 kb around each SNP was identified and genes
annotated using the Bioconductor annotation package
TxDb.Hsapiens.UCSC.hg18.knownGene. The comparison
of results with literature was done using the data from
dbPSHP, a database which contains information about
genes and genomic regions from curated publications about
positive selection in different human populations [
Linkage disequilibrium and haplotype analysis
The identification of the proxy SNPs of each significant
variant associated to PC5 was performed using the genotype
data of 1000 genomes Yoruba population. The analysis was
carried out using the online tool LDlink
(https://analysistools.nci.nih.gov/LDlink/?tab=home). We submitted the
significant SNPs identified, and for each of them, we
retrieved a list of proxy variants located −/+ 500 Kb of the
query variant with a pairwise R2 value greater than 0.01.
The pattern of LD in SLC24A3 gene was estimated using
Haploview v4.2. The haplotype phase was inferred using
fastPHASE v1.4.8. The input files were created using
PLINK, and the tool was run using these parameters: 25
iterations of the EM algorithm (C parameter) and 200 as
the number of the number of haplotypes sampled from the
“posterior” distribution obtained from a particular random
start of the EM algorithm (H parameter). To build the
haplotype network, we used the indiv.out file which
contains estimates which attempt to minimize individual
error. The haplotype network was produced by Network
220.127.116.11 using the median-joining algorithm [
The impact of SNPs on protein function was examined
using the Ensembl Variant Effect Predictor tool (http://
www.ensembl.org/Homo_sapiens/Tools/VEP/), using the
GRCh38.p7 human assembly. The regulatory potential
of the SNPs was investigated using the RegulomeDB,
Version 1.1 [
]. The data from GTEx database V6 [
(http://www.gtexportal.org/home/) were used to
investigate the presence of correlations between the SNPs and
tissue-specific gene expression levels (i.e., eQTL).
Additional file 1: Table S1. List of all proteins identified as transporters
of cofactors. (XLSX 174 kb)
Additional file 2: Figure S1. Scree plot from PCA. This chart shows the
eigenvalues associated with each PC. (PDF 6 kb)
Additional file 3: Figure S2. PCA analysis for PC3 and PC4. The two
scatter plots show the grouping of individuals according to PC1/PC3 and
PC1/PC4. (PDF 17 kb)
Additional file 4: Figure S3. PCA analysis for PC5, 6, and 8. Principal
components showing positive selection between African sub-populations
of Hunter-gatherers and Farmers (PC5/6) and between Native Americans
(PC5/8). (PDF 19 kb)
Additional file 5: Figure S4. PCA analysis of entire HGDP dataset. The
scatter plots show the grouping of individuals according to PC1 and PC2
using all the autosomal SNPs. (PDF 10 kb)
Additional file 6: Table S2. Analysis of positive selection using the
GWAS dataset and literature comparison. (XLSX 298 kb)
Additional file 7: Table S3. SNP functional annotation. (XLSX 194 kb)
Additional file 8: Table S4. Regulome DB annotation. (XLSX 59 kb)
Additional file 9: Note 1. (DOCX 32 kb)
Additional file 10: Table S5. SNP functional annotation (XLSX 13 kb)
Additional file 11: Table S6. Regulome DB annotation. (XLSX 10 kb)
CT: Cofactor transporter; HGDP: Human genome diversity project;
IHS: Integrated haplotype score; PC: Principal components; PCA: Principal
The authors would like to acknowledge the contribution of Dr. Laura
Caberlotto in the initial data mining.
This research project was funded by the Nestlé Institute of Health Science.
Availability of data and materials
The genotypic dataset analyzed during the current study is available in the
HGDP repository, http://www.hagsc.org/hgdp/files.html. All the other data
generated or analyzed during this study are included in this published article
(and its Additional files).
MPSB and JK conceived and designed the study. SP and MPSB performed
the analyses. SL interpreted the results and drafted the manuscript. All
authors have read and approved the final manuscript.
Ethics approval and consent to participate
Consent for publication
Jim Kaput works for the Nestlé Institute of Health Sciences. The other
authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in
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1The Microsoft Research, University of Trento Centre for Computational
Systems Biology (COSBI), piazza Manifattura 1, 38068 Rovereto, TN, Italy.
2Vydiant, Inc, Gold River, CA, USA.
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