Bio-fortification potential of global wild annual lentil core collection
Bio-fortification potential of global wild annual lentil core collection
Sandeep Kumar 0 1
Anil Kumar Choudhary 1
Kuldeep Singh Rana 1
Ashutosh Sarker 1
Mohar Singh 1
0 ICAR-National Bureau of Plant Genetic Resources , Pusa, New Delhi , India , 2 Department of Agronomy, ICAR- Indian Agricultural Research Institute , New Delhi , India , 3 International Centre for Agricultural Research in Dry Areas, South Asia and China Regional Programme (SACRP), DPS Marg Pusa Campus , New Delhi , India , 4 ICAR-National Bureau of Plant Genetic Resources Regional Station , Shimla , India
1 Editor: Swarup Kumar Parida, National Institute for Plant Genome Research , INDIA
Lentil, generally known as poor man's' meat due to its high protein value is also a good source of dietary fiber, antioxidants and vitamins along with fast cooking characteristics. It could be used globally as a staple food crop to eradicate hidden hunger, if this nutritionally rich crop is further enriched with essential minerals. This requires identification of essential mineral rich germplasm. So, in the present study, a core set of 96 wild accessions extracted from 405 global wild annual collections comprising different species was analyzed to determine its bio-fortification potential. Impressive variation (mg/100 g) was observed for different minerals including Na (30±318), K (138.29±1578), P (37.50±593.75), Ca (4.74±188.75), Mg (15±159), Fe (2.82±14.12), Zn (1.29±12.62), Cu (0.5±7.12), Mn (1.22±9.99), Mo (1.02± 11.89), Ni (0.16±3.49), Pb (0.01±0.58), Cd (0±0.03), Co (0±0.63) and As (0±0.02). Hierarchical clustering revealed high intra- and inter-specific variability. Further, correlation study showed positive significant association among minerals and between minerals including agro-morphological traits. Accessions representation from Turkey and Syria had maximum variability for different minerals. Diversity analysis exhibited wide geographical variations across gene-pool in core set. Potential use of the identified trait-specific genetic resources could be initial genetic material, for genetic base broadening and biofortification of cultivated lentil.
Data Availability Statement: All relevant data are
within the paper and its Supporting Information
Funding: The authors acknowledge the help and
support provided by Department of Agriculture
Cooperation and Farmers' right, Indian Council of
Agricultural Research and International Centre for
Agricultural Research in Dry Area, India office New
Delhi. The funder had no role in study design, data
collection and analysis, decision to publish, or
preparation of the manuscript.
On earth, at least half the human population do not get sufficient daily amounts of essential
minerals, a condition leading to `hidden hunger' either due to their low concentrations in
commonly eaten staple food crops or their reduced bioavailability . Among minerals,
deficiencies specifically of iron exists within nearly 3.7 billion people worldwide comprising about
60% of the whole population [
], whereas zinc affecting about one-third of the world
], are the most common. Adequate amounts of copper, manganese, molybdenum,
cobalt, nickel, sodium and potassium are also required in body as these act as components of
various enzymes or vitamins [
] or involved in other metabolic activities [
]. Atleast one
physiological function is known for each of the nine trace elements namely iron, zinc, copper,
manganese, molybdenum, chromium, fluoride, iodine and selenium in human metabolism and
these are ranked as essential micronutrients and should be consumed regularly in food or as
]. So, there is an urgent need to identify or develop value added material for
the essential micronutrients through fortification/bio-fortification particularly in crops that
can be used as staple food, to eradicate the problem of hidden hunger.
Lentil (Lens culinaris ssp. culinaris) is the fourth most important pulse crop after bean
(Phaseolus vulgaris L.), pea (Pisum sativum L.), and chickpea (Cicer arietinum L.). It is grown widely
throughout the Indian sub-continent, the Middle East, Northern and East Africa, Southern
Europe, North and South America, Australia and western Asia [
]. The major lentil producing
countries in the world are Canada, India, Turkey, Australia, USA, Nepal, China, and Ethiopia.
The world annual production and productivity of lentil is estimated up to 4.95 million tons
and 1260 kg/ha, respectively, from cultivated area of around 4.34 million ha [
]. Lentil is
known as poor man's' meat, due to its high protein value and its fast cooking characteristics. It
is also a good source of dietary fiber, minerals and vitamins, including other functional
compounds particularly antioxidants [
]. Lentil being a nutritious crop, well adapted to dry land
agro-ecologies with poor soil fertility and crop management situations, provides an alternative
to be used as staple crop to eradicate the global hidden hunger [
Crop wild relatives (CWRs) are reservoir of important traits particularly resistance to biotic
and abiotic stresses and nutritional parameters. In our previous study, we have developed a
wild lentil core set from global collections and identified some promising accessions for yield
contributing traits including disease resistance [
], which are currently being used for genetic
base broadening of the cultivated varieties. On the other hand, legume wild species have also
been reported as a rich source of various minerals [
]. But scarce information is available on
elemental composition of wild lentil species. So in this study, wild lentil core set developed
from global collections representing wide range of genetic diversity was analyzed to determine
variations in mineral composition with an aim to find out its bio-fortification potential.
Materials and methods
A total of 96 wild lentil accessions including species L. culinaris ssp. orientalis (24 accs.), L.
culinaris ssp. odemensis (16 accs.), L. culinaris ssp. tomentosus (8 accs.), L. ervoides (24 accs.),
L. nigricans (17 accs.), L. lamottei (5 accs.) and cultivated species L. culinaris ssp. culinaris (2
accs.) were grown in plastic pots (size 24º x 24ºfilled with 10 kg of sandy loam soil in naturally
lit net house at Germplasm Evaluation Division, Indian Council of Agricultural
ResearchNational Bureau of Plant Genetic Resources (ICAR-NBPGR), New Delhi during 2013±14 and
2014±15. The details of accessions and their geographical origin are listed in Table 1. Seeds
were collected at harvest stage and analyzed for various mineral elements. All the chemicals
including standards used in the present study were of high purity.
Dried seeds of different accessions were grinded using pastle and mortar. To 500 mg of
powdered seed samples, 10 ml of concentrated nitric acid (70% HNO3) was added and kept for
overnight. Samples were then heated to 90ÊC for 16 h in digestion chamber as suggested by
Zarcinas et al. [
]. The digested samples were then filtered and made up to 100 ml with
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Sodium (Na) and potassium (K) were determined using flame photometer 128 (Systronics),
phosphorus (P) by using spectrophotometer DR5000 (HACH). Calcium (Ca) and magnesium
(Mg) were determined by using Atomic Absorption spectrophotometer (AnalytiKjena ZEEnit
700, Germany). Other mineral elements namely, iron, copper, zinc, manganese, molybdenum,
nickel, lead, cadmium, cobalt and arsenic were determined using ICP-Mass
Spectrophotometer (NexIon1 300X Series, Perkin-Elmer). All the standards of different mineral elements used
in the study were of analytical grade. All the observations were recorded in two replications.
Statistical and diversity analysis
Descriptive statistics analysis was done using Windows 2007 Microsoft Excel Data Analysis
Pack [1p5]. Phenotypic coefficient of variation (PCV) for different elements were calculated as
PCV = VP/ mean x 100as per Burton [
]. Genetic diversity among accessions based on
elemental composition data was determined from the Euclidean distances. Hierarchical
clustering and correlation analysis along with Principal Component Analysis (PCA) was carried out
using SAS software .
Mineral composition of core collection
The accessions comprising wild lentil core collection exhibited wide range of variability for
different minerals including Na (30±318 mg/100 g), K (138.29±1578 mg/100 g), P (37.50±593.75
mg/100 g), Ca (4.74±188.75 mg/100 g), Mg (15±159 mg/100 g), Fe (2.82±14.12 mg/100 g), Zn
(1.29±12.62 mg/100 g), Cu (0.5±7.12 mg/100 g), Mn (1.22±9.99 mg/100 g), Mo (1.02±11.89
mg/100 g), Ni (0.16±3.49 mg/100 g), Pb (0.01±0.58mg/100 g), Cd (0±0.03 mg/100 g), Co (0±
0.63 mg/100 g) and As (0±0.02 mg/100 g). The species-specific mineral composition of lentil
accessions is listed in Table 2. In general, the coefficient of variation (CV) was higher in
magnitude for almost all elements. The mineral data obtained in the present study was compared
with the literature values available in S1 Table. The data clearly indicated that the range of
various minerals was higher in Lens core set accessions. The essential minerals important for
biofortification purposes are further described in detail.
The maximum variability for Ca content was observed in L. nigricans with 10.47 mg/100g
(EC718266) to 188.75 mg/100 g (ILWL9) whereas, minimum variability was observed in
L. culinaris ssp. culinaris from 4.74mg/100 g (ILL8006) to 5.63 mg/100 g (ILL10829) followed
by L. culinaris ssp. odemensis with 8.48 mg/100 g (ILWL357) to 37.34 mg/100 g (ILWL35).
Accessions ILWL9 of L. nigricans from Syria and ILWL278 and ILWL343 of L. culinaris ssp.
orientalis from Turkey and Syria, had Ca content >100 mg/100 g with 188.75, 136.63, and
113.37 mg/100 g, respectively. Species L. ervoides and L. culinaris ssp. orientalis showed
substantial variability with 36.25 to 158.33 and 46 to 158 mg/100 g, respectively, for the Mg
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PLOS ONE | https://doi.org/10.1371/journal.pone.0191122
content. L. culinaris ssp. tomentosus accession ILWL90 from Turkey had highest Mg content
(159 mg/100 g). Accessions ILWL401, ILWL7, ILWL117, ILWL37, ILWL480 and ILWL66 had
Mg content of >155 mg/100 g.
In case of Fe, maximum variability of 2.82 (ILWL20) to 14.12 (EC718311) mg/100 g was
observed in L. culinaris ssp. odemensis followed by L. culinaris ssp. orientalis with 4.48
(ILWL75) to 14.12 (ILWL243) mg/100 g. However, L. culinaris ssp. tomentosus showed limited
variability for Fe content with 5.24 (EC718673) to 10.18 (ILWL305) mg/100 g. All other species
also showed reasonable variability for Fe content (Table 2). Accessions namely ILWL243
(14.12 mg/100 g), EC718311 (14.12 mg/100 g), EC718692 (13.95 mg/100 g), ILWL357 (13.62
mg/100 g), EC718275 (13.56 mg/100 g) had higher Fe content. Other accessions with higher
Fe content are listed in Table 3. In case of Zn, maximum variability was observed in L. culinaris
ssp. orientalis followed by L. culinaris ssp. odemensis as evident from the Table 2. Accessions
from L. lamottei and L. nigricans showed almost identical range for Zn with 4.12 to 12.60 and
ILWL 9 (188.75)
L. culinaris ssp. tomentosus ILWL 305 (83.97)
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4.96 to 12.62 mg/100 g, respectively. Similarly, L. culinaris ssp. tomentosus and L. ervoides also
covered the same range with 3.41 to 9.11 and 2.5 to 9.84 mg/100 g, respectively. Among the
accessions, EC718275 of L. nigricans belonging to Turkey showed highest value of 12.62 mg/
100 g followed by EC718692 of L. lamottei from France with 12.60 mg/100 g of Zn content. In
case of Cu content, maximum variability observed among accessions from L. ervoides and L.
culinaris ssp. odemensis with 1.27 (ILWL66) to 7.13 (ILWL43), and 1.18 (ILWL361) to 7.03
(ILWL56) mg/100 g, respectively. Accessions ILWL 43 (7.13 mg/ 100 g) from L. ervoides and
ILWL 56 (7.03 mg/100 g) from odemensis had higher Cu content. The species L. lamottei had a
limited range of variation from 2.31 (ILWL429) to 4.68 (ILWL29) followed by L. culinaris ssp.
culinaris with 2.46 (ILL8006) to 2.58 (ILL10829) mg/100 g. The maximum variability for Mn
content was observed among accessions from L. Culinaris ssp. orientalis with 1.22 (ILWL359)
to 9.57 (ILWL330) and L. culinaris ssp. odemensis with 1.71 (ILWL320) to 9.77 (ILWL56), mg/
100 g. However, ILWL 269 (9.99 mg/100 g) of L. ervoides from Turkey, ILWL 56 (9.77 mg/100
g) of L. culinaris ssp. odemensis from Palestinian and ILWL 330 (9.57 mg/100 g) of L. culinaris
ssp. orientalis from Syria were found with higher Mn content in comparison to other
accessions. Highest Mo content was found in L. culinaris ssp. orientalis accession ILWL476 (11.89
mg/100 g) from Turkey followed by L. culinaris ssp. odemensis accession EC718311 (11.85 mg/
100 g) from Israel.
Some accessions namely ILWL15 of L. lamottei found promising for Mg, Fe, Zn, Mn; Mo
and ILWL480 of L. culinaris ssp. odemensis for Ca, Mg, Cu, Mn, Mo; and ILWL401 of L.
ervoides for Ca, Mg, Fe and Zn. Promising accessions from different species rich in various
minerals identified are listed in Table 3.
Diversity analysis was done using data of all the 15 minerals analysed in the study. Euclidean
distances ranged from 0.845 to 14.291 as given in S2 Table. The maximum distance of 14.291
was observed between accessions ILWL7 from Turkey and ILWL227 from Syria of L. culinaris
ssp. orientalis, which was followed by L. culinaris ssp. orientalis accessions ILWL227 from
Syria and ILWL278 from Turkey with a value of 11.248, ILWL7and ILWL101, both from
Turkey with a distance value of 10.518, and ILWL278 of L. culinaris ssp. orientalis from Turkey
and ILWL16 of L. nigricans from Alpes-Cote d'Azur with 8.268. The minimum distance or
maximum similarity was observed between ILWL95 of L. culinaris ssp. orientalis and ILWL35
of L. culinaris ssp. odemensis, both from Turkey with a Euclidean distance of 0.845 followed by
L. nigricans accessions ILWL19 from Spain and EC718270 from Croatia with Euclidean
distance of 0.90, and ILWL75 of L. culinaris ssp. orientalis from Israel and ILWL198 of L. culinaris
ssp. tomentosus from Syria with a distance of 1.238. The hierarchical clustering of accessions
resulted into9different clusters A-J as shown in different colors in Fig 1. All the clusters
occupied by the accessions of different species, but in some clusters majority of the accessions were
observed species specific. Cluster C was mainly occupied by L. nigricans, E by L. culinaris ssp.
odemensis, F by L. ervoides and L. culinaris ssp. culinaris, G by L. ervoides, H by L. culinaris ssp.
orientalis. ILWL384 forms a separate cluster I. The clustering pattern indicates that grouping
of accessions was not according to their geographical origins as shown in S2 Table.
Principal Component Analysis
Principal Component Analysis was used to assess the patterns of variation by considering all
variables simultaneously. First four components have eigen value >1.0 which together
explained 65.09 per cent of total variability Table 4. PC 1 accounted for 23.2% of the total
variation, and, Mn, Cd, Cu and Mo had the highest positive coefficients means that they were the
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Fig 1. Hierarchical clustering of 96 wild lentil accessions comprising core set based on mineral composition data.
PLOS ONE | https://doi.org/10.1371/journal.pone.0191122 January 18, 2018 8 / 14
most variable traits. Similarly, PC2 explained 16.5% of the total variation, and Na and Mg had
the highest positive coefficients. PC3 accounted for 15.4% of the total variation and Fe and Zn
had the highest positive coefficients. The traits contributing to diversity are listed in Table 4.
Furthermore, the two dimensional scattering of core set accessions according to PCA and
various traits contributing to the variation are shown in Fig 2a and 2b, respectively.
Association between mineral elements and with agro-morphological traits
The correlation coefficients among the different mineral elements and with
agro-morphological traits are shown in S3 Table. Correlation analysis indicated numerous significant positive
and negative correlations. As test power increased with large number of observations, giving
Fig 2. PCA analysisÐ(2a) 2-D graph of first two principal components and (2b) various mineral elements
contributing to the variability.
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significance to most of correlations, results only with with r-values greater than 0.4 are
discussed here. Fe showed significant positive correlations with Zn (r = 0.793); Mg with Na
(0.720); Cu with Mn (r = 0.753) and Cd (r = 0.480); Mn with Ni (r = 0.436), Cd (r = 0.511), Co
(r = 0.576) and Mo (r = 0.441); Mo with Pb (r = 0.429), Cd (r = 0.615); Ni with Co (r = 0.429);
and, Pb with Cd (r = 0.525).
Elemental composition of core set accessions
The wild annual lentil core accessions used in the present study developed from the global
wild lentil germplasm accessions introduced from twenty seven counties [
]. An idea was to
study the core set accessions for detailed evaluation, which seems to be much useful from
utilization point of view. Further, it makes easy to handle reduced number of accessions for
detailed studies including biochemical, physiological and molecular analysis. Earlier also, core
and mini core collections have been evaluated for nutritional traits [
The accessions showed high variability for different minerals because core set includes
accessions from five different wild species belonging to the diverse originswith different
genetic backgrounds. Further, core set retains the genetic diversity of the whole population
from which it is developed [
]. The partitioning of mineral elements is affected by grain
morphology including grain size, embryo size, and thickness of the tissue layers. In wild and
cultivated wheat, variability in grain for Fe and Zn contents have been found related to allelic
variation at a chromosomal locus involved in remobilization of protein, Fe, Zn and Mn from
senescing leaves to seeds [
]. Recently, Gupta et al. [
] developed markers for some iron
metabolism related genes (Ferritin-1, BHLH-1, IRT-1) in lentil. Thus, the intra- as well as
interspecific variability observed for seed elemental composition in the present study also highlights
the hidden role of tight genetic control besides influence of plant morphology. Thus, the data
generated provides a valuable resource for mapping studies to identify genes or points of
regulation for elemental variation.
In general, species L. nigricans and L. culinaris ssp. orientalis showed wide variability for Ca
content, whereas L. ervoides and L. culinaris ssp. orientalis, for Mg content. Species L. culinaris
ssp. odemensis, L. culinaris ssp. orientalis and L. ervoides showed remarkable variability for
different minerals viz. Fe, Zn, Cu, Mn and Mo. This may be occurred due to diverse genetic
makeup of these species or may be due to the sound expression of respective genes of
particular species under given environmental conditions. However, L. culinaris ssp. culinaris had the
minimum variability for some of the minerals as it is a cultivated species. Singh et al. [
revealed significant effects for genotype, environment and genotype by environment
interaction for both grain Fe and Zn concentration in 50 lentil accessions.
The results revealed that in general accessions originated from Syria and Turkey had the
maximum variability for different essential minerals. The study can help us in revisiting of
Syria and Turkey regions for more useful wild annual lentil species germplasm collections
through explorations. Laghetti et al. [
] analyzed some important Italian lentil landraces for
Na (10.85±23.00 mg/100 g), P (405.4±542.4 mg/100 g), K (916.8±1071.8 mg/100 g), and Ca
(49.57±59.79 mg/ 100 g) contents. Zia-Ul Haq et al. [
] systematically analyzed four improved
lentil cultivars viz., Masoor 85, Masoor 93, NIAB Masoor 2002 and NIAB Masoor 2006 and
determined Fe (2.7±3.2mg/100 g), Cu (8.9±9.9mg/100 g), Zn (3.9±4.6mg/100 g), and Mn (1.4±
4.3mg/100 g). The micronutrient contents in Ethiopian lentil samples have been reported in
the range of 0.226 to 0.282 mg/100 g for Cu, 9.17 to 11.91 mg/100 g for Fe, 6.7 to 8.2 mg/100 g
for Mn, and 8.62 to 10.03 mg/100 g for Zn [
]. Alghamdi et al. [
] assessed mineral diversity
among 35 introduced domesticated lentil genotypes and reported pronounced variations with
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K from 674.4 to 1061.2 mg/100 g, P 286.9 to 546.7 mg/100 g, Mg 126.1 to 157.3 mg/100 g and
Ca 64.9 to 84 mg/100 g, iron 6.57 to 8.57 mg/100 g, zinc from 2.63 to 4.51 mg/100 g,
manganese 1.26 to 2.85 mg/100 g and copper 0.86 to 1.37 mg/100 g.
In the present study, Ni, Pb, Cd, Co and As were found almost within recommended range
except two-three accessions (Table 2). The data suggests that wild Lens accessions are not
good accumulators of these minerals. Leshe and Tessema [
] reported mineral composition
of Ethiopian lentil samples in the range Ni (0.120 to 0.244 mg/100 g), Pb (0.142 to 0.176 mg/
100 g), Cd (0.009 to 0.013 mg/100 g) and Co (0.285 to 0.360 mg/100 g).
The Comparison of mineral data of lentil wild species obtained in the present study with
that of the literature values of cultivated material in S1 Table highlighted that wild species
are rich source of various minerals. The variation reported from earlier studies could be due
to diverse genetic constitution, species-specificity, origin and environmental differences.
These untapped gene sources identified in the present study can be hybridized with
cultivated lentil varieties for genetic improvement of nutritional traits [
]. Earlier also, value
rich accessions have been identified for various minerals and other nutritional constituents
viz. 10 accessions each for high iron and zinc in sorghum seed, 1 accession for iron and 2
accessions for zinc in pearl millet seed, 10 accessions each for zinc, iron, protein, calcium,
and beta-carotene contents in finger millet, 5 accessions for high seed protein in chickpea,
14 accessions for zinc in pigeon pea seed [
]. Upadhyaya et al. [
] also carried out
evaluation studies of core collection of foxtail millet and obtained protein content in the range of
10.7 to 18.5%, calcium from 90.3 to 288.7 mg/kg, iron from 24.1 to 68.0 mg/kg and zinc
from 33.6 to 74.2 mg/kg.
Diversity among core set accessions
A high level of variation was obtained for various mineral nutrients as revealed by the
dendrogram. Results revealed that maximum variability among accessions was inter-specific and not
limited to a particular country. The maximum diversity was observed among the accessions
with origin from Syria and Turkey. The Euclidian distances calculated based on data from
0.845 to 14.291 showed large genetic distances among the accessions. Vieira et al. [
genetic distances up to 196.61 in 19 wheat accessions analyzed for 17 phenotypic characters. A
large genetic distance between heterotic germplasm can be useful for developing lines with
good combining ability in hybrid breeding [
]. Earlier also, based on morphological and
molecular studies, it has been established that Turkish lentil landraces had substantial genetic
diversity at the genotypic and phenotypic levels [
]. Singh et al. [
] also reported maximum
genetic diversity among wild Lens accessions with Turkey origin. Singh et al. [
] analyzed 50
lentil accessions for Fe and Zn content along with diversity analysis using 20 genomic and 54
EST-SSR markers which revealed role of geographic origin in diversity. The knowledge of the
genetic diversity among accessions provides clues about the wider genetic base of accessions/
species and that can be exploited through wide hybridization in order to develop polymorphic
populations for various traits of interest.
Principal Component Analysis
In present study of mineral composition of wild Lens accessions, PCA has been used to assess
the pattern of variations among wild lentil accessions by considering all variables
simultaneously where first four principal components accounted for 65.09% of the total variability.
Similar findings were observed by Karakoy et al. [
] where first four PCs accounted for
79.45% of the variability. They reported PC1 accounted for 36.90% of the total variation, and
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P, Zn, Mg, and K had the highest positive coefficients, whereas, PC2 explained 20.38% of
the total variation, and seed size, 100-seed weight, Mn, and Cu had the highest positive
Association studies between mineral elements and agro-morphological traits
The concentrations of several minerals, particularly Fe, Cu, Mn and Mo, were positively
correlated with other minerals suggesting that similar pathways or transporters control the uptake
and transport of these minerals. Karakoy et al. [
] reported similar observation for Zn in lentil
samples. Cabrera et al. [
] also found a direct statistical correlation between Cu±Cr, Zn±Al
and Cr±Ni and Al±Pb. The positive correlations among various minerals suggest the
possibility of breeding for increased concentrations of these elements simultaneously. The variations
in results from earlier studies can be explained on the basis that mineral composition is
influenced by a number of factors, genetic background of the materials, and environmental
Conclusions and future perspectives
High variability was recorded for various essential minerals in wild lentil core accessions
developed from global collection. Species L. culinaris ssp. odemensis, L. culinaris ssp. orientalis and
L. ervoides showed remarkable variability for different minerals viz. Fe, Zn, Cu, Mn and Mo.
In general, species, L. nigricans and L. culinaris ssp. orientalis showed wide variability for Ca
content, whereas L. ervoides and L. culinaris ssp. orientalis, for Mg content. Accessions
belonging to Turkey and Syria region showed maximum diversity for various minerals. The results
highlight the underlying tight genetic control of physiological and molecular mechanisms
involved in the mineral portioning. The genetic diversity analysis revealed intra- as well as
inter-specific variability and relationships among the wild annual Lens core set accessions.
Recently, some initiatives have been made for improvement of domesticated lentil through
bio-fortification of minerals using cross breeding approach. But efficient pre-breeding efforts
for utilizing wild Lens species for mineral bio-fortification of domesticated lentil improvement
have not been undertaken at the global level. The present study clearly indicates the potential
of wild lentil species through identification of essential mineral rich accessions including
ILWL15, ILWL480 and ILWL401 etc. which can be used for bio-fortification of cultivated
lentil using appropriate cross breeding techniques.
S1 Table. Elemental composition of core set accessions in comparison to the available literature values.
S2 Table. Clustering pattern and Euclidean distances of core set accessions based on elemental composition data.
S3 Table. Correlation among different minerals and between minerals and agro-morphological traits.
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The authors acknowledge the help and support provided by Department of Agriculture
Cooperation and Farmers' right, Indian Council of Agricultural Research and International Centre
for Agricultural Research in Dry Area, India office New Delhi.
Conceptualization: Sandeep Kumar, Mohar Singh.
Data curation: Anil Kumar Choudhary.
Project administration: Kuldeep Singh Rana, Ashutosh Sarker.
Writing ± original draft: Sandeep Kumar, Mohar Singh.
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