Preparation and characterization of Fe3O4@Au-C225 composite targeted nanoparticles for MRI of human glioma

PLOS ONE, Nov 2019

Objective To study the characterization of Fe3O4@Au-C225 composite targeted MNPs. Methods Fe3O4@Au-C225 was prepared by the absorption method. The immunosorbent assay was used to evaluate its absorption efficiency at C225 Fc. ZETA SIZER3000 laser particle size analyzer, ultraviolet photometer and its characteristics were analyzed by VSM. the targeting effect of Fe3O4@Au-C225 composite targeted MNPs on U251 cells in vitro were detected by 7.0 Tesla Micro-MR; and subcutaneous transplanted human glioma in nude mice were performed the targeting effect in vivo after tail vein injection of Fe3O4@Au-C225 composite targeted MNPs by MRI. Results The self-prepared Fe3O4@Au composite MNPs can adsorb C225 with high efficiency of adsorption so that Fe3O4@Au-C225 composite targeted MNPs were prepared successfully. Fe3O4@Au-C225 composite targeted MNPs favorably targeted human glioma cell line U251 in vitro; Fe3O4@Au-C225 composite targeted MNPs have good targeting ability to xenografted glioma on nude mice in vivo, and can be traced by MRI. Conclusion The Fe3O4@Au-C225 composite targeted MNPs have the potential to be used as a tracer for glioma in vivo.

A PDF file should load here. If you do not see its contents the file may be temporarily unavailable at the journal website or you do not have a PDF plug-in installed and enabled in your browser.

Alternatively, you can download the file locally and open with any standalone PDF reader:

https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0195703&type=printable

Preparation and characterization of Fe3O4@Au-C225 composite targeted nanoparticles for MRI of human glioma

April Preparation and characterization of Fe3O4@Au-C225 composite targeted nanoparticles for MRI of human glioma Yaoqi Ge 0 1 2 3 Yuejiao Zhong 0 1 2 Guozhong Ji 0 1 2 3 Qianling Lu 0 1 2 3 Xinyu Dai 0 1 2 3 Zhirui Guo 0 1 2 3 Peng Zhang 0 1 2 3 Gang Peng 0 1 2 3 Kangzhen Zhang 0 1 2 3 Yuntao Li 0 1 2 3 0 Natural Science Foundation of China [grant number: 81301313], the Natural Science Foundation of Jiangsu Province [grant number: BK20131015 and BK20141015], the Jiangsu Provincial College Students' Practical Innovation Training Program [grant number: 201410312015Z] , and the Nanjing Developing 1 Editor: Bing Xu, Brandeis University , UNITED STATES 2 The self-prepared Fe 3 Second Affiliated Hospital of Nanjing Medical University , Nanjing, Jiangsu Province , China , 2 Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research , Nanjing, Jiangsu Province , China 4 @Au composite MNPs can adsorb C225 with high efficiency of Conclusion for glioma in vivo. OPEN ACCESS Data Availability Statement: All relevant data are within the paper and its Supporting Information files. Objective Methods targeted MNPs by MRI. Results Project of Medical Science [grant number: YKK13174]. We would like to thank Prof. Yang Shao, Dr. Xiao Tan and Dr. Jinyan Wang for the critical reading, advice, and comments of the manuscript. Introduction The primary malignant central nervous system tumor is among 1.49% of all cancer and the rate of disability and mortality is considerably high [ 1, 2 ]. Most of these tumors originate from the gliocyte, which is commonly abbreviated to glioma. Despite advances in diagnosis and treatment for it, the prognosis is still disappointing. Therefore, there exists an urgent need for better and more effective treatment. Over the past few decades, neurologist and oncologist have dedicated to understanding the mechanisms of glioma formation and developing methods to stabilize, reduce or even eliminate the tumor. The nanometer science and technology is developing rapidly nowadays. It motivates more and more interdisciplinary integration between various subjects to create new fields of research and growing branches of the subject. The nanoscale device, with merely 1~100nm in its length, can enter and leave human cells freely. It has the advantages of less volume, better biocompatibility, and superior targeting ability to specific tissus/cells compared to traditional tracers. In our previous study, we synthesized Fe3O4@Au composite magnetic nanoparticles (MNPs) with crystal growth to optimize the preparation process and its biocompatibility [ 3 ]. Gold has a strong capacity in absorbing protein molecules, therefore provides a microenvironment which is similar to the biological molecular ontology environment. Thus, using gold as active core±shell for constructing composited MNPs helps to maintain the reactivity of biological component well [ 4 ]. Furthermore, it can enhance the stability of MNPs to improve the binding ability between MNPs and targeted molecules, resulting in stable coupling between MNPs and antibody [ 5 ]. Meanwhile, gold can mediate NIR thermotherapy as a sensitizer of light for hyperthermia depended electron-dense, dielectric property, high adsorption cross section and high Light-thermal conversion efficiency [ 6 ]. Thus, it can be used for fixing EGFR monoclonal antibody (McAb) cetuximab (C225), constructing new nanocomposite which gathers molecular targeted therapy, MRI, MFH and NIR thermotherapy by preparing Fe3O4@Au composite MNPs. The preparation and application of Fe3O4@Au composite MNPs have been reported before the present study, but the research of it used as a carrier to fix commercially available EGFR McAb-C225 for anti-glioma has not been conducted before. This article uses the method of MRI to evaluate the targeting ability of the self-prepared Fe3O4@AuC225 composite targeted MNPs in vivo and in vitro. Materials and methods Main apparatus and reagents The ultrasonic cleaner used was a CQ50 model (Ultrasonic Instrument Factory, Shanghai). The water-bathing constant temperature vibrator used was an SHZ-22 model (Medical Apparatus Factory, Taicang, Jiangsu). The automatic steam generator used was a ZFQ-B model (Xinhua Medical Apparatus Company, Shandong). The constant current electrophoresis apparatus was purchased from Liuyi Instrument Factory (Beijing). The ultraviolet detector was purchased from Tian Neng Company (Shanghai). The microplate reader used was a Multiskan MK3-353 model (USA). The double-door and dual-temperature refrigerator was purchased from Haier (China). The vacuum drying oven used was a 668 model (Dongtai Electrical Equipment Factory, Jiangsu). The vibrating sample magnetometer (VSM) used was a PPMS-9 model (Quantum Design, USA). A 752 prismatic ultraviolet-visible (UV-vis) spectrophotometer was purchased from Shanghai Precision Scientific Instrument Co, Ltd, (Shanghai, China). The 7.0Tesla Micro- MR was purchased from PharmaScan (Br (...truncated)


This is a preview of a remote PDF: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0195703&type=printable

Yaoqi Ge, Yuejiao Zhong, Guozhong Ji, Qianling Lu, Xinyu Dai, Zhirui Guo, Peng Zhang, Gang Peng, Kangzhen Zhang, Yuntao Li. Preparation and characterization of Fe3O4@Au-C225 composite targeted nanoparticles for MRI of human glioma, PLOS ONE, 2018, Volume 13, Issue 4, DOI: 10.1371/journal.pone.0195703