Preparation and characterization of Fe3O4@Au-C225 composite targeted nanoparticles for MRI of human glioma
April
Preparation and characterization of Fe3O4@Au-C225 composite targeted nanoparticles for MRI of human glioma
Yaoqi Ge 0 1 2 3
Yuejiao Zhong 0 1 2
Guozhong Ji 0 1 2 3
Qianling Lu 0 1 2 3
Xinyu Dai 0 1 2 3
Zhirui Guo 0 1 2 3
Peng Zhang 0 1 2 3
Gang Peng 0 1 2 3
Kangzhen Zhang 0 1 2 3
Yuntao Li 0 1 2 3
0 Natural Science Foundation of China [grant number: 81301313], the Natural Science Foundation of Jiangsu Province [grant number: BK20131015 and BK20141015], the Jiangsu Provincial College Students' Practical Innovation Training Program [grant number: 201410312015Z] , and the Nanjing Developing
1 Editor: Bing Xu, Brandeis University , UNITED STATES
2 The self-prepared Fe
3 Second Affiliated Hospital of Nanjing Medical University , Nanjing, Jiangsu Province , China , 2 Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research , Nanjing, Jiangsu Province , China
4 @Au composite MNPs can adsorb C225 with high efficiency of
Conclusion
for glioma in vivo.
OPEN ACCESS
Data Availability Statement: All relevant data are
within the paper and its Supporting Information
files.
Objective
Methods
targeted MNPs by MRI.
Results
Project of Medical Science [grant number:
YKK13174]. We would like to thank Prof. Yang
Shao, Dr. Xiao Tan and Dr. Jinyan Wang for the
critical reading, advice, and comments of the
manuscript.
Introduction
The primary malignant central nervous system tumor is among 1.49% of all cancer and the
rate of disability and mortality is considerably high [
1, 2
]. Most of these tumors originate from
the gliocyte, which is commonly abbreviated to glioma. Despite advances in diagnosis and
treatment for it, the prognosis is still disappointing. Therefore, there exists an urgent need for
better and more effective treatment. Over the past few decades, neurologist and oncologist
have dedicated to understanding the mechanisms of glioma formation and developing
methods to stabilize, reduce or even eliminate the tumor.
The nanometer science and technology is developing rapidly nowadays. It motivates more
and more interdisciplinary integration between various subjects to create new fields of
research and growing branches of the subject. The nanoscale device, with merely 1~100nm in
its length, can enter and leave human cells freely. It has the advantages of less volume, better
biocompatibility, and superior targeting ability to specific tissus/cells compared to traditional
tracers. In our previous study, we synthesized Fe3O4@Au composite magnetic nanoparticles
(MNPs) with crystal growth to optimize the preparation process and its biocompatibility [
3
].
Gold has a strong capacity in absorbing protein molecules, therefore provides a
microenvironment which is similar to the biological molecular ontology environment. Thus, using gold as
active core±shell for constructing composited MNPs helps to maintain the reactivity of
biological component well [
4
]. Furthermore, it can enhance the stability of MNPs to improve the
binding ability between MNPs and targeted molecules, resulting in stable coupling between
MNPs and antibody [
5
]. Meanwhile, gold can mediate NIR thermotherapy as a sensitizer of
light for hyperthermia depended electron-dense, dielectric property, high adsorption cross
section and high Light-thermal conversion efficiency [
6
]. Thus, it can be used for fixing EGFR
monoclonal antibody (McAb) cetuximab (C225), constructing new nanocomposite which
gathers molecular targeted therapy, MRI, MFH and NIR thermotherapy by preparing
Fe3O4@Au composite MNPs. The preparation and application of Fe3O4@Au composite MNPs
have been reported before the present study, but the research of it used as a carrier to fix
commercially available EGFR McAb-C225 for anti-glioma has not been conducted before. This
article uses the method of MRI to evaluate the targeting ability of the self-prepared
Fe3O4@AuC225 composite targeted MNPs in vivo and in vitro.
Materials and methods
Main apparatus and reagents
The ultrasonic cleaner used was a CQ50 model (Ultrasonic Instrument Factory, Shanghai).
The water-bathing constant temperature vibrator used was an SHZ-22 model (Medical
Apparatus Factory, Taicang, Jiangsu). The automatic steam generator used was a ZFQ-B model
(Xinhua Medical Apparatus Company, Shandong). The constant current electrophoresis
apparatus was purchased from Liuyi Instrument Factory (Beijing). The ultraviolet detector was
purchased from Tian Neng Company (Shanghai). The microplate reader used was a Multiskan
MK3-353 model (USA). The double-door and dual-temperature refrigerator was purchased
from Haier (China). The vacuum drying oven used was a 668 model (Dongtai Electrical
Equipment Factory, Jiangsu). The vibrating sample magnetometer (VSM) used was a PPMS-9
model (Quantum Design, USA). A 752 prismatic ultraviolet-visible (UV-vis)
spectrophotometer was purchased from Shanghai Precision Scientific Instrument Co, Ltd, (Shanghai, China).
The 7.0Tesla Micro- MR was purchased from PharmaScan (Br (...truncated)