Advanced search    

Search: authors:"Hui Liu"

329 papers found.
Use AND, OR, NOT, +word, -word, "long phrase", (parentheses) to fine-tune your search.

Direct reprogramming of porcine fibroblasts to neural progenitor cells

, Ting-Ting Yuan, Guo-Hong Yuan, Li-Na Sui, Di Guan, Shun-Lei Duan, Hui-Ze Pan, Ping Wang, Xi-Ping Zhu, Nuria Montserrat, Ming Li, Rui-Jun Bai, Lin Liu, Juan Carlos Izpisua Belmonte, and Guang-Hui Liu

SEC24A deficiency lowers plasma cholesterol through reduced PCSK9 secretion

-Hui Liu 4 7 Elizabeth Adams 4 6 Andrea Baines 4 6 Genggeng Yu 3 4 Maureen A Sartor 4 8 Bin Zhang 4 7 Zhengping Yi 4 5 Jiandie Lin 3 4 Stephen G Young 4 10 Randy Schekman 4 9 David Ginsburg 2 4 0

Evaluation of the Value of Fasting Plasma Glucose in the First Prenatal Visit to Diagnose Gestational Diabetes Mellitus in China

WEI-WEI ZHU HUI-XIA YANG YU-MEI WEI JIE YAN PHD ZI-LIAN WANG XUE-LAN LI MM HAI-RONG WU MM NAN LI MM MEI-HUA ZHANG MM XING-HUI LIU MM HUA ZHANG YUN-HUI WANG MM JIAN-MIN NIU MM YU-JIE GAN MM LI-RUO

Induction of Oxidative Stress Response by the Mycotoxin Patulin in Mammalian Cells

Patulin (PAT), a mycotoxin mainly produced by Penicillium and Aspergillus, is found in various foods and feeds. In the present study, its effects on oxidative stress in various mammalian cell lines were investigated. When cell-permeating fluorescent dyes were used as indicators of the generation of reactive oxygen species (ROS), we found that PAT treatment directly increased...

RNA and DNA, which contain two GGAGG segments connected with UUUU or TTTT sequences, form entirely different quadruplex structures

We determined solution structure of d(GGAGGTTTTGGAGG)(D14) in the presence of Na+ ions by NMR. Two molecules of D14 form a quadruplex strucure with parallel and antiparallel strand alignments. The quadruplex is formed by four helical GGAGG segments and two diagonal TTTT loops at the top and bottom of the helix. This quadruplex structure is entirely different from that of r...

Diel vertical migration of zooplankton following optimal food intake under predation

A model is developed to investigate the trade-offs between benefits and costs involved in zooplanktonic diel vertical migration (DVM) strategies. The ‘venturous revenue’ (VR) is used as the criterion for optimal trade-offs. It is a function of environmental factors and the age of zooplankter. During vertical migration, animals are assumed to check instantaneously the variations...

GeneLibrarian: an effective gene-information summarization and visualization system

Background Abundant information about gene products is stored in online searchable databases such as annotation or literature. To efficiently obtain and digest such information, there is a pressing need for automated information-summarization and functional-similarity clustering of genes. Results We have developed a novel method for semantic measurement of annotation and...

Microarray Comparative Genomic Hybridisation Analysis Incorporating Genomic Organisation, and Application to Enterobacterial Plant Pathogens

Microarray comparative genomic hybridisation (aCGH) provides an estimate of the relative abundance of genomic DNA (gDNA) taken from comparator and reference organisms by hybridisation to a microarray containing probes that represent sequences from the reference organism. The experimental method is used in a number of biological applications, including the detection of human...

Nitric Oxide Destabilizes Pias3 and Regulates Sumoylation

Small ubiquitin-related protein modifiers (SUMO) modification is an important mechanism for posttranslational regulation of protein function. However, it is largely unknown how the sumoylation pathway is regulated. Here, we report that nitric oxide (NO) causes global hyposumoylation in mammalian cells. Both SUMO E2 conjugating enzyme Ubc9 and E3 ligase protein inhibitor of...

Mycotoxin Patulin Activates the p38 Kinase and JNK Signaling Pathways in Human Embryonic Kidney Cells

Patulin (PAT), a mycotoxin mainly produced by Penicillium and Aspergillus, is frequently detected in moldy fruits and fruit products. Exposure of human embryonic kidney (HEK293) cells to PAT led to a dose- and time-dependent increase in the phosphorylation of two major mitogen-activated protein kinases (MAPKs), p38 kinase and c-Jun N-terminal kinase (JNK). The phosphorylated...

Quadruplex structures of RNA 14-mer, r(GGAGGUUUUGGAGG) and DNA 14-mer, d(GGAGGTTTTGGAGG)

We have determined solution structure of r(GGAGGUUUUGGAGG) (R14) by NMR; the RNA 14-mer forms an intra-strand parallel quadruplex with a G-tetrad and a hexad, in which a G-tetrad core is augmented by association of two A residues. The quadruplex further forms a dimer through stacking interaction between the hexads. We also synthesized the corresponding DNA 14-mer, d...

Developmental incompetency of denuded mouse oocytes undergoing maturation in vitro is ooplasmic in nature and is associated with aberrant Oct-4 expression

BACKGROUND: Germinal vesicle (GV) oocytes constitute a potential resource but their developmental competence is questionable especially when surrounding cumulus cells are removed. The intercellular factors/mechanisms underlying such poor embryonic competence may originate at a nuclear and/or ooplasmic level. METHODS: Immature or mature oocytes were obtained from three mouse...

In-vitro development of mouse zygotes following reconstruction by sequential transfer of germinal vesicles and haploid pronuclei

We evaluated whether mouse oocytes reconstructed by germinal vesicle (GV) transfer can develop to blastocyst stage. The oocytes were artificially activated with sequential treatment of A23187 and anisomycin; fertilization was then established by transfer or exchange of pronuclei with those of zygotes fertilized in vivo. Type 1 zygotes were constructed by placing the male haploid...

Systemic treatment of xenografts with vaccinia virus GLV-1h68 reveals the immunologic facet of oncolytic therapy

Background GLV-1h68 is an attenuated recombinant vaccinia virus (VACV) that selectively colonizes established human xenografts inducing their complete regression. Results Here, we explored xenograft/VACV/host interactions in vivo adopting organism-specific expression arrays and tumor cell/VACV in vitro comparing VACV replication patterns. There were no clear-cut differences in...

Metaphase II nuclei generated by germinal vesicle transfer in mouse oocytes support embryonic development to term

BACKGROUND: Cytoplasmic defects are thought to cause aneuploidies in oocytes and embryos and oocyte ‘reconstruction’ by germinal vesicle (GV) transfer may circumvent such defects. In mice ‘reconstructed’ oocytes undergo meiosis and fertilize normally, but early embryonic development is compromised if their ooplasm matured in vitro. This study employs sequential MII spindle and/or...

The Identification of Lymphocyte-Like Cells and Lymphoid-Related Genes in Amphioxus Indicates the Twilight for the Emergency of Adaptive Immune System

To seek evidence of a primitive adaptive immune system (AIS) before vertebrate, we examined whether lymphocytes or lymphocyte-like cells and the related molecules participating in the lymphocyte function existed in amphioxus. Anatomical analysis by electron microscopy revealed the presence of lymphocyte-like cells in gills, and these cells underwent morphological changes in...

Quorum Sensing Coordinates Brute Force and Stealth Modes of Infection in the Plant Pathogen Pectobacterium atrosepticum

Quorum sensing (QS) in vitro controls production of plant cell wall degrading enzymes (PCWDEs) and other virulence factors in the soft rotting enterobacterial plant pathogen Pectobacterium atrosepticum (Pba). Here, we demonstrate the genome-wide regulatory role of QS in vivo during the Pba–potato interaction, using a Pba-specific microarray. We show that 26% of the Pba genome...

New quadruplex structure of GGA triplet repeat DNA—An intramolecular quadruplex composed of a G:G:G:G tetrad and G(:A):G(:A):G(:A):G heptad, and its dimerization—

The structure of d(GGAGGAGGAGGA) containing four tandem repeats of a GGA triplet sequence has been determined under physiological K+ conditions by NMR. d(GGAGGAGGAGGA) folds into an intramolecular quadruplex composed of a G:G:G:G tetrad and a G(:A):G(:A):G(:A):G heptad. Four G-G segments of d(GGAGGAGGAGGA) are aligned parallel to each other due to seven successive turns of the...

Orexin A Preferentially Excites Glucose-Sensitive Neurons in the Lateral Hypothalamus of the Rat In Vitro

Falls in blood glucose induce hunger and initiate feeding. The lateral hypothalamic area (LHA) contains glucose-sensitive neurons (GSNs) and orexin neurons, both of which are stimulated by falling blood glucose and are implicated in hypoglycemia-induced feeding. We combined intracellular electrophysiological recording with fluorescein labeling of GSNs to determine their...