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139 papers found.
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Prediction of cancer progression in a group of 73 gastric cancer patients by circulating cell-free DNA

Background Circulating cell-free DNA (ccf-DNA) in plasma may contain both specific and non-specific of tumor markers. The concentration and integrity of ccf-DNA may be clinical useful for detecting and predicting cancer progression. Methods Plasma samples from 40 healthy controls and 73 patients with gastric cancers (two stage 0, 17 stage I, 11 stage II, 33 stage III, and 10...

A single mutation in the PBC loop of VP2 is involved in the in vitro replication of infectious bursal disease virus

To test whether amino acid mutations in the PBC and PHI loops of VP2 are involved in the replication and virulence of infectious bursal disease virus (IBDV), a pair of viruses, namely the moderately virulent IBDV (rGx-F9VP2) and the attenuated strain (rGt), were used. Residue mutations A222P (PBC) and S330R (PHI), selected by sequence comparison, were introduced individually into...

Glutamine Reduces the Apoptosis of H9C2 Cells Treated with High-Glucose and Reperfusion through an Oxidation-Related Mechanism

Mitochondrial overproduction of reactive oxygen species (ROS) in diabetic hearts during ischemia/reperfusion injury and the anti-oxidative role of glutamine have been demonstrated. However, in diabetes mellitus the role of glutamine in cardiomyocytes during ischemia/reperfusion injury has not been explored. To examine the effects of glutamine and potential mechanisms, in the...

Safety and Efficacy of Thermal Ablation for Small Renal Masses in Solitary Kidney: Evidence from Meta-Analysis of Comparative Studies

Objective To evaluate comparative renal functional preservation, perioperative and oncologic outcomes, and complications of thermal ablation (TA) versus partial nephrectomy (PN) in management of Small renal masses (SRMs) in solitary kidney. Methods and Findings Medline, Embase, Web of Science and the Cochrane Library were systematically searched. A meta-analysis for comparative...

Identification and Characterization of a Novel Monoterpene Synthase from Soybean Restricted to Neryl Diphosphate Precursor

Terpenes are important defensive compounds against herbivores and pathogens. Here, we report the identification of a new monoterpene synthase gene, GmNES, from soybean. The transcription of GmNES was up-regulated in soybean plants that were infested with cotton leafworm (Prodenia litura), mechanically wounded or treated with salicylic acid (SA). Gas chromatography-mass...

Prostatic arterial embolization for the treatment of lower urinary tract symptoms due to large (>80 mL) benign prostatic hyperplasia: results of midterm follow-up from Chinese population

population Mao Qiang Wang 0 Li Ping Guo 0 Guo Dong Zhang Kai Yuan Kai Li Feng Duan 0 Jie Yu Yan Yan Wang Hai Yan Kang Zhi Jun Wang 0 Equal contributors Department of Interventional Radiology, Chinese PLA

Optimization of Genome Engineering Approaches with the CRISPR/Cas9 System

Designer nucleases such as TALENS and Cas9 have opened new opportunities to scarlessly edit the mammalian genome. Here we explored several parameters that influence Cas9-mediated scarless genome editing efficiency in murine embryonic stem cells. Optimization of transfection conditions and enriching for transfected cells are critical for efficiently recovering modified clones...

An aberrant spliced transcript of focal adhesion kinase is exclusively expressed in human breast cancer

Purpose To clarify the roles of a new aberrantly spliced transcript of FAK that lacks exon 26 (denoted -26-exon FAK) in human breast cancers. Methods Transcripts of FAK expressed in 102 human breast tumor tissues and 52 corresponding normal tissues were analyzed by RT-PCR and DNA sequencing, as well as agarose gel electrophoresis. The cDNA of -26-exon FAK was cloned and expressed...

Water Use Patterns of Sympatric Przewalski’s Horse and Khulan: Interspecific Comparison Reveals Niche Differences

Acquiring water is essential for all animals, but doing so is most challenging for desert-living animals. Recently Przewalski’s horse has been reintroduced to the desert area in China where the last wild surviving member of the species was seen before it vanished from China in the1960s. Its reintroduction placed it within the range of a close evolutionary relative, the con...

Down-Regulation of Integrin β1 and Focal Adhesion Kinase in Renal Glomeruli under Various Hemodynamic Conditions

Given that integrin β1 is an important component of the connection to maintain glomerular structural integrity, by binding with multiple extracellular matrix proteins and mediating intracellular signaling. Focal adhesion kinase (FAK) is the most essential intracellular integrator in the integrin β1-FAK signalling pathway. Here, we investigated the changes of the two molecules and...

Using Gelatin Nanoparticle Mediated Intranasal Delivery of Neuropeptide Substance P to Enhance Neuro-Recovery in Hemiparkinsonian Rats

Purpose Intranasal administration of phospholipid-based gelatin nanoparticles (GNP) was prepared to investigate the neuro-recovery effects of neuropeptide Substance P (SP) on hemiparkinsonian rats. Methods The SP-loaded gelatin nanoparticles (SP-GNP) were prepared by a water-in-water emulsion method and possessed high stability, encapsulating efficiency and loading capacity. PC...

Purinergic control of AMPK activation by ATP released through connexin 43 hemichannels – pivotal roles in hemichannel-mediated cell injury

Connexin hemichannels regulate many cell functions. However, the molecular mechanisms involved remain elusive. Hemichannel opening causes loss of ATP, we therefore speculated a potential role for AMPK in the biological actions of hemichannels. Activation of hemichannels by removal of extracellular Ca2+ led to an efflux of ATP and a weak activation of AMPK. Unexpectedly...

Early versus delayed laparoscopic common bile duct exploration for common bile duct stone-related nonsevere acute cholangitis

, Yan Wang, Kai Li, Buhe Amin, Ke Gong, Yiping Lu & Nengwei ZhangDepartment of medicine, Division of Gastroenterology, Hepatology and Nutrition, University of Louisville School of Medicine, Louisville ... Research journals • PubMed • Google ScholarSearch for Ying Li in:Nature Research journals • PubMed • Google ScholarSearch for Yan Wang in:Nature Research journals • PubMed • Google ScholarSearch for Kai Li

Targeted Overexpression of α-Synuclein by rAAV2/1 Vectors Induces Progressive Nigrostriatal Degeneration and Increases Vulnerability to MPTP in Mouse

Mutations, duplication and triplication of α-synuclein genes are linked to familial Parkinson’s disease (PD), and aggregation of α-synuclein (α-syn) in Lewy bodies (LB) is involved in the pathogenesis of the disease. The targeted overexpression of α-syn in the substantia nigra (SN) mediated by viral vectors may provide a better alternative to recapitulate the neurodegenerative...

Transcriptomic analysis of differentially expressed genes in the Ras1 CA-overexpressed and wildtype posterior silk glands

Background Using the piggyBac-mediated GAL4/UAS transgenic system established in the silkworm, Bombyx mori, we have previously reported that overexpression of the Ras1 CA oncogene specifically in the posterior silk gland (PSG) improved cell growth, fibroin synthesis, and thus silk yield. However, the detailed molecular mechanism remains to be fully elucidated. To achieve this...

A novel model for evaluating thrombolytic therapy in dogs with ST-elevation myocardial infarction

Background There is still no standard large animal model for evaluating the effectiveness of potential thrombolytic therapies. Here, we aimed to develop a new beagle model with ST-elevation myocardial infarction (STEMI) by injecting autologous emboli with similar components of coronary thrombus. Methods 18 male beagles were included and divided into three groups: red embolus...

A Miniaturize Bandpass Filter with Harmonic Suppression Using Meandered Quarter-Wavelength Resonators

significant suggestion to revise the paper; Tingting Gu and Ping Cao assisted the authors to simulate and optimize the proposed filter; Kai Li discussed the design and helped the authors complete the original

Signaling pathways in the development of infantile hemangioma

Infantile hemangioma (IH), which is the most common tumor in infants, is a benign vascular neoplasm resulting from the abnormal proliferation of endothelial cells and pericytes. For nearly a century, researchers have noted that IH exhibits diverse and often dramatic clinical behaviors. On the one hand, most lesions pose no threat or potential for complication and resolve...

Contribution of NKX2-3 Polymorphisms to Inflammatory Bowel Diseases: A Meta-Analysis of 35358 subjects

Guangzhou Road, Nanjing, Jiangsu, 210029, ChinaXiaoCheng Lu, Linjun Tang, Kai Li, JinYu Zheng, Penglai Zhao, Yi Tao & Li-Xin Li AuthorsSearch for XiaoCheng Lu in:Nature Research journals • PubMed • Google ... ScholarSearch for Linjun Tang in:Nature Research journals • PubMed • Google ScholarSearch for Kai Li in:Nature Research journals • PubMed • Google ScholarSearch for JinYu Zheng in:Nature Research journals

Genome-Wide Analysis of Long Noncoding RNA (lncRNA) Expression in Hepatoblastoma Tissues

Long noncoding RNAs (lncRNAs) have crucial roles in cancer biology. We performed a genome-wide analysis of lncRNA expression in hepatoblastoma tissues to identify novel targets for further study of hepatoblastoma. Hepatoblastoma and normal liver tissue samples were obtained from hepatoblastoma patients. The genome-wide analysis of lncRNA expression in these tissues was performed...