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Identification of QTLs associated with tissue culture response of mature wheat embryos

Mature embryo is an excellent explant for tissue culture as it is convenient to be obtained without limitation of growing seasons and development stages. However, regeneration ability of the calli from wheat mature embryos is limited, thus hindering its application. To identify genes associated with the tissue culture response (TCR) of wheat, QTLs for callus induction from mature...

Integrated analysis of genome-wide DNA methylation, gene expression and protein expression profiles in molecular subtypes of WHO II-IV gliomas

Background Glioma is the most common malignant primary brain tumor among adults, among which glioblastoma (GBM) exhibits the highest malignancy. Despite current standard chemoradiation, glioma is still invariably fatal. A further insight into the molecular background of glioma is required to improve patient outcomes. Method Previous studies evaluated molecular genetic differences...

Phylogeny-based classification of microbial communities

Motivation: Next-generation sequencing coupled with metagenomics has led to the rapid growth of sequence databases and enabled a new branch of microbiology called comparative metagenomics. Comparative metagenomic analysis studies compositional patterns within and between different environments providing a deep insight into the structure and function of complex microbial...

bmo-miR-0001 and bmo-miR-0015 down-regulate expression of Bombyx mori fibroin light chain gene in vitro

mechanisms of silk protein biosynthesis. Compliance with ethics guidelines Chen CHEN, Yang-yang FAN, Xin WANG, Fei SONG, Tao JIANG, Ping QIAN, Shun-ming TANG, and Xing-jia SHEN declare that they have no

Release of Si from Silicon, a Ferrosilicon (FeSi) Alloy and a Synthetic Silicate Mineral in Simulated Biological Media

Unique quantitative bioaccessibility data has been generated, and the influence of surface/material and test media characteristics on the elemental release process were assessed for silicon containing materials in specific synthetic body fluids at certain time periods at a fixed loading. The metal release test protocol, elaborated by the KTH team, has previously been used for...

Accurate inference of isoforms from multiple sample RNA-Seq data

Background RNA-Seq based transcriptome assembly has become a fundamental technique for studying expressed mRNAs (i.e., transcripts or isoforms) in a cell using high-throughput sequencing technologies, and is serving as a basis to analyze the structural and quantitative differences of expressed isoforms between samples. However, the current transcriptome assembly algorithms are...

Adiponectin improves NF-κB-mediated inflammation and abates atherosclerosis progression in apolipoprotein E-deficient mice

Background Atherosclerosis is a common pathological basis of cardiovascular disease. Adiponectin (APN) has been shown to have an anti-atherosclerosis effect, and the underlying mechanisms, however, are largely unknown. Nuclear factor κB (NF-κB) has also been regarded as a proatherogenic factor, mainly because of its regulation of a variety of the proinflammatory genes linked to...

Development of an immunochromatographic strip for the serodiagnosis of Theileria infection in sheep

Background Theileria uilenbergi and T. luwenshuni are tick-borne protozoan parasites, transmitted by Haemaphysalis qinghaiensis and H. longicornis, respectively. They are the main causative agents of theileriosis in small ruminants in China. The disease has resulted in severe economic losses and hindered the development of sheep and goat husbandry industry in the endemic regions...

Diagnostic Value of SFRP1 as a Favorable Predictive and Prognostic Biomarker in Patients with Prostate Cancer

Growing genetic and molecular biological evidence suggests that the disruption of balance between Secreted Frizzled-Related Protein-1 (SFRP1) and β-catenin plays an important role in the initiation and development of multiple cancers. The aim of this study was to examine whether the expression of SFRP1 and β-catenin is associated with the clinical-pathologic features of patients...

The Tissue Response and Degradation of Electrospun Poly(ε-caprolactone)/Poly(trimethylene-carbonate) Scaffold in Subcutaneous Space of Mice

Injury, Tianjin 300162, China Received 15 May 2014; Revised 8 August 2014; Accepted 16 August 2014; Published 3 September 2014 Academic Editor: Xingcai Wu Copyright © 2014 Tao Jiang et al. This is an

Tumor Detection at 3 Tesla with an Activatable Cell Penetrating Peptide Dendrimer (ACPPD-Gd), a T1 Magnetic Resonance (MR) Molecular Imaging Agent

Purpose The ability to detect small malignant lesions with magnetic resonance imaging (MRI) is limited by inadequate accumulations of Gd with standard chelate agents. To date, no T1-targeted agents have proven superiority to Gd chelates in their ability to detect small tumors at clinically relevant field strengths. Activatable cell-penetrating peptides and their Gd-loaded...

Comparison of Retrograde Balloon Dilatation and Laparoscopic Pyeloplasty for Treatment of Ureteropelvic Junction Obstruction: Results of a 2-Year Follow-Up

Objective To evaluate the efficacy of laparoscopic pyeloplasty relative to retrograde balloon dilatation for the treatment of ureteropelvic junction obstruction (UPJO). Methods This retrospective study enrolled UPJO patients with stricture length < 2 cm who had been treated with laparoscopic pyeloplasty (LP; 44 cases) or balloon dilatation (BD; 38 cases) from Jan 2010 to Jan 2012...

TIMP1 is a prognostic marker for the progression and metastasis of colon cancer through FAK-PI3K/AKT and MAPK pathway

Background Tissue inhibitor matrix metalloproteinase 1 (TIMP1) plays a vital role in carcinogenesis, yet its precise functional roles and regulation remain unclear. In this study, we aim to investigate its biological function and clinical significance in human colon cancer. Methods We analyzed the expression of TIMP1 in both public database (Oncomine and TCGA) and 94 cases of...

Flavonoids in Juglans regia L. Leaves and Evaluation of In Vitro Antioxidant Activity via Intracellular and Chemical Methods

Flavonoids are rich in Juglans regia L. leaves. They have potent antioxidant properties, which have been related to regulating immune function and enhancing anticancer activity. Herein, qualitative and quantitative determination of flavonoids from J. regia leaves was carried out using high performance liquid chromatography coupled with tandem mass spectrometry with electrospray...

Secernin-1 Contributes to Colon Cancer Progression through Enhancing Matrix Metalloproteinase-2/9 Exocytosis

cell invasion and tumor metastasis. Conflict of Interests The authors declare that there is no conflict of interests regarding the publication of this paper. Authors’ Contribution Shengtao Lin and Tao ... Jiang contributed equally to this work. Acknowledgments This research was supported by the National Natural Science Foundation (Grants 30901423 and 81272744, to Fan Junwei), a grant funded by the Chinese

Detection of Homologous Recombination Events in Bacterial Genomes

We study the detection of mutations, sequencing errors, and homologous recombination events (HREs) in a set of closely related microbial genomes. We base the model on single nucleotide polymorphisms (SNPs) and break the genomes into blocks to handle the rearrangement problem. Then we apply a dynamic programming algorithm to model whether changes within each block are likely a...

A single aspergillus fumigatus intracranial abscess in an immunocompetent patient with parietal lobe tumorectomy

Aspergillosis of the central nervous system is a rare fungal infection that is mainly reported in patients with immune deficiency, such as AIDS patients and organ transplant patients treated with immunosuppressive agents, and is uncommon among patients with intact immune function. We report here a rare case of intracranial aspergillosis in a patient who had previously undergone a...

Transcriptome assembly and isoform expression level estimation from biased RNA-Seq reads

Motivation: RNA-Seq uses the high-throughput sequencing technology to identify and quantify transcriptome at an unprecedented high resolution and low cost. However, RNA-Seq reads are usually not uniformly distributed and biases in RNA-Seq data post great challenges in many applications including transcriptome assembly and the expression level estimation of genes or isoforms. Much...

Mitochondrial CB1 receptor is involved in ACEA-induced protective effects on neurons and mitochondrial functions

Hospital, The Fourth Military Medical University, Xi’an, Shaanxi, China, 710032Lei Ma, Ji Jia, Tao Jiang, Qian Zhai, Lei Yang, Fuhai Bai, Qiang Wang & Lize XiongDepartment of Pathology and Pathophysiology ... • PubMed • Google ScholarSearch for Wen Niu in:Nature Research journals • PubMed • Google ScholarSearch for Tao Jiang in:Nature Research journals • PubMed • Google ScholarSearch for Qian Zhai in:Nature