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Assessing characteristics of RNA amplification methods for single cell RNA sequencing

Background Recently, measurement of RNA at single cell resolution has yielded surprising insights. Methods for single-cell RNA sequencing (scRNA-seq) have received considerable attention, but the broad reliability of single cell methods and the factors governing their performance are still poorly known. Results Here, we conducted a large-scale control experiment to assess the ...

Capsule Design for Blue Light Therapy against Helicobacter pylori

A photo-medical capsule that emits blue light for Helicobacter pylori treatment was described in this paper. The system consists of modules for pH sensing and measuring, light-emitting diode driver circuit, radio communication and microcontroller, and power management. The system can differentiate locations by monitoring the pH values of the gastrointestinal tract, and turn on and ...

Collapsing singletons may boost signal for associating rare variants in sequencing study

Advances in next-generation sequencing technology have made it possible to comprehensively interrogate the entire spectrum of genomic variations including rare variants. They may help capture the remaining genetic heritability which has not been fully explained by previous genome-wide association studies. Here we performed a gene-based genome-wide scan to identify hypertension ...

Placenta Growth Factor in Eyes with Neovascular Glaucoma Is Decreased after Intravitreal Ranibizumab Injection

Purpose To evaluate changes in the concentrations of placental growth factor (PlGF) and vascular endothelial growth factor-A (VEGF-A) in aqueous humor of patients with neovascular glaucoma (NVG) before and after an intravitreal injection of ranibizumab (IVR) and to determine the underlying correlation between the levels. Methods The prospective interventional comparative study ...

Multiplex cytokine levels of aqueous humor in acute primary angle-closure patients: fellow eye comparison

Background The existing literature contains no information regarding inflammatory cytokine expression in unilateral acute primary angle-closure (APAC) affected eyes and fellow eyes with primary angle closure suspect (PACS). To measure levels of various inflammatory cytokines in the aqueous humor (AH) of APAC affected eyes and fellow eyes with a diagnosis of PACS (18 unilateral APAC ...

Preliminary proposal of scientific data verification in CSES mission

China Seismo-Electromagnetic Satellite (CSES) will be launched at the end of 2016 and the orbit is sun-synchronous and the altitude is about 500 km. The design of CSES satellite and ground segment are introduced in this paper first. And then the preliminary proposals of scientific data verification and cross-verification in CSES mission are given, which can be used to classify the ...

Insights into the function of tegument proteins from the varicella zoster virus

Chickenpox (varicella) is caused by primary infection with varicella zoster virus (VZV), which can establish long-term latency in the host ganglion. Once reactivated, the virus can cause shingles (zoster) in the host. VZV has a typical herpesvirus virion structure consisting of an inner DNA core, a capsid, a tegument, and an outer envelope. The tegument is an amorphous layer ...

Copy number variation of a gene cluster encoding endopolygalacturonase mediates flesh texture and stone adhesion in peach

Texture is an important attribute affecting consumer perception of fruit quality. Peach melting flesh and flesh adhesion to stone (endocarp) are simply inherited and controlled by the F-M locus on linkage group (LG) 4. Here, we report that two genes encoding endopolygalacturonase (endoPG) in the F-M locus, designated PpendoPGF and PpendoPGM, are associated with the melting flesh ...

A facile method to fabricate Al4B2O9 whiskers on porous SiC substrates for gas–solid separation

Al4B2O9 whiskers on the surface of porous SiC substrates were fabricated by chemical reactions between Al(NO3)3 and H3BO3, which is a facile method to prepare cilia-like microstructure for gas–solid separation. Scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy were employed to investigate the structural morphology and ...

Renal vein thrombosis mimicking urinary calculus: a dilemma of diagnosis

Background Renal vein thrombosis (RVT) with flank pain, and hematuria, is often mistaken with renal colic originating from ureteric or renal calculus. Especially in young and otherwise healthy patients, clinicians are easily misled by clinical presentation and calcified RVT. Case presentation A 38-year-old woman presented with flank pain and hematuria suggestive of renal calculus ...

L p Blaschke-Minkowski homomorphisms

In this paper, we introduce the concept of Lp Blaschke-Minkowski homomorphisms and show that those maps are represented by a spherical convolution operator. And then we consider the Busemann-Petty type problem for Lp Blaschke-Minkowski homomorphisms.MSC: 52A40, 52A20.

Relationship between Chinese adjective descriptors of personality and emotional symptoms in young Chinese patients with bipolar disorders

Objective To investigate whether personality traits are related to emotional symptoms (mania, hypomania, and depression) in Chinese patients with bipolar disorders.

Detection of the rs10250202 polymorphism in protection of telomeres 1 gene through introducing a new restriction enzyme site for PCR–RFLP assay

Human protection of telomeres 1 (POT1) gene is a single stranded telomere binding proteins with a critical role in ensuring chromosome stability. There have been variants of POT1 gene, and the polymorphisms of POT1 gene were associated with some diseases. Polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) is a traditional method to detect the single ...

Event-related potentials elicited by the Deutsch “high-low” word illusion in the patients with first-episode schizophrenia with auditory hallucinations

Background The exact cerebral structural and functional mechanisms under the auditory verbal hallucinations (AVHs) in schizophrenia are still unclear. The Deutsch “high-low” word illusion might trigger attentional responses mimicking those under AVHs. Methods We therefore have invited 16 patients with first-episode, paranoid schizophrenia, and 16 age- and gender-matched healthy ...

Single nucleotide polymorphism rs13042395 in the SLC52A3 gene as a biomarker for regional lymph node metastasis and relapse-free survival of esophageal squamous cell carcinoma patients

Background SLC52A3 was recently identified as a susceptibility gene for esophageal squamous cell carcinoma (ESCC). However, associations between the single nucleotide polymorphisms (SNPs) rs13042395 (C > T) and rs3746803 (G > A) in SLC52A3 and risk, tumor characteristics and survival of ESCC patients remain inconclusive and of unknown prognostic significance. Methods Analyses of ...

A novel efficient β-glucanase from a paddy soil microbial metagenome with versatile activities

Background Cellulose, an abundant and renewable polysaccharides, constitutes the largest resource for bioconversion of biofuels. Plant polysaccharides hydrolysis is catalyzed by cellulases, which include endoglucanases, exoglucanases, and β-glucosidases. Converting cellulose and hemicellulose to short chains of oligosaccharides by endo-/exoglucanases is the key step for biofuel ...

Combination Therapy Using Chelating Agent and Zinc for Wilson’s Disease

There is no clear international consensus regarding the optimal medication therapy for treating Wilson’s disease (WD). This study systematically reviews the effectiveness of various medication therapies in common use, specifically focusing on preliminary findings concerning the combination of a chelating agent and zinc. A systematic PubMed search was executed to locate original ...