Tissue engineering using suitable mesenchymal stem cells (MSCs) shows great potential to regenerate bone defects. Our previous studies have indicated that human amnion-derived mesenchymal stem cells ... cells (HAMSCs) could promote the osteogenic differentiation of human bone marrow mesenchymal stem cells (HBMSCs). Human adipose-derived stem cells (HASCs), obtained from adipose tissue in abundance, are
The most efficient method to expand limbal stem cells (LSCs) in vitro for clinical transplantation is to culture single LSCs directly on growth-arrested mouse fibroblast 3T3 cells. To reduce possible
Mesenchymal stem cells (MSCs) are promising candidates for skin wound repair due to their capabilities of accumulating at wounds and differentiating into multiple types of skin cells. However, the ... orchestrates the reconstruction of dermal and epidermal layers. This process involves three overlapping phases, including the inflammatory, proliferation, and remodeling phases. Mesenchymal stem cells (MSCs) can
Use of stem cell-based therapies in tissue engineering and regenerative medicine is hindered by efficient means of directed differentiation. For pluripotent stem cells, an initial critical ... motor during spontaneous differentiation of embryonic stem cells in embryoid bodies. We found that inhibiting either actin polymerization or actin-myosin interactions led to a decrease in differentiation
Background Hypoxia preconditioning has been proven to be an effective method to enhance the therapeutic action of mesenchymal stem cells (MSCs). However, the beneficial effects of hypoxic MSCs in ... ; MSCs, mesenchymal stem cells; NF-kB, nuclear factor kappa-κB; Ppa, pulmonary arterial pressure; P38 MAPK, P38 mitogen-activated protein kinase; TNF-α, tumor necrosis factor-α; VCAM-1, vascular cell
stem cells (ASCs) exert potent anti-inflammatory properties. The goal of the present study was to test the safety and ecfficacy of ASCs, in a well-characterized clinically relevant ovine model of ARDS ... ]. Mesenchymal stem cells (MSCs) have been shown to be beneficial in many pathological conditions, such as myocardial infarction [ 4 ], graft versus host disease [ 5 ] and spinal cord injury [ 6 ]. Believed
Background Mesenchymal stem cells (MSCs) are promising cells for cardiovascular regenerative medicine. However, their potential may be limited, because of their restricted cardiovascular ... Gandhi Post-Graduate Institute of Medical Sciences , Lucknow , India 1 Editor: Johnson Rajasingh, University of Kansas Medical Center , UNITED STATES Mesenchymal stem cells (MSCs) are promising cells for
verify the potential role of Euglena consumption on human health and obesity, we evaluated the effect of Euglena on human adipose-derived stem cells. We prepared a Euglena extract and evaluated its effect ... viability were observed when these additives were used at high concentrations [ 25, 26 ]. However, studies on adipocyte -differentiation in human-derived stem cells have been limited. Thus, the development
respond. 1. Tan G , Cheng L , Chen T , Yu L , Tan Y ( 2014 ) Foxm1 Mediates LIF/Stat3-Dependent Self-Renewal in Mouse Embryonic Stem Cells and Is Essential for the Generation of Induced Pluripotent Stem ... Cells . PLoS ONE 9 ( 4 ): e92304. https://doi.org/10.1371/journal.pone. 0092304 PMID: 24743237
Background Donation of haematopoietic stem cells, either through bone marrow (BM) or peripheral blood stem cell (PBSC) collection, is a generally safe procedure for healthy donors, although side ... resilience that has been observed previously in haematopoietic stem cells donors. Significant feelings of grief were reported after a recipient's death. Possibilities to alleviate these symptoms may include
The highly proliferative and pluripotent characteristics of embryonic stem cells engender great promise for tissue engineering and regenerative medicine, but the rapid identification and isolation of ... embryonic stem cells stiffened within one day of differentiation induced by leukemia inhibitory factor removal, with a lagging but pronounced change from spherical to spindle-shaped cell morphology. A
The inner ear represents a promising system to develop cell-based therapies from human induced pluripotent stem cells (hiPSCs). In the developing ear, Notch signaling plays multiple roles in otic ... leading to permanent hearing loss. Over the past few years, stem cell-based therapy approaches aiming to emulate otic development in the production of HCs from stem cells have received substantial interest
of hPSC-cardiomyocytes (CMs) using defined media; however, the mechanisms involved with the inductive process are poorly understood. Using a genetically modified mouse embryonic stem cell (mESC) line ... competing interests exist. Introduction Human pluripotent stem cells (hPSCs) hold great promise for cell-replacement therapies and the treatment of human heart failure. The use of chemically defined media
Human bone marrow-derived mesenchymal stem cells (MSCs) have been observed to inhibit arthritis in experimental animal models such as collagen-induced arthritis. However, the exact anti-inflammatory ... of arthritis was scored by two independent examiners. 2 / 14 Mesenchymal stem cells MSC line (BM025SS13) was obtained from the Catholic Institute of Cell Therapy, South Korea. MSCs were cultured in
chondrocytes and human mesenchymal stem cells in a three-dimensional (3D)-bioprinted hydrogel scaffold. The 3D-bioprinted constructs (5 × 5 × 1.2 mm) were produced using nanofibrillated cellulose and alginate in ... combination with human chondrocytes and human mesenchymal stem cells using a 3D-extrusion bioprinter. Immediately following bioprinting, the constructs were implanted subcutaneously on the back of 48 nude mice
facilitates its inhibitory function by activation of Wnt signaling. Thus, lncRNAs modulate the fate decision of pluripotent stem cells. ... interests exist. Introduction Embryonic stem cells (ESC) are characterized by their ability of long-term self-renewal as well as their potential to differentiate into each cell type of the embryo proper
Recent studies have suggested a role for the Cyclin Dependent Kinase-2 Associated Protein 1 (CDK2AP1) in stem cell differentiation and self-renewal. In studies with mouse embryonic stem cells (mESCs ... stem cells (hESCs). Using a shRNA to reduce its expression in hESCs, we found that CDK2AP1 knockdown resulted in a significant reduction in the expression of the pluripotency genes, OCT4 and NANOG. We
differentiation of dental stem cells combined with the use of medical grade synthetic biomaterial is still under investigation. Thus, the optimal scaffold composition, cell type and specific growth conditions, yet ... and specific growth conditions, yet need to be formulated. In this study, we aimed to investigate the regenerative potential of dental stem cells seeded on synthetic scaffolds and maintained in
Whereas transformation events in hematopoietic malignancies may occur at different developmental stages, the initial mutation originates in hematopoietic stem cells (HSCs), creating a preleukemic ... progenitors are transformed in the thymic environment [ 30 ]. Hematopoietic stem cells (HSCs) are responsible for generation and maintenance of multiple lineages in the blood supply [ 31 ]. Recent evidence
