Newborn screening (NBS), such as tandem mass spectrometry (MS/MS), may yield false positive/negative results. Next-generation sequencing (NGS) has the potential to provide increased data output ... newborns in Huzhou, Zhejiang province, China and explore the applicability of NGS and MS/MS in NBS. Methods Blood spot samples from 1263 newborns were collected. NGS was employed to screen for pathogenic
, 28% [95% CI 22–34] of researchers (10 surveys, 2180 respondents) felt that this practice was ‘never’, 24% [95% CI 22–27] ‘rarely’, 25% [95% CI 23–28] ‘sometimes’, 13% [95% CI 9–17] ‘most of the time ... of the justification ‘always justified’ Review item 3a 28% [95% CI 22–34] (2180 respondents in 10 surveys) Review item 3a 24% [95% CI 22–27] (2180 respondents in 10 surveys) Review item 3a 25% [95
daidzein concentrations from the blood and urine of 292 men and 174 women aged between 22 and 88 years by liquid chromatography-tandem mass spectrometry (LC‒MS/MS). We then analysed the cut-off value for ... the administrative support for this study but the funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors received no
The analysis of ceramide (Cer) and sphingomyelin (SM) lipid species using liquid chromatography–tandem mass spectrometry (LC–MS/MS) continues to present challenges as their precursor mass and ... chromatograms. ReTimeML works on the principle that LC–MS/ MS experiments have pre-determined RTs from internal standards, calibrators or quality controls used throughout the analysis. Employed as reference RTs
dereplication in further studies. To overcome this limitation, we constructed a high-resolution tandem mass spectrometry (MS/MS) dataset comprising pure NEO-PUFAs (both commercial and self-synthesized) and in ... (MS). Over the past three decades, advances in hyphenated techniques such as liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) along with high resolution MS and novel data analysis
combined with liquid chromatography coupled to tandem mass spectrometry (AP–MS) represents the method of choice to identify interacting proteins. The composition of complex isoforms concurrently present in ... PPIs also identified by DIP-MS (Supplementary Fig. 3a,b). In summary, our benchmarking data indicate that DIP-MS data, when compared to SEC–MS or reciprocal AP–MS data, have a broader dynamic range
Modern untargeted mass spectrometry (MS) analyses quickly detect and resolve thousands of molecular compounds. Although features are readily annotated with a molecular formula in high-resolution
Breast cancer is a major health concern, and its accurate diagnosis and management depend on identifying its histological type and biological subtype. Semaphorin-3A (SEMA3A) is a membrane protein ... cores cut before. Then, slides were baked for at 60 min in 60 °C. A Semaphorin 3A, clone EPR19367 was used to demonstrate expression of the secreted protein. Deparaffinization, rehydration and heat
. Herein, we explored metabolites associated with sarcopenia by untargeted gas chromatography (GC)/liquid chromatography (LC)–mass spectrometry (MS) and identified possible diagnostic markers. Forty-eight ... . Methods Forty-eight elderly subjects with sarcopenia were age and sex matched with 48 elderly subjects without sarcopenia. We first used untargeted GC/LC-MS to analyze the plasma of these participants and
In common with other omics technologies, mass spectrometry (MS)-based proteomics produces ever-increasing amounts of raw data, making efficient analysis a principal challenge. A plethora of different ... plethora of different computational tools can process the MS data to derive peptide and protein identification and quantification. However, during the last years there has been dramatic progress in computer
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missed the optimal timing for treatment. The aim of the research is to study the serum metabolomics of Chinese LN patients using gas chromatography (GC)/mass spectrometry (MS) and liquid chromatography (LC ... (BMI) and age, were selected. Serum analysis was conducted using GC/MS and LC/MS, followed by multivariate statistical analysis. Various multidimensional analyses, including principal component analysis
) produced 1-((aryldiazenyl) cyanoacetyl piperidines (3a–d). To create the derivatives of 4-(phenyldiazenyl)-5-(piperidin-1-yl)-1H-pyrazol-3-amine (5a), the propane nitriles (3a–d) were condensed with ... dyes (4a-d) were identified as the reaction results. The MS, IR, and NMR spectra were used to describe the novel dyes, and the results exactly matched the suggested structures. The antibacterial test
untargeted metabolomics and lipidomics analysis was performed in IS of asthmatic (n = 15) and COPD (n = 22) patients based on Ultra-High-Pressure Liquid Chromatography-Mass Spectrometry (UHPLC-MS) and UHPLC ... COPD and asthmatic patients. Among the 15 selected candidate biomarkers based on Variable Importance in Projection scores, putrescine showed the highest score. A differential IS bio-signature of 22
diazepines (3a–d) or with 2-aminophenol to offer oxazepine (4a–d). The structure of the synthesized compounds was confirmed via melting points, elemental analyses, and different spectroscopic techniques ... anticancer potential. Our results showed that, diazepine (3a) and oxazepine (4a) offered the highest binding energy against the target OMPA/ exo‑1,3‑beta‑glucanase proteins and exhibited the potent
position 3 and 7 were synthesized and screened for cytotoxic activity utilizing 60 different human cancer cell lines. Furthermore, compounds 3a,b,c,e that revealed potent broad-spectrum anticancer activity ... arrest at the G2-M phase. Moreover, the mechanism of action was determined by determining the topoisomerase inhibitory activity and the molecular modeling study. Compounds 3a,b,c,e showed broad spectrum
this study was to establish a rapid and reliable method for the detection of abexinostat concentrations in rat plasma using ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS ... ; Pharmacokinetics; UPLC‑MS/MS; Givinostat - †Yige Yu and Jinyu Hu contributed equally to this work. 3]. Previous studies have shown that increased abexinostat concentration promoted apoptosis in Hodgkin lymphoma
accurate, quantitative methodology for measuring NMN by using liquid chromatography-triple quadrupole mass spectrometry (LC-MS/MS) with double isotopic NMN standards. In this new methodology, the matrix ... samples has long been debated. Several groups have been using their own methodologies with liquid chromatography-tandem mass spectrometry (LC-MS/MS) to detect and measure NMN in biological samples18–22
, Esatbeyoglu T, Oz F, Abd El-Aty AM (2023) Development of an enhanced analytical method utilizing pepper matrix as an analyte protectant for sensitive GC–MS/MS detection of dimethipin in animal-based food
