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8 papers found.
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NMD monitors translational fidelity 24/7

Nonsense-mediated mRNA decay (NMD) is generally thought to be a eukaryotic mRNA surveillance pathway tasked with the elimination of transcripts harboring an in-frame premature termination codon (PTC). As presently conceived, NMD acting in this manner minimizes the likelihood that potentially toxic polypeptide fragments would accumulate in the cytoplasm. This notion is to be...

Degradation of YRA1 Pre-mRNA in the Cytoplasm Requires Translational Repression, Multiple Modular Intronic Elements, Edc3p, and Mex67p

Mex67p Shuyun Dong 0 Allan Jacobson 0 Feng He 0 Marv Wickens, University of Wisconsin, United States of America 0 Department of Molecular Genetics and Microbiology, University of Massachusetts Medical

Poly(A)-binding proteins: multifunctional scaffolds for the post-transcriptional control of gene expression

: Allan Jacobson. E-mail: 0 0 Address: Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School , 55 Lake Avenue North, Worcester, MA 01655-0122 , USA Most eukaryotic

Possible Involvement of poly(A) in protein synthesks

The experiments of this paper have re-evaluated the possibility that poly(A) is involved in protein synthesis by testing whether purified poly(A) might competitively inhibit in vitro protein synthesis in rabbit reticulocyte extracts. We have found that poly(A) inhibits the rate of translation of many different poly(A)+ mRNAs and that comparable inhibition is not observed with...

Analysis of chimeric mRNAs derived from the STE3 mRNA identifies multiple regions within yeast mRNAs that modulate mRNA decay

In the yeast Saccharomyces cerevlslae unstable mRNAs decay 10 – 20 fold more rapidly than stable mRNAs. In order to examine the basis for the differences in decay rate of the unstable STE3 mRNA and the stable PGK1 and ACT1 mRNAs we have constructed and measured the decay rates of numerous chimeric mRNAs. These experiments indicate that multiple regions within yeast mRNAs are...

Intron bypass: a rapid procedure for eliminating introns from cloned genomic DNA and its application to a cellulase gene

We have devised a DNA cloning procedure in which the introns present in a genomic DNA fragment can be eliminated easily and rapidly. The technique combines the methods of cDNA and genomic cloning in a way which assures full-length representation of the intron-free transcript. Moreover, plasmids made by this technique can be designed to contain flanking untranscribed regions which...

All-night EEG sleep measurements in young adults

The EEG sleep characteristics of normal, young adults were measured for three consecutive nights. The mean percentage of Stages 1-REM, 3 and 4 showed an increase with each consecutive night. Within a night’s sleep, Stages 3 and 4 decreased markedly and Stage 1-REM increased considerably from the first to the second half of the night. The mean duration of REMPs increased from the...