Education and Research (ZBAF), Department of Human Medicine, Faculty of Health, University Witten/ Herdecke , Witten , Germany 1 & Anja Ehrhardt 2 & Florian Kreppel 3 Chair for Virology and Microbiology
author publications You can also search for this author in PubMed Google Scholar Anja Ehrhardt View author publications You can also search for this author in PubMed Google Scholar Contributions KM ... Correspondence to Anja Ehrhardt. Ethics declarations Ethics approval and consent to participate Not applicable. Consent for publication All authors agreed to the publication of this manuscript. Competing
Florian Kreppel for helpful scientific discussions. 10 / 12 Author Contributions Conceptualization: Oskar Bunz, Kemal Mese, Anja Ehrhardt. Funding acquisition: Oskar Bunz. Methodology: Oskar Bunz, Kemal ... Mese, Wenli Zhang. Resources: Wenli Zhang. Supervision: Andree Piwowarczyk, Anja Ehrhardt. Writing ? original draft: Oskar Bunz, Anja Ehrhardt. Writing ? review & editing: Andree Piwowarczyk. 11 / 12 1
Wang Chuanbo Sun Maren Gebbing Csaba Miskey Zoltan Ivics Zsuzsanna Izsvak Anja Ehrhardt OPEN ACCESS The following information is missing from the Funding statement: Dr. Zsuzsanna Izsva´k was funded by
0 Anja Ehrhardt 0 0 Institute of Virology and Microbiology, Center for Biomedical Education and Research (ZBAF), Department of OPEN The Clustered Regularly Interspaced Short Palindromic Repeats
Background Integrating non-viral vectors based on transposable elements are widely used for genetically engineering mammalian cells in functional genomics and therapeutic gene transfer. For the Sleeping Beauty (SB) transposase system it was demonstrated that convergent transcription driven by the SB transposase inverted repeats (IRs) in eukaryotic cells occurs after somatic...
Ehrhardt 1 2 0 Institute of Virology, Albert-Ludwigs-University Freiburg , Freiburg , Germany 1 Institute of Virology and Microbiology, Center for Biomedical Education and Research (ZBAF), Department of
• Google Scholar Search for Anja Ehrhardt in:Nature Research journals • PubMed • Google Scholar Contributions J.L. and A.E. jointly conceived the study. J.L. performed the majority of experiments. J.F ... the manuscript. Competing Interests The authors declare no competing interests. Corresponding author Correspondence to Anja Ehrhardt. Electronic supplementary material Supplementary Information
RNA interference (RNAi) is a key regulator of various biological systems including viral infection. Within a virus life cycle gene products can be modulated by the RNA interference (RNAi) pathway which can crucially impact productive virus replication. Herein we explored the RNA interference suppressor protein P19 derived from a plant virus and we found that P19 enhanced...
Recombinant adeno-associated viral (AAV) vectors have been shown to be one of the most promising vectors for therapeutic gene delivery because they can induce efficient and long-term transduction in non-dividing cells with negligible side-effects. However, as AAV vectors mostly remain episomal, vector genomes and transgene expression are lost in dividing cells. Therefore, to...
We recently developed adenovirus/transposase hybrid-vectors utilizing the previously described hyperactive Sleeping Beauty (SB) transposase HSB5 for somatic integration and we could show stabilized transgene expression in mice and a canine model for hemophilia B. However, the safety profile of these hybrid-vectors with respect to vector dose and genotoxicity remains to be...
Adenoviral gene therapy and oncolysis would critically benefit from targeted cell entry by genetically modified capsids. This requires both the ablation of native adenovirus tropism and the identification of ligands that remain functional in virus context. Here, we establish cell type-specific entry of HAdV-5-based vectors by genetic ligand insertion into a chimeric fiber with...
