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A Large-Scale Complex Haploinsufficiency-Based Genetic Interaction Screen in Candida albicans: Analysis of the RAM Network during Morphogenesis

The morphogenetic transition between yeast and filamentous forms of the human fungal pathogen Candida albicans is regulated by a variety of signaling pathways. How these pathways interact to orchestrate morphogenesis, however, has not been as well characterized. To address this question and to identify genes that interact with the Regulation of Ace2 and Morphogenesis (RAM...

A question of size: the eukaryotic proteome and the problems in defining it

We discuss the problems in defining the extent of the proteomes for completely sequenced eukaryotic organisms (i.e. the total number of protein-coding sequences), focusing on yeast, worm, fly and human. (i) Six years after completion of its genome sequence, the true size of the yeast proteome is still not defined. New small genes are still being discovered, and a large number of...

Analysis of small nucleolar RNAs reveals unique genetic features in malaria parasites

Prakash Chandra Mishra 0 Anuj Kumar 0 Amit Sharma 0 0 Address: Structural and Computational Biology Group, International Centre for Genetic Engineering and Biotechnology Aruna Asaf Ali Road , New

Characterization of W p -type of spaces involving fractional Fourier transform

The characterizations of W p -type of spaces and mapping relations between W- and W p -type of spaces are discussed by using the fractional Fourier transform. The uniqueness of the Cauchy problems is also investigated by using the same transform. MSC: 46F12, 46E15.

Complex dynamic of plankton–fish interaction with quadratic harvesting and time delay

The present paper deals with a food chain model consisting of three species phytoplankton, zooplankton and fish. We have divided the present paper into two parts. In the first part, we have assumed that the fish population is harvested using a non-linear harvesting function. Considering this rate of harvesting ‘E’ as a control parameter, we have estimated different ranges of...

Development of Monoclonal Antibodies against CMP-N-Acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1 (ST3Gal-I) Recombinant Protein Expressed in E. coli

; Revised 21 November 2015; Accepted 25 November 2015 Academic Editor: J. Justin Hsuan Copyright © 2015 Anuj Kumar Gupta et al. This is an open access article distributed under the Creative Commons

Enriched whole genome sequencing identified compensatory mutations in the RNA polymerase gene of rifampicin-resistant Mycobacterium leprae strains

Anuj Kumar Gupta,2 Madhvi Ahuja,1 Vinay Pathak,1 Utpal Sengupta,1 1Stanley Browne Laboratory, The Leprosy Mission Trust India, TLM Community Hospital Nand Nagari, 2Agilent Technologies India Pvt Ltd

Epigenetic Activation of Antibacterial Property of an Endophytic Streptomyces coelicolor Strain AZRA 37 and Identification of the Induced Protein Using MALDI TOF MS/MS

The endophytic Streptomyces coelicolor strain AZRA 37 was isolated from the surface sterilized root of Azadirachta indica A. Juss., commonly known as neem plant in India. Since only a few reports are available regarding epigenetic modulations of microbial entities, S. coelicolor was treated with different concentrations of 5-azacytidine for this purpose and evaluated for its...

Genome-wide identification and characterization of gene family for RWP-RK transcription factors in wheat (Triticum aestivum L.)

Data curation: Anuj Kumar, Ritu Batra, Sandhya Tyagi. Formal analysis: Anuj Kumar, Ritu Batra, Vijay Gahlaut, Tinku Gautam, Sanjay Kumar, Mansi Sharma, Krishna Pal Singh. Methodology: Renu Pandey ... . Supervision: Harindra Singh Balyan, Renu Pandey, Pushpendra Kumar Gupta. Writing – original draft: Anuj Kumar, Ritu Batra. Writing – review & editing: Harindra Singh Balyan, Renu Pandey, Pushpendra Kumar

Genomic analysis of insertion behavior and target specificity of mini-Tn7 and Tn3 transposons in Saccharomyces cerevisiae

Transposons are widely employed as tools for gene disruption. Ideally, they should display unbiased insertion behavior, and incorporate readily into any genomic DNA to which they are exposed. However, many transposons preferentially insert at specific nucleotide sequences. It is unclear to what extent such bias affects their usefulness as mutagenesis tools. Here, we examine...

Industrial enzymes in bioindustrial sector development: An Indian perspective

, Canada, India and Malaysia 1 Anuj Kumar Chandel has an MSc and is currently pursuing a PhD in biotechnology at the Jawaharlal Nehru Technological University. He is also working with Celestial Labs Ltd

Influence of alumina/MWCNT hybrid nanoparticle additives on tribological properties of lubricants in turning operations

27.36%, respectively, as compared to alumina-based lubricants. hybrids; nanolubricants; MQL; MWCNT; tool wear; friction coefficient - Anuj Kumar SHARMA*, Jitendra Kumar KATIYAR, Shubrajit BHAUMIK ... , provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Anuj Kumar SHARMA. He received his PhD

Monoclonal Antibodies against Hepatitis C Genotype 3a Virus Like Particle Inhibit Virus Entry in Cell Culture System

Department of Biotechnology, Govt. of India. Dr. Soma Das was the recipient of S K Kothari postdoctoral Fellowship from UGC, Govt. of India. Anuj Kumar Dayal was a recipient of Junior Research Fellowship from

Organelle DB: a cross-species database of protein localization and function

Nuwee Wiwatwattana 0 Anuj Kumar 0 0 Department of Molecular, Cellular, and Developmental Biology and Life Sciences Institute, University of Michigan , Ann Arbor, MI 48109-2216, USA To efficiently

Organelle DB: an updated resource of eukaryotic protein localization and function

Organelle DB (http://organelledb.lsi.umich.edu) is a web-accessible relational database presenting a supplemented catalog of organelle-localized proteins and major protein complexes. Since its release in 2004, Organelle DB has grown by 20% to encompass over 30 000 proteins from 138 eukaryotic organisms. Each protein in Organelle DB is presented with its subcellular localization...

Phylogenetic and Preliminary Phenotypic Analysis of Yeast PAQR Receptors: Potential Antifungal Targets

Proteins belonging to the Progestin and AdipoQ Receptor (PAQR) superfamily of membrane bound receptors are ubiquitously found in fungi. Nearly, all fungi possess two evolutionarily distinct paralogs of PAQR protein, which we have called the PQRA and PQRB subtypes. In the model fungus Saccharomyces cerevisiae, these subtypes are represented by the Izh2p and Izh3p proteins...

Pooled Segregant Sequencing Reveals Genetic Determinants of Yeast Pseudohyphal Growth

The pseudohyphal growth response is a dramatic morphological transition and presumed foraging mechanism wherein yeast cells form invasive and surface-spread multicellular filaments. Pseudohyphal growth has been studied extensively as a model of conserved signaling pathways controlling stress responses, cell morphogenesis, and fungal virulence in pathogenic fungi. The genetic...

SARS-CoV 9b Protein Diffuses into Nucleus, Undergoes Active Crm1 Mediated Nucleocytoplasmic Export and Triggers Apoptosis When Retained in the Nucleus

Background 9b is an accessory protein of the SARS-CoV. It is a small protein of 98 amino acids and its structure has been solved recently. 9b is known to localize in the extra-nuclear region and has been postulated to possess a nuclear export signal (NES), however the role of NES in 9b functioning is not well understood. Principal Findings/Methodology In this report, we...

Structure, localization and histone binding properties of nuclear-associated nucleosome assembly protein from Plasmodium falciparum

Background Nucleosome assembly proteins (NAPs) are histone chaperones that are crucial for the shuttling and incorporation of histones into nucleosomes. NAPs participate in the assembly and disassembly of nucleosomes thus contributing to chromatin structure organization. The human malaria parasite Plasmodium falciparum contains two nucleosome assembly proteins termed PfNapL and...