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11 papers found.
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Ag2O3 clathrate is a novel and effective antimicrobial agent

Silver compounds and silver ions are used extensively in medical devices because of their wide-spectrum antimicrobial activity. In particular, nanoparticles of silver and silver (I) oxide show great promise for widespread usage in medical polymers and nanodrugs. Here, we demonstrate that a crystalline powder and a saturated aqueous solution of silver (III) oxide clathrate show much ...

Isolation of a novel mutant gene for soil-surface rooting in rice (Oryza sativa L.)

Background Root system architecture is an important trait affecting the uptake of nutrients and water by crops. Shallower root systems preferentially take up nutrients from the topsoil and help avoid unfavorable environments in deeper soil layers. We have found a soil-surface rooting mutant from an M2 population that was regenerated from seed calli of a japonica rice cultivar, ...

Mapping and characterization of quantitative trait loci for mesocotyl elongation in rice (Oryza sativa L.)

a Scientific Research grant for a foreign student (Hyun-Sook Lee) from the Ministry of Education, Culture and Science, Japan. The authors thank Dr. Atsushi Higashitani (Graduate School of Life

Caenorhabditis elegans p97 controls germline-specific sex determination by controlling the TRA-1 level in a CUL-2-dependent manner

Yohei Sasagawa Mieko Otani Nahoko Higashitani Atsushi Higashitani Ken Sato Teru Ogura Kunitoshi Yamanaka - Summary p97 (CDC-48 in Caenorhabditis elegans) is a ubiquitin-selective AAA (ATPases

The Effectiveness of RNAi in Caenorhabditis elegans Is Maintained during Spaceflight

Background Overcoming spaceflight-induced (patho)physiologic adaptations is a major challenge preventing long-term deep space exploration. RNA interference (RNAi) has emerged as a promising therapeutic for combating diseases on Earth; however the efficacy of RNAi in space is currently unknown. Methods Caenorhabditis elegans were prepared in liquid media on Earth using standard ...

Radiation Biology of Caenorhabditis elegans: Germ Cell Response, Aging and Behavior

Tetsuya SAKASHITA Takako TAKANAMI Sumino YANASE Nobuyuki HAMADA Michiyo SUZUKI Takafumi KIMURA Yasuhiko KOBAYASHI Naoaki ISHII Atsushi HIGASHITANI Nematode/Germ line/Oxidative stress/Nervous

Effects of Ionizing Radiation on Locomotory Behavior and Mechanosensation in Caenorhabditis elegans

Michiyo SUZUKI Tetsuya SAKASHITA Sumino YANASE Masahiro KIKUCHI Hirofumi OHBA Atsushi HIGASHITANI Nobuyuki HAMADA Tomoo FUNAYAMA Kana FUKAMOTO Toshio TSUJI Yasuhiko KOBAYASHI Nematode/Behavior

Filamentous phage replication initiator protein gpII forms a covalent complex with the 5′ end of the nick it introduced

Rolling circle type DNA replication is initiated by introduction of a nick in the leading strand of the origin by the initiator protein, which in most cases binds covalently to the 5′ end of the nick. In filamentous phage, however, such a covalent complex has not been detected. Using a suitable substrate and short reaction time, we show that filamentous phage initiator gpII forms a ...

Hyper-resistance of meiotic cells to radiation due to a strong expression of a single recA-like gene in Caenorhabditis elegans

Sensitivity of meiotic cells to DNA damaging agents is little understood. We have demonstrated that the meiotic pachytene nuclei in the Caenorhabditis elegans gonad are hyper-resistant to X-ray irradiation, but not to UV irradiation, whereas the early embryonic cells after fertilization and the full grown oocytes are not. The Ce-rdh-1 gene [RAD51, DMC1 (LIM15), homolog 1 or ...

Efficient Repair of DNA Damage Induced by Heavy Ion Particles in Meiotic Prophase I Nuclei of Caenorhabditis elegans


A single amino acid substitution reduces the superhelicity requirement of a replication initiator protein

The origin of rolling circle replication in filamentous coliphage consists of a core origin that is absolutely required and an adjacent replication enhancer sequence that increases in vivo replication 30 to 100-fold. The core origin binds the initiator protein (gpII) which either nicks or relaxes negatively superhelical replicative form DNA (RFI). Nicking at the origin, but not ...