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The nuclear proteome of Trypanosoma brucei

Wuƈrzburg, Germany) for mentoring and hosting the work. Author Contributions Conceptualization: Christian J. Janzen, Falk Butter, Susanne Kramer. Data curation: Carina Goos, Mario Dejung. Formal analysis ... : Mario Dejung, Falk Butter. Funding acquisition: Susanne Kramer. Investigation: Carina Goos. Methodology: Carina Goos, Mario Dejung, Falk Butter. Project administration: Christian J. Janzen, Falk

Novel insights into RNP granules by employing the trypanosome's microtubule skeleton as a molecular sieve

RNP granules are ribonucleoprotein assemblies that regulate the post-transcriptional fate of mRNAs in all eukaryotes. Their exact function remains poorly understood, one reason for this is that RNP granule purification has not yet been achieved. We have exploited a unique feature of trypanosomes to prepare a cellular fraction highly enriched in starvation stress granules. First...