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29 papers found.
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Old Master Zhu: in memory of virologist Guan-Fu Zhu

Qin 0 1 0 Born in Dinghai County of Zhejiang province in June , 1926 , Guan-Fu Zhu began to study at School of Medicine, Tongji University , in October, 1946 (Fig. 1). Then he moved to the Department

Characterization of a candidate tetravalent vaccine based on 2'-O-methyltransferase mutants

, Melissa Chng, Ying Xiu Toh, Jing Zou, Hongping Dong, Chao Shan, Jassia Pang. Project administration: Jassia Pang, Pei-Yong Shi, Katja Fink. Resources: Cheng-Feng Qin, Evan W. Newell, Pei-Yong Shi, Katja ... Fink. Supervision: Cheng-Feng Qin, Evan W. Newell, Pei-Yong Shi, Katja Fink. Validation: Roland ZuÈst. Visualization: Roland ZuÈst, Katja Fink. Writing ± original draft: Roland ZuÈst, Melissa Chng

Epidemiological and Virological Characterizations of the 2014 Dengue Outbreak in Guangzhou, China

Dengue used to be recognized as an imported and sporadic disease in China. Since June 2014, an unexpected large dengue outbreak has attacked Guangzhou, China, resulting in more than 40,000 cases. Among the 1,942 laboratory-confirmed hospitalized dengue cases, 121 were diagnosed as severe dengue according to the 2009 WHO guideline, and 2 patients finally died. Laboratory diagnosis...

Human Enterovirus Nonstructural Protein 2CATPase Functions as Both an RNA Helicase and ATP-Independent RNA Chaperone

RNA helicases and chaperones are the two major classes of RNA remodeling proteins, which function to remodel RNA structures and/or RNA-protein interactions, and are required for all aspects of RNA metabolism. Although some virus-encoded RNA helicases/chaperones have been predicted or identified, their RNA remodeling activities in vitro and functions in the viral life cycle remain...

Characterization of a Novel Dengue Serotype 4 Virus-Specific Neutralizing Epitope on the Envelope Protein Domain III

The dengue virus (DENV) envelope protein domain III (ED3) has been suggested to contain receptor recognition sites and the critical neutralizing epitopes. Up to date, relatively little work has been done on fine mapping of neutralizing epitopes on ED3 for DENV4. In this study, a novel mouse type-specific neutralizing antibody 1G6 against DENV4 was obtained with both prophylactic...

Isolation, identification and genomic characterization of the Asian lineage Zika virus imported to China

the convalescent serum from recovered Zika patient. This work was supported by the State Key Laboratory of Pathogen and Biosecurity, China. Cheng-Feng Qin was supported by the Excellent Young Scientist

Development of a Double Antibody Sandwich ELISA for West Nile Virus Detection Using Monoclonal Antibodies against Non-Structural Protein 1

The early diagnosis of West Nile virus (WNV) infection is important for successful clinical management and epidemiological control. The non-structural protein 1 (NS1) of flavivirus, a highly conserved and secreted glycoprotein, is abundant in the serum of flavivirus-infected patients and represents a useful early diagnostic marker. We developed a WNV-specific NS1 antigen-capture...

Dengue Type Four Viruses with E-Glu345Lys Adaptive Mutation from MRC-5 Cells Induce Low Viremia but Elicit Potent Neutralizing Antibodies in Rhesus Monkeys

Knowledge of virulence and immunogenicity is important for development of live-attenuated dengue vaccines. We previously reported that an infectious clone-derived dengue type 4 virus (DENV-4) passaged in MRC-5 cells acquired a Glu345Lys (E-E345K) substitution in the E protein domain III (E-DIII). The same cloned DENV-4 was found to yield a single E-Glu327Gly (E-E327G) mutation...

Recombinant tandem multi-linear neutralizing epitopes of human enterovirus 71 elicited protective immunity in mice

Background Human Enterovirus 71 (EV71) has emerged as the leading cause of viral encephalitis in children, especially in the Asia-Pacific regions. EV71 vaccine development is of high priority at present, and neutralization antibodies have been documented to play critical roles during in vitro and in vivo protection against EV71 infection. Results In this study, a novel strategy...

The Self-Interaction of a Nodavirus Replicase Is Enhanced by Mitochondrial Membrane Lipids

RNA replication of positive-strand (+)RNA viruses requires the protein-protein interactions among viral replicases and the association of viral replicases with intracellular membranes. Protein A from Wuhan nodavirus (WhNV), which closely associate with mitochondrial membranes, is the sole replicase required for viral RNA replication. Here, we studied the direct effects of...

A cypovirus VP5 displays the RNA chaperone-like activity that destabilizes RNA helices and accelerates strand annealing

For double-stranded RNA (dsRNA) viruses in the family Reoviridae, their inner capsids function as the machinery for viral RNA (vRNA) replication. Unlike other multishelled reoviruses, cypovirus has a single-layered capsid, thereby representing a simplified model for studying vRNA replication of reoviruses. VP5 is one of the three major cypovirus capsid proteins and functions as a...

Induction of Neutralizing Antibodies against Four Serotypes of Dengue Viruses by MixBiEDIII, a Tetravalent Dengue Vaccine

The worldwide expansion of four serotypes of dengue virus (DENV) poses great risk to global public health. Several vaccine candidates are under development. However, none is yet available for humans. In the present study, a novel strategy to produce tetravalent DENV vaccine based on envelope protein domain III (EDIII) was proposed. Tandem EDIIIs of two serotypes (type 1–2 and...

Development of reverse-transcription loop-mediated isothermal amplification assay for rapid detection of novel avian influenza A (H7N9) virus

Background The emerged human infection with avian influenza A (H7N9) virus in China since 2013 has aroused global concerns. There is great demand for simple and rapid diagnostic method for early detection of H7N9 to provide timely treatment and disease control. The aim of the current study was to develop a rapid, accurate and feasible reverse-transcription loop-mediated...

In Vitro Characterization of Human Adenovirus Type 55 in Comparison with Its Parental Adenoviruses, Types 11 and 14

recombinant HAdV, shedding light on the pathogenesis of HAdV-B55. - Funding: This work was supported by the National Basic Research Project of China (Grant 2012CB518904). Cheng-Feng Qin was supported by the

Parallel mRNA and MicroRNA Profiling of HEV71-Infected Human Neuroblastoma Cells Reveal the Up-Regulation of miR-1246 in Association with DLG3 Repression

Human enterovirus 71 (HEV71) has emerged as the leading cause of viral encephalitis in children in most Asian countries. The roles of host miRNAs in the neurological pathogenesis of HEV71 infection remain unknown. In the present study, comprehensive miRNA expression profiling in HEV71-infected human neuroblastoma SH-SY5Y cells was performed using the Affymetrix Gene Chip...

A novel reporter system for neutralizing and enhancing antibody assay against dengue virus

-Ya Zhu 0 Pei-Yong Shi Bo Zhang Fu-Chun Zhang E-De Qin 0 Cheng-Feng Qin 0 0 Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology

Development and characterization of the replicon system of Japanese encephalitis live vaccine virus SA14-14-2

Shi-Hua Li 0 Xiao-Feng Li 0 Hui Zhao Yong-Qiang Deng Xue-Dong Yu Shun-Ya Zhu Tao Jiang Qing Ye E-De Qin Cheng-Feng Qin 0 Equal contributors Department of Virology, State Key Laboratory of Pathogen

TLR3 Signaling in Macrophages Is Indispensable for the Protective Immunity of Invariant Natural Killer T Cells against Enterovirus 71 Infection

Enterovirus 71 (EV71) is the most virulent pathogen among enteroviruses that cause hand, foot and mouth disease in children but rarely in adults. The mechanisms that determine the age-dependent susceptibility remain largely unclear. Here, we found that the paucity of invariant natural killer T (iNKT) cells together with immaturity of the immune system was related to the...