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Experimental infection of a US spike-insertion deletion porcine epidemic diarrhea virus in conventional nursing piglets and cross-protection to the original US PEDV infection

Although the original US porcine epidemic diarrhea virus (PEDV) was confirmed as highly virulent by multiple studies, the virulence of spike-insertion deletion (S-INDEL) PEDV strains is undefined. In this study, 3–4 day-old conventional suckling piglets were inoculated with S-INDEL PEDV Iowa106 (4 pig litters) to study its virulence. Two litters of age-matched piglets were ...

Determination of the infectious titer and virulence of an original US porcine epidemic diarrhea virus PC22A strain

The infectious dose of a virus pool of original US PEDV strain PC22A was determined in 4-day-old, cesarean-derived, colostrum-deprived (CDCD) piglets. The median pig diarrhea dose (PDD 50 ) of the virus pool was determined as 7.35 log 10 PDD 50 /mL, similar to the cell culture infectious titer, 7.75 log 10 plaque-forming units (PFU)/mL. 100 PDD 50 caused watery diarrhea in all ...

The effect of infection order of porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus on dually infected swine alveolar macrophages

Background Concurrent infection with porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) is known as one of the major causes for porcine respiratory disease complex (PRDC). Dual infection with PCV2 and PRRSV is consistently to have more severe clinical presentations and pulmonary lesions than infection with PCV2 alone or PRRSV alone. ...

Postoperative coma in a patient with complete basilar syndrome after anterior cervical discectomy

Yung-Fong Tsai Anthony G. Doufas Cheng-Sen Huang Fu-Chao Liou Chun-Ming Lin md [Coma postopratoire chez un patient atteint d'un syndrome basilaire complet Purpose: Ischemic brainstem stroke

Recurrent seizure activity after epidural morphine in a post-partum woman

Chih-Jen Shih Anthony G. Doufas Hsu-Chu Chang Chun-Ming Lin [Activit pileptique rcurrente aprs une anesthsie pridurale avec morphine Purpose: We report on a primiparous woman who suffered recurrent