Bonn, Sigmund-Freud-Str. 25, 53127, Bonn, GermanyHendrik Lapp, Tobias Bruegmann, Daniela Malan, Stephanie Friedrichs, Carsten Kilgus & Philipp SasseResearch Training Group 1873, University of Bonn, 53127 ... • Google Scholar Search for Tobias Bruegmann in:Nature Research journals • PubMed • Google Scholar Search for Daniela Malan in:Nature Research journals • PubMed • Google Scholar Search for Stephanie
-Investing in your future) and the German federal state, North Rhine-Westphalia (NRW). Author Contributions Stephanie Friedrichs and Daniela Malan contributed equally to the work. Stephanie Friedrichs ... , Daniela Malan and Yvonne Voss performed the experiments and analyzed the data. Stephanie Friedrichs, Daniela Malan and Philipp Sasse designed the study and wrote the manuscript. Conflicts of Interest
Long QT syndrome is a potentially life-threatening disease characterized by delayed repolarization of cardiomyocytes, QT interval prolongation in the electrocardiogram, and a high risk for sudden cardiac death caused by ventricular arrhythmia. The genetic type 3 of this syndrome (LQT3) is caused by gain-of-function mutations in the SCN5A cardiac sodium channel gene which mediates...
In contrast to mature cardiomyocytes which have limited regenerative capacity, pluripotent stem cells represent a promising source for the generation of new cardiomyocytes. The tendency of pluripotent stem cells to form teratomas and the heterogeneity from various differentiation stages and cardiomyocyte cell sub-types, however, are major obstacles to overcome before this type of...
The electrical properties of the heart are primarily determined by the activity of ion channels and the activity of these molecules is permanently modulated and adjusted to the physiological needs by adrenergic signaling. miRNAs are known to control the expression of many proteins and to fulfill distinct functions in the mammalian heart, though the in vivo effects of miRNAs on...
Purification of cardiomyocytes from the embryonic mouse heart, embryonic stem (ES) or induced pluripotent stem cells (iPS) is a challenging task and will require specific isolation procedures. Lately the significance of surface markers for the isolation of cardiac cell populations with fluorescence activated cell sorting (FACS) has been acknowledged, and the hunt for cardiac...
Rationale Central questions such as cardiomyocyte subtype emergence during cardiogenesis or the availability of cardiomyocyte subtypes for cell replacement therapy require selective identification and purification of atrial and ventricular cardiomyocytes. However, current methodologies do not allow for a transgene-free selective isolation of atrial or ventricular cardiomyocytes...
Asifiqbal Kadari SubbaRao Mekala Nicole Wagner Daniela Malan Jessica Kth Katharina Doll Laura Stappert Daniela Eckert Michael Peitz Jan Matthes Philipp Sasse Stefan Herzig Oliver Brstle Sleyman Ergn ... Deutsche Forschungsgemeinschaft DFG (ED79/1-2), and the German Ministry of Education and Research, BMBF (01 GN 0813). Daniela Malan and Philipp Sasse were supported by the StemCellFactory project which is co
Aims Post-infarction remodelling (PIR) determines left-ventricular (LV) function and prognosis after myocardial infarction. The aim of this study was to evaluate transthoracic ultrasound-mediated microbubble stimulation (UMS) as a novel gene- and cell-free therapeutic option after acute myocardial infarction and reperfusion (AMI/R) in mice. Methods and Results For myocardial...
Dihydropyridines (DHP), which nifedipine is a member of, preferentially block Ca2+ channels of different cell types. Moreover, influx of Ca2+ through L-type Ca2+ channels (LTCCs) activates Ca2+ signaling pathways, which in turn contribute to numerous cellular processes. Although LTCCs are expressed in undifferentiated cells, very little is known about its contributions to the...
Diabetes mellitus (DM) encompasses a multitude of secondary disorders, including heart disease. One of the most frequent and potentially life threatening disorders of DM-induced heart disease is ventricular tachycardia (VT). Here we show that toll-like receptor 2 (TLR2) and NLRP3 inflammasome activation in cardiac macrophages mediate the production of IL-1β in DM mice. IL-1...