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Improving the temperature characteristics and catalytic efficiency of a mesophilic xylanase from Aspergillus oryzae, AoXyn11A, by iterative mutagenesis based on in silico design

To improve the temperature characteristics and catalytic efficiency of a glycoside hydrolase family (GHF) 11 xylanase from Aspergillus oryzae (AoXyn11A), its variants were predicted based on in silico design. Firstly, Gly21 with the maximum B-factor value, which was confirmed by molecular dynamics (MD) simulation on the three-dimensional structure of AoXyn11A, was subjected to...

Vertically Free-Standing Ordered Pb(Zr0.52Ti0.48)O3 Nanocup Arrays by Template-Assisted Ion Beam Etching

Zhang et al. Nanoscale Research Letters Vertically Free-Standing Ordered Pb(Zr Ti )O Nanocup Arrays by 0.52 0.48 3 Template-Assisted Ion Beam Etching Xiaoyan Zhang 0 Dan Tang 0 Kangrong Huang 0 Die

Contribution of Disulfide Bridges to the Thermostability of a Type A Feruloyl Esterase from Aspergillus usamii

The contribution of disulfide bridges to the thermostability of a type A feruloyl esterase (AuFaeA) from Aspergillus usamii E001 was studied by introducing an extra disulfide bridge or eliminating a native one from the enzyme. MODIP and DbD, two computational tools that can predict the possible disulfide bridges in proteins for thermostability improvement, and molecular dynamics...

Discriminating Males and Unpredictable Females: Males Differentiate Self-Similar Facial Cues More than Females in the Judgment of Opposite-Sex Attractiveness

Sen Zhang 0 Jing Xu 0 Die Hu 0 Alessio Avenanti, East China Normal University, China 0 Key Laboratory of Brain Functional Genomics (MOE & STCSM), School of Psychology and Cognitive Science, East China

Rituximab for Children with Immune Thrombocytopenia: A Systematic Review

5 patients but failed to contribute to safety analysis were excluded. Study Selection and Data Abstraction Two authors (Yi Liang and Die Hu) independently screened titles and abstracts of all ... review. We resolved disagreements by discussion until consensus is achieved. Two authors (Yi Liang and Die Hu) used a pre-designed data collection form to independently extract data from each included

A genome-wide association study identifies common variants influencing serum uric acid concentrations in a Chinese population

Background Uric acid (UA) is a complex phenotype influenced by both genetic and environmental factors as well as their interactions. Current genome-wide association studies (GWASs) have identified a variety of genetic determinants of UA in Europeans; however, such studies in Asians, especially in Chinese populations remain limited. Methods A two-stage GWAS was performed to...

Genome-wide association study on serum alkaline phosphatase levels in a Chinese population

Background Serum alkaline phosphatase (ALP) is a complex phenotype influenced by both genetic and environmental factors. Recent Genome-Wide Association Studies (GWAS) have identified several loci affecting ALP levels; however, such studies in Chinese populations are limited. We performed a GWAS analyzing the association between 658,288 autosomal SNPs and serum ALP in 1,461...

Imputation-based association analyses identify new lung cancer susceptibility variants in CDK6 and SH3RF1 and their interactions with smoking in Chinese populations

Cell cycle regulation, apoptosis, oxidative stress and inflammation response play critical roles in the development of smoking-induced lung cancer. However, it is still not well known whether their genetic variants are associated with lung cancer susceptibility. In this study, we performed imputation-based association analyses to investigate the influence of common genetic...