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Search: authors:"Du Toit W. P. Schabort"

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Gene regulation in Kluyveromyces marxianus in the context of chromosomes

statistical analyses. Author Contributions Conceptualization: Du Toit W. P. Schabort, James C. du Preez. Data curation: Du Toit W. P. Schabort. Formal analysis: Du Toit W. P. Schabort. Funding acquisition ... : Du Toit W. P. Schabort, Stephanus G. Kilian. Investigation: Du Toit W. P. Schabort. Methodology: Du Toit W. P. Schabort. Project administration: Stephanus G. Kilian. Resources: Stephanus G. Kilian

Elucidation of new condition-dependent roles for fructose-1,6-bisphosphatase linked to cofactor balances

The cofactor balances in metabolism is of paramount importance in the design of a metabolic engineering strategy and understanding the regulation of metabolism in general. ATP, NAD+ and NADP+ balances are central players linking the various fluxes in central metabolism as well as biomass formation. NADP+ is especially important in the metabolic engineering of yeasts for xylose...

Differential RNA-seq, Multi-Network Analysis and Metabolic Regulation Analysis of Kluyveromyces marxianus Reveals a Compartmentalised Response to Xylose

We investigated the transcriptomic response of a new strain of the yeast Kluyveromyces marxianus, in glucose and xylose media using RNA-seq. The data were explored in a number of innovative ways using a variety of networks types, pathway maps, enrichment statistics, reporter metabolites and a flux simulation model, revealing different aspects of the genome-scale response in an...