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Search: authors:"E. Gerhart H. Wagner"

8 papers found.
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RNA-based regulation in type I toxin–antitoxin systems and its implication for bacterial persistence

University , 75124 Uppsala , Sweden 1 Institut für Mikrobiologie und Molekularbiologie, Justus-Liebig-Universität , 35392 Giessen , Germany 2 E. Gerhart H. Wagner Bacterial dormancy is a valuable survival

Massive functional mapping of a 5′-UTR by saturation mutagenesis, phenotypic sorting and deep sequencing

Erik Holmqvist 0 Johan Reimega rd 0 E. Gerhart H. Wagner 0 0 Department of Cell and Molecular Biology, Biomedical Center, Uppsala University , SciLifeLab Uppsala, Box 596, S-75124 Uppsala, Sweden

Sigma E controls biogenesis of the antisense RNA MicA

Klas I. Udekwu 0 E. Gerhart H. Wagner 0 0 Department of Cell & Molecular Biology, Uppsala university, Biomedical Center , Box 596, S-75124 Uppsala, Sweden Adaptation stress responses in the Gram

The small RNA repertoire of Dictyostelium discoideum and its regulation by components of the RNAi pathway

Small RNAs play crucial roles in regulation of gene expression in many eukaryotes. Here, we report the cloning and characterization of 18–26 nt RNAs in the social amoeba Dictyostelium discoideum. This survey uncovered developmentally regulated microRNA candidates whose biogenesis, at least in one case, is dependent on a Dicer homolog, DrnB. Furthermore, we identified a large...

Sensory Ataxic Neuropathy in Golden Retriever Dogs Is Caused by a Deletion in the Mitochondrial tRNATyr Gene

Sensory ataxic neuropathy (SAN) is a recently identified neurological disorder in golden retrievers. Pedigree analysis revealed that all affected dogs belong to one maternal lineage, and a statistical analysis showed that the disorder has a mitochondrial origin. A one base pair deletion in the mitochondrial tRNATyr gene was identified at position 5304 in affected dogs after re...

RNomics in Escherichia coli detects new sRNA species and indicates parallel transcriptional output in bacteria

Recent bioinformatics‐aided searches have identified many new small RNAs (sRNAs) in the intergenic regions of the bacterium Escherichia coli. Here, a shot‐gun cloning approach (RNomics) was used to generate cDNA libraries of small sized RNAs. Besides many of the known sRNAs, we found new species that were not predicted previously. The present work brings the number of sRNAs in E...

Bulged residues promote the progression of a loop–loop interaction to a stable and inhibitory antisense–target RNA complex

In several groups of bacterial plasmids, antisense RNAs regulate copy number through inhibition of replication initiator protein synthesis. These RNAs are characterized by a long hairpin structure interrupted by several unpaired residues or bulged loops. In plasmid R1, the inhibitory complex between the antisense RNA (CopA) and its target mRNA (CopT) is characterized by a four...

A two unit antisense RNA cassette test system for silencing of target genes

This communication describes a two unit antisense RNA cassette system for use in gene silencing. Cassettes consist of a recognition unit and an inhibitory unit which are transcribed into a single RNA that carries sequences of non-contiguous complementarity to the chosen target RNA. The recognition unit is designed as a stem-loop for rapid formation of long-lived binding...