Advanced search    

Search: authors:"Edith Coonen"

18 papers found.
Use AND, OR, NOT, +word, -word, "long phrase", (parentheses) to fine-tune your search.

Europäische Datensammlung zur Präimplantationsdiagnostik seit 1999

Seit 25 Jahren gibt es die Präimplantationsdiagnostik (PID) als Alternative zur Pränataldiagnostik monogener, mitochondrialer und chromosomaler Erkrankungen. Nach In-vitro-Fertilisation (IVF) oder (meist) Intrazytoplasmatischer Spermieninjektion (ICSI) werden entweder Polkörperchen, Blastomere oder Ektodermzellen aus den Oozyten bzw. dem Präimplantationsembryo gewonnen, um sie...

Präimplantationsdiagnostik in den Niederlanden

Im Jahr 1995 wurde die Präimplantationsdiagnostik (PID) auf experimenteller Ebene in Maastricht eingeführt. Seit 2003 ist sie Bestandteil des Erstattungssystems des niederländischen Ministry Public Health, Welfare and Sport. PID wird nur Paaren ermöglicht, die mit einem Risiko für eine schwerwiegende monogene Erkrankung, strukturellen Chromosomenanomalien oder mitochondrialen...

Differences in gene expression profiles between human preimplantation embryos cultured in two different IVF culture media†

STUDY QUESTION Is gene expression in human preimplantation embryos affected by the medium used for embryo culture in vitro during an IVF treatment?

IVF culture medium affects post-natal weight in humans during the first 2 years of life†

STUDY QUESTION Is post-natal growth during the first 2 years of life in IVF singletons affected by type of medium used for culturing human embryos during an IVF treatment?

Further evidence that culture media affect perinatal outcome: findings after transfer of fresh and cryopreserved embryos

BACKGROUND We have previously shown that the medium used for culturing IVF embryos affects the birthweight of the resulting newborns. This observation with potentially far-reaching clinical consequences during later life, was made in singletons conceived during the first IVF treatment cycle after the transfer of fresh embryos. In the present study, we hypothesize that in vitro...

IVF culture medium affects human intrauterine growth as early as the second trimester of pregnancy†

STUDY QUESTION When does a difference in human intrauterine growth of singletons conceived after IVF and embryo culture in two different culture media appear?

What next for preimplantation genetic screening (PGS)? A position statement from the ESHRE PGD Consortium steering committee†

Since 2004, there have been 11 randomized controlled trials (RCTs) mainly for advanced maternal age (AMA), which have shown no benefit of performing preimplantation genetic screening (PGS). Ten of the RCTs have been performed at the cleavage stage and one at the blastocyst stage. It is probable that the high levels of chromosomal mosaicism at cleavage stages, which may result in...

Effect of in vitro culture of human embryos on birthweight of newborns

BACKGROUND In animal models, in vitro culture of preimplantation embryos has been shown to be a risk factor for abnormal fetal outcome, including high and low birthweight. In the human, mean birthweight of singletons after in vitro fertilization (IVF) is considerably lower than after natural conception, but it is not known whether culture conditions play a role in this. METHODS...

Elective single embryo transfer (eSET) policy in the first three IVF/ICSI treatment cycles

BACKGROUND: Elective single embryo transfer (eSET), applied in the first or second IVF cycle in young patients with good quality embryos, has been demonstrated to lower the twin pregnancy rate, while the overall pregnancy rate is not compromised. It is as yet unclear whether eSET could be the preferred transfer policy in all treatment cycles, or that it should be restricted to...

Growth rate of human preimplantation embryos is sex dependent after ICSI but not after IVF

BACKGROUND: There is concern that IVF and/or ICSI might have an adverse effect on embryonic development via epigenetic alterations. Such alterations might also be involved in the sex-related growth differences in preimplantation embryos found in some animal species. In the present study we analysed cell numbers of human male and female surplus embryos that developed to the...

Effect of oxygen concentration on human in-vitro fertilization and embryo culture*

In this prospective randomized study on 1380 consecutive in-vitro fertilization (IVF) treatments, the results were compared of culture of human oocytes and embryos for the first 2 or 3 days of development in microdroplets of medium under oil using a gas phase containing either atmospheric (~20%) or reduced (5%) O2 concentrations. No significant differences were found between the...

Anaphase lagging mainly explains chromosomal mosaicism in human preimplantation embryos

BACKGROUND: Cleavage stage embryos as well as postimplantation embryos have been studied extensively over the years. However, our knowledge with respect to the chromosomal constitution of human embryos at the blastocyst stage is still rudimentary. METHODS: In the present paper, a large series of human blastocysts was examined by means of fluorescent in situ hybridization (FISH...

Chromosomally abnormal cells are not selected for the extra‐embryonic compartment of the human preimplantation embryo at the blastocyst stage

BACKGROUND: Although well defined for embryos at cleavage stages, the occurrence and frequency of chromosomal aberrations in human blastocysts is relatively unknown. It has been reported that only one in four blastocysts is comprised totally of chromosomally normal cells. One of the selection mechanisms for the embryo proper to become free of these chromosomally abnormal cells...

Embryo development and chromosomal anomalies after ICSI: effect of the injection procedure*

Intracytoplasmic sperm injection (ICSI) is a delicate procedure requiring considerable skills of the person performing it. Theoretically, the injection procedure could damage cytoplasmic structures in the oocyte, resulting in sublethal cellular injury and/or numerical chromosomal abnormalities that could lead to impaired embryonic development. In the present study, features of...

Preimplantation genetic diagnosis of a reciprocal translocation t(3;11)(q27.3;q24.3) in siblings*

Preimplantation genetic diagnosis (PGD) was performed in two couples to avoid chromosomally unbalanced progeny in a family in which a brother and a sister carry an identical maternally inherited balanced translocation t(3;11)(q27.3;q24.3). Embryos were biopsied 3 days after fertilization and blastomeres were analysed by fluorescent in-situ hybridization (FISH). Embryos were...

Comparison of in-vitro development of embryos originating from either conventional in-vitro fertilization or intracytoplasmic sperm injection

In this retrospective study on 1628 consecutive cycles performed during a period of 4 years, development in vitro is compared of embryos obtained after either conventional in-vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). At 39–42 h after insemination or injection, embryos obtained after ICSI were significantly (P < 0.01) further developed (mean cell number...

Genetics: Preimplantation diagnosis for X and Y normality in embryos from three Klinefelter patients*

In some 47,XXY Klinefelter patients without evidence of mosaicism, testicular spermatozoa can be successfully recovered and used for intracytoplasmlc sperm Injection (ICSI). To ensure the replacement of embryos with a normal X and Y chromosome pattern, preimplantation diagnosis can be performed. This paper reports on three 47,XXY Klinefelter patients from whom it was possible to...