Developmental and Anatomical Patterns of IL-2 Gene Expression In Vivo in the Murine Thymus JULIA A. YANG-SNYDER 0 ELLEN V. ROTHENBERG 0 0 Division of Biology , 156-29 , California Institute of
Developmental and Anatomical Patterns of IL-2 Gene Expression In Vivo in the Murine Thymus JULIA A. YANG-SNYDER 0 ELLEN V. ROTHENBERG 0 0 Division of Biology , 156-29 , California Institute of
In situ hybridization and immunohistochemistry were used to determine the spectrum of tissues in which interleukin-2 (IL-2) mRNA and protein are found in healthy, normal young mice. In neonatal animals, IL-2 is expressed specifically by distinct, isolated cells at three major sites: the thymus, skin, and gut. Based on morphology and distribution, the IL-2-expressing cells...
In situ hybridization and immunohistochemistry were used to determine the spectrum of tissues in which interleukin-2 (IL-2) mRNA and protein are found in healthy, normal young mice. In neonatal animals, IL-2 is expressed specifically by distinct, isolated cells at three major sites: the thymus, skin, and gut. Based on morphology and distribution, the IL-2-expressing cells...
Developmental and Anatomical Patterns of IL-2 Gene Expression In Vivo in the Murine Thymus JULIA A. YANG-SNYDER 0 ELLEN V. ROTHENBERG 0 0 Division of Biology , 156-29 , California Institute of
In situ hybridization and immunohistochemistry were used to determine the spectrum of tissues in which interleukin-2 (IL-2) mRNA and protein are found in healthy, normal young mice. In neonatal animals, IL-2 is expressed specifically by distinct, isolated cells at three major sites: the thymus, skin, and gut. Based on morphology and distribution, the IL-2-expressing cells...
Developmental and Anatomical Patterns of IL-2 Gene Expression In Vivo in the Murine Thymus JULIA A. YANG-SNYDER 0 ELLEN V. ROTHENBERG 0 0 Division of Biology , 156-29 , California Institute of
Three interlocking problems in gene regulation are: how to explain genome-wide targeting of transcription factors in different cell types, how prior transcription factor action can establish an ‘epigenetic state’ that changes the options for future transcription factor action, and how directly a sequence of developmental decisions can be memorialized in a hierarchy of repression...
Background/Objectives A cascade of gene activations under the control of Notch signalling is required during T-cell specification, when T-cell precursors gradually lose the potential to undertake other fates and become fully committed to the T-cell lineage. We elucidate how the gene/protein dynamics for a core transcriptional module governs this important process by computational...
Marissa Morales Del Real 0 Ellen V. Rothenberg 0 0 Division of Biology 156-29, California Institute of Technology , Pasadena, CA 91125 , USA SUMMARY Hematopoiesis is a classic system with which to
Erica Manesso Vijay Chickarmane Hao Yuan Kueh Ellen V. Rothenberg Carsten Peterson 0 Division of Biology, California Institute of Technology , Pasadena, CA, USA 1 Computational Biology and Biological
Satoko Adachi 0 Ellen V. Rothenberg 0 0 Division of Biology 156-29, California Institute of Technology , Pasadena, CA 91125, USA T-cells retain cell-type-specific programming for IL-2 inducibility
The gradual reprogramming of haematopoietic precursors into the T-cell fate is characterized by at least two sequential developmental stages. Following Notch1-dependent T-cell lineage specification during which the first T-cell lineage genes are expressed and myeloid and dendritic cell potential is lost, T-cell specific transcription factors subsequently induce T-cell commitment...
A central question in T cell development is what makes cortical thymocytes respond to stimulation in a qualitatively different way than any other thymocyte subset. Part of the answer is that AP-1 function changes drastically at two stages of T cell development. It undergoes striking down-regulation as thymocytes differentiate from immature, CD4–CD8– double-negative (DN) TCR...
Known transcription factor-DNA interactions in the minimal enhancer of the murine interleukin-2 gene (IL-2) do not easily explain the T cell specificity of IL-2 regulation. To seek additional determinants of cell type specificity, in vivo methodologies were employed to examine chromatin structure 5′ and 3′ of the 300 bp IL-2 proximal promoter/enhancer region. Restriction enzyme...
Cartilaginous fish express canonical B and T cell recognition genes, but their lymphoid organs and lymphocyte development have been poorly defined. Here, the expression of Ig, TCR, recombination-activating gene (Rag)-1 and terminal deoxynucleosidase (TdT) genes has been used to identify roles of various lymphoid tissues throughout development in the cartilaginous fish, Raja...
