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Transcription of the mating-type-regulated lncRNA IRT1 is governed by TORC1 and PKA

Institute , 44 Lincoln's Inn Fields, London WC2A 3LY , UK 1 Communicated by M. Kupiec 2 Folkert J. van Werven Cell fate decisions are controlled by multiple cell-intrinsic and -extrinsic factors. In budding

Regulation of entry into gametogenesis

Folkert J. van Werven 0 Angelika Amon () 0 0 David H. Koch Institute for Integrative Cancer Research and Howard Hughes Medical Institute, Massachusetts Institute of Technology , Cambridge, MA 02139

The use of biotin tagging in Saccharomyces cerevisiae improves the sensitivity of chromatin immunoprecipitation

Folkert J. van Werven 0 H. Th. Marc Timmers 0 0 Department of Physiological Chemistry, Division of Biomedical Genetics, University Medical Centre Utrecht, Universiteitsweg 100

Improved genome-wide localization by ChIP-chip using double-round T7 RNA polymerase-based amplification

Chromatin immunoprecipitation combined with DNA microarrays (ChIP-chip) is a powerful technique to detect in vivo protein–DNA interactions. Due to low yields, ChIP assays of transcription factors generally require amplification of immunoprecipitated genomic DNA. Here, we present an adapted linear amplification method that involves two rounds of T7 RNA polymerase amplification ...