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A viral metagenomic approach on a non-metagenomic experiment: Mining next generation sequencing datasets from pig DNA identified several porcine parvoviruses for a retrospective evaluation of viral infections

Shot-gun next generation sequencing (NGS) on whole DNA extracted from specimens collected from mammals often produces reads that are not mapped (i.e. unmapped reads) on the host reference genome and that are usually discarded as by-products of the experiments. In this study, we mined Ion Torrent reads obtained by sequencing DNA isolated from archived blood samples collected from ...

Periweaning Failure to Thrive Syndrome (PFTS): Is There a Genetic Component?

663, ASL R3183. Available at: 2 Iowa State University , USA 3 Francesca Bertolini, Postdoctoral Research Associate, ISU; Tianfu Yang, PhD Candidate, University of Alberta, Canada Yanyun Huang, Prairie

Exploring gastric bacterial community in young pigs

Microbiota plays an important role in the homeostasis of the gastrointestinal tract. Understanding the variations of the commensal microbiota composition is crucial for a more efficient control of enteric infectious diseases and for the reduction of the use of antibiotics in animal production, which are the main points of interest for improved animal healthcare and welfare and for ...

Evidence of Natural Selection Footprints Among Some African Chicken Breeds and Village Ecotypes

Village Ecotypes Authors Ahmed R. Elbetagy, Francesca Bertolini, Damarius S. Fleming, Angelica Van Goor, Carl .. Schmidt, Susan J. Lamont, and Max F. Rothschild Thi s dairy is available in Animal Industry ... Scholar, ISU; Senior Researcher, APRI, Egypt; Francesca Bertolini, Postdoctoral Research Associate, ISU; Damarius S. Fleming, PhD Candidate, ISU and Postdoctoral Research Associate, USDA-ORAU; Angelica

Genomic Differences between Preweaning Survival and Mortality of Piglets Following PEDV Outbreaks

Porcine Epidemic Diarrhea (PED) is a serious and potentially devastating disease in the US and Canadian pig industries. To investigate if there is genetic resistance to PEDV a total of 156 dead and 106 surviving neonatal piglets infected with PEDV were genotyped with over 60,000 genetic markers to investigate genetic differences between the two groups. This allows for the ...

Next Generation Semiconductor Based Sequencing of the Donkey (Equus asinus) Genome Provided Comparative Sequence Data against the Horse Genome and a Few Millions of Single Nucleotide Polymorphisms

Few studies investigated the donkey (Equus asinus) at the whole genome level so far. Here, we sequenced the genome of two male donkeys using a next generation semiconductor based sequencing platform (the Ion Proton sequencer) and compared obtained sequence information with the available donkey draft genome (and its Illumina reads from which it was originated) and with the EquCab2.0 ...

A Next Generation Semiconductor Based Sequencing Approach for the Identification of Meat Species in DNA Mixtures

The identification of the species of origin of meat and meat products is an important issue to prevent and detect frauds that might have economic, ethical and health implications. In this paper we evaluated the potential of the next generation semiconductor based sequencing technology (Ion Torrent Personal Genome Machine) for the identification of DNA from meat species (pig, horse, ...