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11 papers found.
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SMC complexes orchestrate the mitotic chromatin interaction landscape

Communicated by M. Kupiec 2 Frank Uhlmann Chromatin is a very long DNA-protein complex that controls the expression and inheritance of the genetic information. Chromatin is stored within the nucleus in

Nur1 Dephosphorylation Confers Positive Feedback to Mitotic Exit Phosphatase Activation in Budding Yeast

Substrate dephosphorylation by the cyclin-dependent kinase (Cdk)-opposing phosphatase, Cdc14, is vital for many events during budding yeast mitotic exit. Cdc14 is sequestered in the nucleolus through inhibitory binding to Net1, from which it is released in anaphase following Net1 phosphorylation. Initial Net1 phosphorylation depends on Cdk itself, in conjunction with proteins of ...

A simple biophysical model emulates budding yeast chromosome condensation

Bates 2 Frank Uhlmann 1 0 Advanced Sequencing Facility, Lincoln's Inn Fields Laboratory, The Francis Crick Institute , London , United Kingdom 1 Chromosome Segregation Laboratory, Lincoln's Inn Fields

An Eco1-independent sister chromatid cohesion establishment pathway in S. cerevisiae

Cohesion between sister chromatids, mediated by the chromosomal cohesin complex, is a prerequisite for their alignment on the spindle apparatus and segregation in mitosis. Budding yeast cohesin first associates with chromosomes in G1. Then, during DNA replication in S-phase, the replication fork-associated acetyltransferase Eco1 acetylates the cohesin subunit Smc3 to make cohesin’s ...

A quantitative model for cyclin-dependent kinase control of the cell cycle: revisited

Frank Uhlmann () 0 Cline Bouchoux 0 Sandra Lpez-Avils 0 0 Chromosome Segregation Laboratory, Cancer Research UK London Research Institute , 44 Lincoln's Inn Fields, London WC2A 3LY , UK Articles on

Cohesin loading and sliding

Maria T. Ocampo-Hafalla 0 Frank Uhlmann () 0 0 Chromosome Segregation Laboratory, Cancer Research UK London Research Institute , Lincoln's Inn Fields Laboratories, 44 Lincoln's Inn Fields, London

Conserved features of cohesin binding along fission yeast chromosomes

Background Cohesin holds sister chromatids together to enable their accurate segregation in mitosis. How, and where, cohesin binds to chromosomes are still poorly understood, and recent genome-wide surveys have revealed an apparent disparity between its chromosomal association patterns in different organisms. Results Here, we present the high-resolution analysis of cohesin ...

Computational modelling of mitotic exit in budding yeast: the role of separase and Cdc14 endocycles

P. K. Vinod Paula Freire Ahmed Rattani Andrea Ciliberto Frank Uhlmann Bela Novak 0 IFOMThe FIRC Institute of Molecular Oncology , via Adamello 16, 20139 Milan, Italy 1 PDBC, Instituto Gulbenkian de

Displacement and re-accumulation of centromeric cohesin during transient pre-anaphase centromere splitting

The ring-shaped cohesin complex links sister chromatids until their timely segregation during mitosis. Cohesin is enriched at centromeres where it provides the cohesive counterforce to bipolar tension produced by the mitotic spindle. As a consequence of spindle tension, centromeric sequences transiently split in pre-anaphase cells, in some organisms up to several micrometers. This ...

DNA recognition properties of the N-terminal DNA binding domain within the large subunit of Replication Factor C

Replication Factor C (RFC) is a five-subunit protein complex required for eukaryotic DNA replication and repair. The large subunit within this complex contains a C-terminal DNA binding domain which provides specificity for PCNA loading at a primer-template and a second, N-terminal DNA binding domain of unknown function. We isolated the N-terminal DNA binding domain from Drosophila ...