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Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans

Polysaccharides, the most abundant biopolymers, are required for a host of activities in lower organisms, animals, and plants. Their solution characterization is challenging due to their complex shape, heterogeneity, and size. Here, recently developed data analysis approaches were applied for traditional sedimentation equilibrium and velocity methods in order to investigate the...

Assessing sedimentation equilibrium profiles in analytical ultracentrifugation experiments on macromolecules: from simple average molecular weight analysis to molecular weight distribution and interaction analysis

conflicts of interest. Gary G. Adams declares that he has no conflicts of interest. Ethical approval This article does not contain any studies with human participants or animals performed by any of the

Characterisation of insulin analogues therapeutically available to patients

hydrodynamic aspects of the findings. Author Contributions Conceptualization: Gary G. Adams, Andrew Meal, Paul S. Morgan, M. Samil Kok, Stephen E. Harding. Data curation: Qushmua E. Alzahrani, Hanne Zobel ... , Ryan Lithgo, David T. M. Besong, Shahwar I. Jiwani, Simon Ballance, Stephen E. Harding, Richard B. Gillis. Formal analysis: Gary G. Adams, Andrew Meal, Paul S. Morgan, Hanne Zobel, Ryan Lithgo, M. Samil

Controlled depolymerisation assessed by analytical ultracentrifugation of low molecular weight chitosan for use in archaeological conservation

The heterogeneity and molecular weight of a chitosan of low molecular weight (molar mass) and low degree of acetylation (0.1) for potential use as a consolidant for decayed archaeological wood were examined by sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge before and after depolymerisation. Sedimentation velocity before depolymerisation...

Full hydrodynamic reversibility of the weak dimerization of vancomycin and elucidation of its interaction with VanS monomers at clinical concentration

5RD, United KingdomRyan Lithgo, Vlad Dinu, Richard B. Gillis, Gary G. Adams & Stephen E. HardingDepartment of Architecture and the Built Environment, The University of the West of England, Bristol, BS16 ... 1QY, United KingdomJohn E. HardingSchool of Health Sciences, University of Nottingham, Nottingham, NG7 2HA, United KingdomRichard B. Gillis & Gary G. Adams AuthorsSearch for Mary K. Phillips-Jones

MultiSig: a new high-precision approach to the analysis of complex biomolecular systems

MultiSig is a newly developed mode of analysis of sedimentation equilibrium (SE) experiments in the analytical ultracentrifuge, having the capability of taking advantage of the remarkable precision (~0.1 % of signal) of the principal optical (fringe) system employed, thus supplanting existing methods of analysis through reducing the ‘noise’ level of certain important parameter...

Protocol-directed insulin infusion sliding scales improve perioperative hyperglycaemia in critical care

Perioperative hyperglycaemia is associated with poor outcomes in patients undergoing cardiac surgery. Frequent postoperative hyperglycaemia in cardiac surgery patients has led to the initiation of an insulin infusion sliding scale for quality improvement. A systematic review was conducted to determine whether a protocol-directed insulin infusion sliding scale is as safe and...

Use of the Extended Fujita method for representing the molecular weight and molecular weight distributions of native and processed oat beta-glucans

University of Nottingham, Sutton Bonington, LE12 5RD, UKGuy A. Channell, Gary G. Adams, YuDong Lu, Richard B. Gillis, Vlad Dinu & Stephen E. HardingFaculty of Medicine and Health Sciences, Queens Medical ... , St. Olavs plass, 0130, Oslo, NorwayStephen E. Harding AuthorsSearch for Guy A. Channell in:Nature Research journals • PubMed • Google Scholar Search for Gary G. Adams in:Nature Research journals