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Introgressive Hybridization in Potato Revealed by Novel Cytogenetic and Genomic Technologies

Agronomia, Universidad de la Republica , Garzon 780, 12900 Montevideo , Uruguay 2 Hans de Jong Potato is the third most important food crop in the world and is crucial to ensure food security. However

Two reported cytotypes of the emergent orchid model species Erycina pusilla are two different species

Each species is characterized by a specific set of chromosomes, which is described as the chromosome portrait or karyotype. In general, such a karyotype is the same for all individuals in the population. An exception to that rule has recently been found in the orchid Erycina pusilla, which has been reported to have two cytotypes with chromosome numbers of 2n = 10 and 2n = 12...

Pairing analysis and in situ Hybridisation reveal autopolyploid-like behaviour in Solanum commersonii × S. tuberosum (potato) interspecific hybrids

Wild potato relatives are rich sources of desirable traits for introgressive hybridisation into cultivated potato. One of them, Solanum commersonii (2n = 2x = 24, 1EBN, endosperm balance number), is an important species belonging to the potato tertiary genepool. It can be used in potato breeding through bridge crosses and 2n gamete production. Triploid F1 hybrids between S...

Meiotic analysis and FISH with rDNA and rice BAC probes of the Thai KPS 01-01-25 sugarcane cultivar

Campus, Kamphaeng Saen, Nakhon Pathom 73140 , Thailand 1 Institute for Advanced Studies, Center for Advanced Studies in Tropical Natural Resources, Kasetsart University , Bangkok , Thailand 2 & Hans de ... Jong 3 Laboratory of Genetics, Plant Science Group, Wageningen University , Wageningen , The Netherlands 4 Laboratory of Biology, Kasetsart University , Kamphaeng Sean Campus, Kamphaeng Saen , Thailand 5

Cnidaria: fast, reference-free clustering of raw and assembled genome and transcriptome NGS data

University Sander Peters: PhD in Bioinformatics - Senior scientist/Bioinformatician at Plant Research International – Wageningen University Hans de Jong: Professor of cytogenetics at Wageningen University Dick

Homologues of potato chromosome 5 show variable collinearity in the euchromatin, but dramatic absence of sequence similarity in the pericentromeric heterochromatin

Background In flowering plants it has been shown that de novo genome assemblies of different species and genera show a significant drop in the proportion of alignable sequence. Within a plant species, however, it is assumed that different haplotypes of the same chromosome align well. In this paper we have compared three de novo assemblies of potato chromosome 5 and report on the...

Fine mapping of the tomato yellow leaf curl virus resistance gene Ty-2 on chromosome 11 of tomato

Resistances to begomoviruses, including bipartite tomato mottle virus and monopartite tomato yellow leaf curl virus (TYLCV), have been introgressed to cultivated tomato (Solanum lycopersicum) from wild tomato accessions. A major gene, Ty-2 from S. habrochaites f. glabratum accession “B6013,” that confers resistance to TYLCV was previously mapped to a 19-cM region on the long arm...

The origin of a selfish B chromosome triggering paternal sex ratio in the parasitoid wasp Trichogramma kaykai

This study uses molecular and cytogenetic methods to determine the origin of a B chromosome in some males of the wasp Trichogramma kaykai. This so-called paternal sex ratio (PSR) chromosome transmits only through sperm and shortly after fertilization triggers degeneration of the paternal genome, while keeping itself intact. The resulting embryos develop into haploid B-chromosome...

The genomic landscape of meiotic crossovers and gene conversions in Arabidopsis thaliana

Knowledge of the exact distribution of meiotic crossovers (COs) and gene conversions (GCs) is essential for understanding many aspects of population genetics and evolution, from haplotype structure and long-distance genetic linkage to the generation of new allelic variants of genes. To this end, we resequenced the four products of 13 meiotic tetrads along with 10 doubled haploids...

Assignment of genetic linkage maps to diploid Solanum tuberosum pachytene chromosomes by BAC-FISH technology

Xiaomin Tang Jan M. de Boer Herman J. van Eck Christian Bachem Richard G. F. Visser Hans de Jong BAC CCD Cot Responsible Editor: Jiming Jiang. 0 ) Laboratory of Genetics, Wageningen University , P.O

The potential of high-resolution BAC-FISH in banana breeding

The genetic complexity in the genus Musa has been subject of study in many breeding programs worldwide. Parthenocarpy, female sterility, polyploidy in different cultivars and limited amount of genetic and genomic information make the production of new banana cultivars difficult and time consuming. In addition, it is known that part of the cultivars and related wild species in the...

Map- vs. homology-based cloning for the recessive gene ol-2 conferring resistance to tomato powdery mildew

The recessive gene ol-2 confers papilla-associated and race-non-specific resistance to tomato powdery mildew caused by Oidium neolycopersici. In order to facilitate marker assisted selection (MAS) in practical breeding programmes, we identified two simple sequence repeat (SSR) markers and one cleaved amplified polymorphic sequence (CAPS) marker which are linked to the resistance...