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16 papers found.
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Particle segmentation algorithm for flexible single particle reconstruction

As single particle cryo-electron microscopy has evolved to a new era of atomic resolution, sample heterogeneity still imposes a major limit to the resolution of many macromolecular complexes, especially those with continuous conformational flexibility. Here, we describe a particle segmentation algorithm towards solving structures of molecules composed of several parts that are...

4.4 Å Resolution Cryo-EM structure of human mTOR Complex 1

Jiawei Wang 5 Yanhui Xu 0 1 2 Hong-Wei Wang 5 0 State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, School of Life Sciences, Fudan University ... financial interests. COMPLIANCE WITH ETHICS GUIDELINES Huirong Yang, Jia Wang, Mengjie Liu, Xizi Chen, Min Huang, Dan Tan, Meng-Qiu Dong, Catherine C. L. Wong, Jiawei Wang, Yanhui Xu and Hong-Wei Wang

Structure of precursor microRNA’s terminal loop regulates human Dicer’s dicing activity by switching DExH/D domain

and Hong-Wei Wang declare that they have no conflict of interest. This article does not contain any studies with human or animal subjects performed by the any of the authors. This article is

Evaluation of Liver Ischemia-Reperfusion Injury in Rabbits Using a Nanoscale Ultrasound Contrast Agent Targeting ICAM-1

Objective To assess the feasibility of ultrasound molecular imaging in the early diagnosis of liver ischemia-reperfusion injury (IRI) using a nanoscale contrast agent targeting anti-intracellular adhesion molecule-1 (anti-ICAM-1). Methods The targeted nanobubbles containing anti-ICAM-1 antibody were prepared using the avidin-biotin binding method. Human hepatic sinusoidal...

Effects of Miglitol, Acarbose, and Sitagliptin on Plasma Insulin and Gut Peptides in Type 2 Diabetes Mellitus: A Crossover Study

. Hiroaki Ueno, Wakaba Tsuchimochi, Hong-Wei Wang, Eiichiro Yamashita, Chikako Tsubouchi, Kazuhiro Nagamine, Hideyuki Sakoda, and Masamitsu Nakazato declare no conflicts of interest. Compliance with ethics

Nuclear factor-κB activation in perihematomal brain tissue correlates with outcome in patients with intracerebral hemorrhage

Background Nuclear factor-κB (NF-κB) plays an important role in the inflammatory response after intracerebral hemorrhage (ICH). We therefore proposed that NF-κB activation in perihematomal brain tissue might correlate with clinical outcome in patients with ICH. To confirm this, we studied clinical data of 45 patients with ICH and NF-κB activation in perihematomal brain tissue and...

The M235T polymorphism in the angiotensinogen gene and heart failure: a meta-analysis

textbooks, review articles and included articles were identified through manual searches to find other potentially eligible studies. Data extraction Two investigators (Song Chen and Hong-Wei Wang) extracted

New triterpenoids from the kernels of Azadirachta indica

Three new limonoids (1–3) and a new intact triterpenoid (4), along with three known constituents (5–7), were isolated from the dried kernels (after extracting azadirachtin) of Azadirachta indica. The structures of the new compounds 1-benzoyl-3-deacetyl-1-detigloyl salannin (1), 7-tigloyl-12-oxo vilasini (2), azadiralactone (3) and azadirahemiacetal (4) were elucidated by means of...

Mechanistic insights into the role of Hop2–Mnd1 in meiotic homologous DNA pairing

The Hop2–Mnd1 complex functions with the DMC1 recombinase in meiotic recombination. Hop2–Mnd1 stabilizes the DMC1-single-stranded DNA (ssDNA) filament and promotes the capture of the double-stranded DNA partner by the recombinase filament to assemble the synaptic complex. Herein, we define the action mechanism of Hop2–Mnd1 in DMC1-mediated recombination. Small angle X-ray...

Structural insight of a concentration-dependent mechanism by which YdiV inhibits Escherichia coli flagellum biogenesis and motility

YdiV is a negative regulator of cell motility. It interacts with FlhD4C2 complex, a product of flagellar master operon, which works as the transcription activator of all other flagellar operons. Here, we report the crystal structures of YdiV and YdiV2–FlhD2 complex at 1.9 Å and 2.9 Å resolutions, respectively. Interestingly, YdiV formed multiple types of complexes with FlhD4C2...

MicroRNA-377 inhibited proliferation and invasion of human glioblastoma cells by directly targeting specificity protein 1

Background Increasing evidence has indicated that microRNAs (miRNAs) are strongly implicated in the initiation and progression of glioblastoma multiforme (GBM). Here, we identified a novel tumor suppressive miRNA, miR-377, and investigated its role and therapeutic effect for GBM.

Simulations of Tubulin Sheet Polymers as Possible Structural Intermediates in Microtubule Assembly

The microtubule assembly process has been extensively studied, but the underlying molecular mechanism remains poorly understood. The structure of an artificially generated sheet polymer that alternates two types of lateral contacts and that directly converts into microtubules, has been proposed to correspond to the intermediate sheet structure observed during microtubule assembly...

Analysis of specialized DNA polymerases expression in human gliomas: association with prognostic significance

Aberrant activation of the translesion DNA synthesis (TLS) pathway has been suggested to play a role in tumorigenesis by promoting genetic mutations. We therefore examined glioma specimens for the expression of specialized DNA polymerases involved in TLS and assessed their prognostic significance. The expression levels of DNA polymerase κ (Pol κ), Pol ι, and Pol η were assessed...

Lack of association between mannose-binding lectin gene polymorphisms and juvenile idiopathic arthritis in a Han population from the Hubei province of China

Corresponding author: Hong-Wei Wang 0 Chinese Center for Disease Control and Prevention , 27 Nan Wei Road, Beijing 100050 , China 1 Department of Pediatrics, Tongji Hospital, Tongji Medical College